LOCUS       sequence-444496-        9221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human integrin alpha4 full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, 
            Springer TA
  TITLE     Ligand binding initiates single-molecule integrin conformational 
            activation.
  JOURNAL   Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 
            10.1016/j.cell.2024.04.049.
  PUBMED    38772370
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049."
COMMENT     Sequence Label: pcDNA3.4 human alpha4 full
FEATURES             Location/Qualifiers
     source          1..9221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      729..738
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3837..3860
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(3870..3875,3876..3899,3900..3935,3936..3959)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     misc_feature    3998..4586
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4051..4071)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4469..4480)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4607..4626)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4651..4699
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4901..5329
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4988..5007)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5198..5219
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5338..5358)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5343..5672
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5523..5658
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5585..5604
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5739..6533
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5793..5812)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6403..6422
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6709..6830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6746..6765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6800..6819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6879..6895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6879..6895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6892..6914)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6903..6919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6927..6933,6934..6951,6952..6957))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6972..6993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7110..7127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7281..7869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7361..7380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8040..8831,8832..8900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8663..8682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8901..9005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9080..9099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc caccatggct tgggaagcga ggcgcgaacc cggcccccga agggccgccg
      781 tccgggagac ggtgatgctg ttgctgtgcc tgggggtccc gaccggccgc ccctacaacg
      841 tggacactga gagcgcgctg ctttaccagg gcccccacaa cacgctgttc ggctactcgg
      901 tcgtgctgca cagccacggg gcgaaccgat ggctcctagt gggtgcgccc actgccaact
      961 ggctcgccaa cgcttcagtg atcaatcccg gggcgattta cagatgcagg atcggaaaga
     1021 atcccggcca gacgtgcgaa cagctccagc tgggtagccc taatggagaa ccttgtggaa
     1081 agacttgttt ggaagagaga gacaatcagt ggttgggggt cacactttcc agacagccag
     1141 gagaaaatgg atccatcgtg acttgtgggc atagatggaa aaatatattt tacataaaga
     1201 atgaaaataa gctccccact ggtggttgct atggagtgcc ccctgattta cgaacagaac
     1261 tgagtaaaag aatagctccg tgttatcaag attatgtgaa aaaatttgga gaaaattttg
     1321 catcatgtca agctggaata tccagttttt acacaaagga tttaattgtg atgggggccc
     1381 caggatcatc ttactggact ggctctcttt ttgtctacaa tataactaca aataaataca
     1441 aggctttttt agacaaacaa aatcaagtaa aatttggaag ttatttagga tattcagtcg
     1501 gagctggtca ttttcggagc cagcatacta ccgaagtagt cggaggagct cctcaacatg
     1561 agcagattgg taaggcatat atattcagca ttgatgaaaa agaactaaat atcttacatg
     1621 aaatgaaagg taaaaagctt ggatcgtact ttggagcttc tgtctgtgct gtggacctca
     1681 atgcagatgg cttctcagat ctgctcgtgg gagcacccat gcagagcacc atcagagagg
     1741 aaggaagagt gtttgtgtac atcaactctg gctcgggagc agtaatgaat gcaatggaaa
     1801 caaacctcgt tggaagtgac aaatatgctg caagatttgg ggaatctata gttaatcttg
     1861 gcgacattga caatgatggc tttgaagatg ttgctatcgg agctccacaa gaagatgact
     1921 tgcaaggtgc tatttatatt tacaatggcc gtgcagatgg gatctcgtca accttctcac
     1981 agagaattga aggacttcag atcagcaaat cgttaagtat gtttggacag tctatatcag
     2041 gacaaattga tgcagataat aatggctatg tagatgtagc agttggtgct tttcggtctg
     2101 attctgctgt cttgctaagg acaagacctg tagtaattgt tgacgcttct ttaagccacc
     2161 ctgagtcagt aaatagaacg aaatttgact gtgttgaaaa tggatggcct tctgtgtgca
     2221 tagatctaac actttgtttc tcatataagg gcaaggaagt tccaggttac attgttttgt
     2281 tttataacat gagtttggat gtgaacagaa aggcagagtc tccaccaaga ttctatttct
     2341 cttctaatgg aacttctgac gtgattacag gaagcataca ggtgtccagc agagaagcta
     2401 actgtagaac acatcaagca tttatgcgga aagatgtgcg ggacatcctc accccaattc
     2461 agattgaagc tgcttaccac cttggtcctc atgtcatcag taaacgaagt acagaggaat
     2521 tcccaccact tcagccaatt cttcagcaga agaaagaaaa agacataatg aaaaaaacaa
     2581 taaactttgc aaggttttgt gcccatgaaa attgttctgc tgatttacag gtttctgcaa
     2641 agattgggtt tttgaagccc catgaaaata aaacatatct tgctgttggg agtatgaaga
     2701 cattgatgtt gaatgtgtcc ttgtttaatg ctggagatga tgcatatgaa acgactctac
     2761 atgtcaaact acccgtgggt ctttatttca ttaagatttt agagctggaa gagaagcaaa
     2821 taaactgtga agtcacagat aactctggcg tggtacaact tgactgcagt attggctata
     2881 tatatgtaga tcatctctca aggatagata ttagctttct cctggatgtg agctcactca
     2941 gcagagcgga agaggacctc agtatcacag tgcatgctac ctgtgaaaat gaagaggaaa
     3001 tggacaatct aaagcacagc agagtgactg tagcaatacc tttaaaatat gaggttaagc
     3061 tgactgttca tgggtttgta aacccaactt catttgtgta tggatcaaat gatgaaaatg
     3121 agcctgaaac gtgcatggtg gagaaaatga acttaacttt ccatgttatc aacactggca
     3181 atagtatggc tcccaatgtt agtgtggaaa taatggtacc aaattctttt agcccccaaa
