LOCUS       sequence-444516-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All glutamines in the LC domain to alanine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 QtoA
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcttcaaa tgattatact gcggctgcaa cagcgtccta tggcgcgtat ccgaccgcac
      121 cgggtgctgg ctatagcgct gcctcctctg caccgtatgg cgctgcgagc tactctggtt
      181 acagcgcgtc gaccgatacc agcggttatg gtgcctcctc ctacagcagc tacggcgcga
      241 gtgctaatac cggttatggt acggcatcca ctccggcagg ctatggctct accggtggtt
      301 acggtagctc ggcgagcagc gccagctcat acggcgcagc gtcttcgtac ccgggttatg
      361 gtgccgctcc ggcaccgagc tctacgagcg gtagctacgg ttctagctca gcgtccagct
      421 cgtacggcgc gccggcttcc ggttcgtact ccgcggcgcc aagctacggc ggtgcggctg
      481 ccagctatgg cgcggcggcg tcttataacc cgcctgccgg ttacggcgcg gcgaatgcat
      541 ataacagcag ctctggcggc ggtggtggtg gaggcggcgg cggcaattat ggtcaggacc
      601 aaagcagcat gagctccggc ggtggtagcg gtgggggcta cggcaaccag gaccagagcg
      661 gcggaggcgg cagcggtggc tacggccaac aggaccgcgg tggccgtggt cgcggcggct
      721 ccggtggtgg cggtggcggc ggtggcggcg ggtacaatcg tagcagcggt ggttacgagc
      781 cgcgtggacg cggtggcggt cgtggtggta gaggcggtat gggtggctca gaccgtggtg
      841 gttttaacaa gttcggcgga ccgcgtgatc aaggttctcg tcacgatagc gaacaggaca
      901 acagcgataa ctgactcgag
//
