LOCUS       sequence-444503-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All serines in the LC domain to glycine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 StoG
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcgggtaa cgactacacc cagcaagcga cccaaggtta tggtgcgtat ccgacccagc
      121 cgggtcaagg ttatggtcag caaggtggcc agccgtatgg tcagcaaggc tacggtggct
      181 atggtcaggg caccgatacc ggtggctacg gtcagggtgg ctacggtggc tatggccagg
      241 gccaaaacac cggttatggc acccaaggta ccccgcaagg ttatggtggc accggtggct
      301 atggtggcgg tcagggcggt caaggcggtt acggtcagca aggcggttac ccgggttatg
      361 gtcaacaacc ggcgccgggc ggtaccggcg gtggctacgg tggcggtggc cagggtggcg
      421 gttatggtca gccgcaaggc ggtggctacg gtcagcaacc gggctatggt ggccagcaac
      481 agggttacgg ccaacagcaa ggttataacc cgccgcaggg ttacggccag caaaaccaat
      541 ataacggtag cagcggtggc ggtggcggtg gcggtggcgg tggcaactac ggtcaggacc
      601 aaagcagcat gagcagcggt ggcggtagcg gcggtggcta cggtaaccaa gaccaaagcg
      661 gtggcggtgg cagcggtggc tatggtcagc aagatcgtgg tggccgtggt cgtggtggca
      721 gcggtggcgg tggcggtggc ggtggcggtg gctacaaccg tagcagcggt ggctatgaac
      781 cgcgtggtcg tggtggcggt cgtggcggtc gtggcggtat gggcggtagc gatcgtggcg
      841 gtttcaacaa gtttggcggt ccgcgtgacc agggtagccg tcacgatagc gaacaagaca
      901 acagcgataa ctgactcgag
//
