LOCUS       sequence-44444-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, 
            Appella E, Kastan MB, Siliciano JD
  TITLE     Activation of the ATM kinase by ionizing radiation and 
            phosphorylation of p53.
  JOURNAL   Science. 1998 Sep 11;281(5383):1677-9.
  PUBMED    9733515
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1677-9"
COMMENT     Sequence Label: pcDNA3.1(+) Flag-His-ATM kd
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..84)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(88..111)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(213..232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(214..232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(277..480)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(306..326)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        481..785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttctgtagct ctatcatgtt ctagttgacg gcagcagata agcagatcat taagtactag
       61 actcatatga tgatgatgat gatgcatctt gtcgtcatcg tccttgtagt ccatggtggt
      121 cgagcggccg ccagtgtgat ggatatctgc agaattccag cacactggcg gccgttacta
      181 gtggatccga gctcggtacc aagcttgggt ctccctatag tgagtcgtat taatttcgat
      241 aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca
      301 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
      361 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
      421 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
      481 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
      541 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
      601 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
      661 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
      721 cgtcaatggg cgggggtcgt tggggcggtc agccaggcgg gccatttacc gtaagttatg
      781 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
      841 aactagtcaa taatcaaatg tcacgcg
//
