LOCUS       sequence-444500-        9426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Marchantia polymorpha transformation vector carrying sulfadiazine 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Robinson K, Chia KS, Guyon A, Schornack S, Carella P
  TITLE     An Efficient Sulfadiazine Selection Scheme for Stable Transformation
            in the Model Liverwort Marchantia polymorpha.
  JOURNAL   J Exp Bot. 2024 Jun 2:erae256. doi: 10.1093/jxb/erae256.
  PUBMED    38824404
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2024 Jun 2:erae256. doi: 10.1093/jxb/erae256."
COMMENT     Sequence Label: pMpGWBs00
FEATURES             Location/Qualifiers
     source          1..9426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      362..614
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(647..663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(647..663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(660..682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    671..687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(695..701,702..719,720..725))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    740..761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    846..1020
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2077..2753)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2120..2140)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    2948..2972
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3493..4284
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4530..5118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5019..5038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5272..5289
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5304..5444
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5370..5389)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5530..5552
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5788..5982
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6048..7121)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7550..8179)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaatttccc
      361 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
      421 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
      481 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
      541 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
      601 tatgttacta gatcgggaat tggttccgga accaattcgt aatcatgtca tagctgtttc
      661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      721 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt aggcgcgcct
      781 atttctgaat tcaacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
      841 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
      901 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
      961 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     1021 ctcgagctag gcatgatcta accctcggtc tctggcgtcg cgactgcgaa atttcgcgag
     1081 ggtttccgag atggtgattg cgcttcgcag atctccaggc gcgtgggtgc ggacgtagtc
     1141 agcgccattg ccgatcgcgt gaagttccgc cgcaaggctc gctggaccca gatcctttac
     1201 aggaaggcca acggtggcgc ccaagaagga tttccgcgac accgagacca atagcggaag
     1261 ccccaacgcc gacttcagct tttgaaggtt cgacagcacg tgcagcgatg tttccggtgc
     1321 ggggctcaag aaaaatccca tccccggatc gaggatgagc cggtcggcag cgaccccgct
     1381 ccgtcgcaag gcggaaaccc gcgcctcgaa gaaccgcaca atctcgtcga gcgcgtcttc
     1441 gggtcgaagg tgaccggtgc gggtggcgat gccatcccgc tgcgctgagt gcataaccac
     1501 cagcctgcag tccgcctcag caatatcggg atagagcgca gggtcaggaa atccttggat
     1561 atcgttcagg tagcccacgc cgcgcttgag cgcatagcgc tgggtttccg gttggaagct
     1621 gtcgattgaa acacggtgca tctgatcgga cagggcgtct aagagcggcg caatacgtct
     1681 gatctcatcg gccggcgata caggcctcgc gtccggatgg ctggcggccg gtccgacatc
     1741 cacgacgtct gatccgactc gcagcatttc gatcgccgcg gtgacagcgc cggcggggtc
     1801 tagccgccgg ctctcatcga agaaggagtc ctcggtgaga ttcagaatgc cgaacaccgt
     1861 caccatgcac tttactcttc caccattgct tgtaatggaa gtaatgtcag tgttgacctt
     1921 cttaactggg aatccagtca tggatttgag gccgccgaat ggagccaccg cggccgattg
     1981 caccgtagaa gcacggctga ctgtagtcac agctgaagag gatatcatag aagccatctc
     2041 gaggagagat agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa
     2101 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     2161 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     2221 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     2281 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     2341 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     2401 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     2461 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct
     2521 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     2581 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     2641 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     2701 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     2761 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     2821 ccatgttggc aagctgctct agccaatacg caaaccgaat tcagaaataa attcagccta
     2881 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     2941 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     3001 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     3061 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     3121 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     3181 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     3241 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     3301 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     3361 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     3421 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     3481 aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     3541 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     3601 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     3661 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     3721 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     3781 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     3841 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     3901 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     3961 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     4021 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     4081 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     4141 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     4201 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     4261 atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt
     4321 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     4381 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     4441 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4921 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5161 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5221 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5281 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     5341 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5401 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     5461 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5521 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5581 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     5641 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     5701 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     5761 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     5821 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     5881 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     5941 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     6001 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     6061 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     6121 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     6181 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     6241 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     6301 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     6361 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
     6421 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
     6481 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
     6541 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
     6601 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
     6661 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
     6721 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
     6781 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
     6841 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
     6901 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
     6961 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
     7021 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
     7081 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
     7141 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
     7201 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     7261 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
     7321 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
     7381 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
     7441 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
     7501 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
     7561 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
     7621 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
     7681 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
     7741 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
     7801 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
     7861 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
     7921 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
     7981 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
     8041 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
     8101 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
     8161 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
     8221 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
     8281 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
     8341 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
     8401 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
     8461 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
     8521 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
     8581 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
     8641 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
     8701 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
     8761 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
     8821 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
     8881 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
     8941 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
     9001 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
     9061 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
     9121 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
     9181 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
     9241 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
     9301 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
     9361 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
     9421 aaacct
//
