LOCUS       sequence-444513-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All glutamines in the LC domain to serine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 QtoS
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(727..750)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggctagtaa tgattatact tcatctgcaa caagctccta tggcgcgtat ccgaccagcc
      121 cgggtagcgg ttatagcagt tcgagctcct ctccttatgg ttcttcgagc tacagcggct
      181 acagctccag cacggatacc agcggttacg gctcctcgtc ttacagctcc tatggcagct
      241 cctctaacac cggttacggc accagctcta ctccgagcgg ttatggttct acgggtggct
      301 acggcagttc ctcttcttct tccagcagct acggctcctc atcgtcttac ccgggttacg
      361 gttccagccc ggctccgagc tcaaccagcg gcagctacgg tagctctagc agcagctcgt
      421 cgtacggctc cccgagctcg ggcagctata gcagttcccc gtcatacggc ggcagcagca
      481 gctcgtatgg cagcagctct tcgtacaacc cgccaagcgg ttatggctca tccaatagct
      541 ataacagctc ctccgggggt ggcggtggtg gcggtggcgg cgggaactat ggccaagacc
      601 agagcagcat gagcagcggc ggtggtagcg gtgggggcta cggcaatcag gaccaaagcg
      661 gtggcggtgg cagcggtggt tacggccagc aggaccgcgg tggccgtggt cgtggcggat
      721 ccggcggtgg tggtggtggt ggtggtggtg gctataatcg tagctccggt ggctacgagc
      781 cgcgtggtcg cggtggtggc agaggtggac gcggtggcat gggtggctcg gatcgtggtg
      841 gttttaacaa gttcggtggc ccacgtgatc aaggttctcg tcacgatagc gaacaggaca
      901 acagcgacaa ttgactcgag
//
