LOCUS       sequence-444506-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All serines to glycines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC StoG
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcgggtaa cgactacacc cagcaagcga cccaaggtta tggtgcgtat ccgacccagc
      121 cgggtcaagg ttatggtcag caaggtggcc agccgtatgg tcagcaaggc tacggtggct
      181 atggtcaggg caccgatacc ggtggctacg gtcagggtgg ctacggtggc tatggccagg
      241 gccaaaacac cggttatggc acccaaggta ccccgcaagg ttatggtggc accggtggct
      301 atggtggcgg tcagggcggt caaggcggtt acggtcagca aggcggttac ccgggttatg
      361 gtcaacaacc ggcgccgggc ggtaccggcg gtggctacgg tggcggtggc cagggtggcg
      421 gttatggtca gccgcaaggc ggtggctacg gtcagcaacc gggctatggt ggccagcaac
      481 agggttacgg ccaacagcaa ggttataacc cgccgcaggg ttacggccag caaaaccaat
      541 ataacggttg actcgag
//
