LOCUS       sequence-44439-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS191
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..410
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     414..430
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     414..430
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(464..480)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(465..481)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 aagctggagc tcatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc
       61 ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc
      121 tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga
      181 gaccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt
      241 tttcttgaaa attttttttt tgattttttt ctctttcgat gacctcccat tgatatttaa
      301 gttaataaac ggtcttcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac
      361 tttttttact tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttctagaa
      421 ctagtggatc ccccgggctg caggaattgg atatcaagct tatcgatacc gtcgacctcg
      481 acaatgccag aacttggatt tagcatatca ggtggtgtcg ggggtagagg aaacccattc
      541 agacctgatg atgatggtat atttgtaaca agggtacaac ctgaaggacc agcatcaaaa
      601 ttactgcagc caggtgataa aattattcag gctaatggct acagttttat aaatattgaa
      661 catggacaag cagtgtcctt gctaaaaact ttccagaata cagttgaact catcattgta
      721 cgagaagttg gtaacggtgc aaaacaagag attcgagtga gggttgaaaa agacggtggt
      781 tctggaggcg tttcttctgt tccgaccaat ctgga
//
