LOCUS       sequence-444514-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All glutamines in the LC domain to asparagine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 QtoN
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcttcaaa tgattataca aataacgcaa ctaactcgta tggtgcgtat ccgaccaacc
      121 cgggtaacgg ctacagcaat aacagctcta atccgtatgg caacaactcg tacagcggtt
      181 attccaacag caccgatacc agcggttacg gtaacagcag ctatagcagc tacggcaaca
      241 gcaataatac cggttacggc accaactcta ctccgaacgg ttacggttcc acgggtggct
      301 atggtagcag caacagctcc aattcgtcat acggtaacaa ctcttcgtac ccgggttacg
      361 gtaataaccc ggctccgtcc tctacgagcg gctcctacgg tagctcttct aatagctcct
      421 catatggcaa cccgaattcc ggctcctata gtaacaatcc gagctacggt ggcaacaaca
      481 actcctacgg caataacaac tcctataacc cgcctaatgg ttacggtaac aataacaatt
      541 ataacagcag cagcggtggc ggcggcggtg ggggtggtgg cgggaactat ggccaagacc
      601 aaagcagcat gagctctggt ggcggctccg gaggcggcta cggtaaccag gaccagagcg
      661 gaggcggtgg tagcggtggc tatggccagc aggatcgtgg tggccgcggt agaggtggtt
      721 ctggcggagg cggcggaggg ggtggtggcg gctacaatcg tagctcaggt ggttacgagc
      781 cgcgtggtcg cggcggtggt cgtggtggtc gcggcggtat gggtggctcg gaccgtggcg
      841 gcttcaataa gtttggtggc ccacgtgatc aaggttctcg tcacgatagt gaacaggaca
      901 atagcgacaa ctgactcgag
//
