LOCUS       sequence-444353-        2274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0244
FEATURES             Location/Qualifiers
     source          1..2274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     150..169
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     397..415
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(419..462)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      712..769
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(793..810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(964..1552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1044..1063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1735..1829)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1850..2274,1..235))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
       61 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      121 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      181 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      241 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      301 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
      361 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
      421 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagga
      481 gggaggcgtg tcgttttagt gaggaagagg ttccaaccac gtctacaaaa ccattattgc
      541 gcgttcaaaa gctaaaacat tgctttgtca aaagctaaaa aagatgatgc ccgacagcca
      601 gacgaaggaa atgacgtaag ccatgacgtc taatcccaca agctatataa ggttttgcta
      661 ttcattgaaa gcagtagtga ctgatttgta tatatactcg agaccctgca gtccggcaaa
      721 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
      781 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      961 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1381 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc
     1741 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1801 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1861 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1921 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1981 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     2041 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     2101 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     2161 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     2221 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgt
//
