LOCUS       sequence-44441-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a high-affinity ePDZ variant fused to mCherry in yeast. 
            Illumination with 440 nm light induces binding to LOVpep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS221
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     389..405
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     389..405
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtttctactc cttttttact cttccagatt ttctcggact ccgcgcatcg ccgtaccact
       61 tcaaaacacc caagcacagc atactaaatt tcccctcttt cttcctctag ggtgtcgtta
      121 attacccgta ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt
      181 cgaaaaaggc aataaaaatt tttatcacgt ttctttttct tgaaaatttt ttttttgatt
      241 tttttctctt tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc
      301 aagtttcagt ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa
      361 agaaagcata gcaatctaat ctaagttttc tagaactagt ggatcccccg ggctgcagga
      421 attcgatatc aagcttatcg ataccgtcga caatgccaga acttggattt agcatatcag
      481 gtggtgtcgg gggtagagga aacccattca gacctgatga tgatggtata tttgtaacaa
      541 gggtacaacc tgaaggacca gcatcaaaat tactgcagcc aggtgataaa attattcagg
      601 ctaatggcta cagttttata aatattgaac atggacaagc agtgtccttg ctaaaaactt
      661 tccagaatac agttgaactc atcattgtac gagaagttgg taacggtgca aaacaagaga
      721 ttcgagtgag ggttgaaaaa gacggtggtt ctggaggcgt ttcttctgtt ccgaccaatc
      781 tggaagttgt tgctgcgacc ccgactagcc tgctgatcag ctgg
//