     3241 ctgataagct gttcaacatt ttggatgtcc agactactac tggagaatgc cactttgaaa
     3301 attatcaaag agtgtgtgca ttagagcagc aaaagagtgc aatgcagacc ttgaaaggca
     3361 tagtccggtt cttgtccaag actgataaga ggctattgta ctgcataaaa gctgatccac
     3421 attgtttaaa tttcttgtgt aattttggga aaatggaaag tggaaaagaa gccagtgttc
     3481 atatccaact ggaaggccgg ccatccattt tagaaatgga tgagacttca gcactcaagt
     3541 ttgaaataag agcaacaggt tttccagagc caaatccaag agtaattgaa ctaaacaagg
     3601 atgagaatgt tgcgcatgtt ctactggaag gactacatca tcaaagaccc aaacgttatt
     3661 tcaccatagt gattatttca agtagcttgc tacttggact tattgtactt ctgttgatct
     3721 catatgttat gtggaaggct ggcttcttta aaagacaata caaatctatc ctacaagaag
     3781 aaaacagaag agacagttgg agttatatca acagtaaaag caatgatgat ggtacccttg
     3841 aggtgctttt ccaaggtccc ggttccggta gtgcatggtc tcatccccaa tttgaaaaag
     3901 gtggggggtc aggcggcggt tcaggcggga gtgcttggtc acacccccaa tttgagaaat
     3961 aatgatcact cgaggatatc gcggccgcga tctcgacaat caacctctgg attacaaaat
     4021 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4081 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4141 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4201 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4261 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4321 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4381 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4441 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4501 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4561 gatctccctt tgggccgcct ccccgcctgg aacgggggag gctaactgaa acacggaagg
     4621 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4681 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4741 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4801 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     4861 atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     4921 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4981 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5041 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5101 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5161 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5221 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5281 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     5341 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5401 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5461 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5521 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5581 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     5641 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     5701 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     5761 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5821 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5881 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     5941 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6001 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6061 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6121 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6181 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6241 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6301 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6361 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6421 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6481 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6541 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6601 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6661 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6721 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6781 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6841 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6901 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6961 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7021 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7081 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7141 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7201 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7261 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7321 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7381 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7441 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7501 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7561 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7621 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7681 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7741 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7801 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7861 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7921 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7981 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8041 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8101 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8161 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8221 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8281 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8341 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8401 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8461 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8521 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8581 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8641 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8701 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8761 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8821 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8881 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8941 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9001 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     9061 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     9121 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     9181 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag g
//
