LOCUS       sequence-44446-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS282
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    118..170
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(515..531)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(515..531)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtggc gaccggtgga
      121 tcccgggccc gcggtaccgt cgattgcaga attcgaagct tgagctcgag tctagagtct
      181 ttttcaaccc tcactcgaat ctcttgtttt gcaccgttac caacttctcg tacaatgatg
      241 agttcaactg tattctggaa agtttttagc aaggacactg cttgtccatg ttcaatattt
      301 ataaaactgt agccattagc ctgaataatt ttatcacctg gctgcagtaa ttttgatgct
      361 ggtccttcag gttgtaccct tgttacaaat ataccatcat catcaggtct gaatgggttt
      421 cctctacccc cgacaccacc tgatatgcta aatccaagtt ctggcattgt cgacggtatc
      481 gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag aaaacttaga
      541 ttagattgct atgctttctt tctaatgagc aagaagtaaa aaaagttgta atagaacaag
      601 aaaaatgaaa ctgaaacttg agaaattgaa gaccgtttat taacttaaat atcaatggga
      661 ggtcatcgaa agagaaaaaa atcaaaaaaa aaattttcaa gaaaaagaaa cgtgataaaa
      721 atttttattg cctttttcga cgaagaaaaa gaaacgaggc ggtctctttt ttcttt
//
