LOCUS       sequence-44448-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS296
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(52..68)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(53..69)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(682..700)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 cggccgcact agtggatccc ccgggctgca ggaattggat atcaagctta tcgataccgt
       61 cgacctcgac aatgccagaa cttggattta gcatatcagg tggtgtcggg ggtagaggaa
      121 acccattcag acctgatgat gatggtatat ttgtaacaag ggtacaacct gaaggaccag
      181 catcaaaatt actgcagcca ggtgataaaa ttattcaggc taatggctac agttttataa
      241 atattgaaca tggacaagca gtgtccttgc taaaaacttt ccagaataca gttgaactca
      301 tcattgtacg agaagttggt aacggtgcaa aacaagagat tcgagtgagg gttgaaaaag
      361 acggtggttc tggaggcgtt tcttctgttc cgaccaatct ggaagttgtt gctgcgaccc
      421 cgactagcct gctgatcagc tgggatgcgt atagggagct tccggttagt tattaccgta
      481 tcacgtacgg tgaaaccggt ggtaactccc cggttcagga attcactgta cctggttcca
      541 agtctactgc taccatcagc ggcctgaaac cgggtgttga ctataccatc actgtatacg
      601 cacattacaa ctaccattac tactctagcc caatctcgat taactaccgt acctctagac
      661 tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct
      721 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
      781 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
      841 cgtgtacgca
//
