LOCUS       sequence-44440-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a low-affinity ePDZ variant fused to mCherry in yeast. 
            Illumination with 440 nm light induces binding to LOVpep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS220
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     390..406
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     390..406
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tgtttctact ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac
       61 ttcaaaacac ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt
      121 aattacccgt actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg
      181 tcgaaaaagg caataaaaat ttttatcacg tttctttttc ttgaaaattt tttttttgat
      241 ttttttctct ttcgatgacc tcccattgat atttaagtta ataaacggtc ttcaatttct
      301 caagtttcag tttcattttt cttgttctat tacaactttt tttacttctt gctcattaga
      361 aagaaagcat agcaatctaa tctaagtttt ctagaactag tggatccccc gggctgcagg
      421 aattcgatat caagcttatc gataccgtcg acaatgccag aacttggatt tagcatatca
      481 ggtggtgtcg ggggtagagg aaacccattc agacctgatg atgatggtat atttgtaaca
      541 agggtacaac ctgaaggacc agcatcaaaa ttactgcagc caggtgataa aattattcag
      601 gctaatggct acagttttat aaatattgaa catggacaag cagtgtcctt gctaaaaact
      661 ttccagaata cagttgaact catcattgta cgagaagttg gtaacggtgc aaaacaagag
      721 attcgagtga gggttgaaaa agacggtggt tctggaggcg tttcttctgt tccgaccaat
      781 ctggaagttg ttgctgcgac cccgactagc ctgctgatca
//
