LOCUS       sequence-444518-       12466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-on lentiviral vector for controlled expression of a secretable 
            and permeable form of C. botulinum exoenzyme C3 transferase fused 
            with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Gutekunst CA, Tung JK, McDougal ME, Gross RE
  TITLE     C3 transferase gene therapy for continuous conditional RhoA 
            inhibition.
  JOURNAL   Neuroscience. 2016 Dec 17;339:308-318. doi: 
            10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13.
  PUBMED    27746349
REFERENCE   2  (bases 1 to 12466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2016-12-17"; volume: "339:308-318. doi"; 
            pages: " 10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13"
COMMENT     Sequence Label: pLVUT-spC3GFP
FEATURES             Location/Qualifiers
     source          1..12466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        233..612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..812
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     767..787
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             830..1010
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2435..2552
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2617..3828
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3665..3684
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     sig_peptide     3875..3934
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     regulatory      4835..4844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4841..4843,4844..4846,4847..5560)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4841..4843,4844..4846,4847..5557)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4886..4907)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5147..5166)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5494..5515
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5581..6148
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5599..6150
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5743..5760)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5970..5989
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6153..6773
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(6226..6245)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             6774..6794
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6897..7091
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     misc_feature    7232..7820
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7285..7305)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7703..7714)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7823..7839)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7824..7840)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    join(7960..7978,7979..8001,8002..8020,8021..8043,8044..8062,
                     8063..8084,8085..8103,8104..8126,8127..8145,8146..8168,
                     8169..8187,8188..8210,8211..8229)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             8268..8448
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8472..8489)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8478..8702
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8748..9176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8835..8854)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9045..9066
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9185..9205)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9190..9519
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9370..9505
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9432..9451
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9567..9614
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9633..10007
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10137..10258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10174..10193)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10228..10247
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10307..10323)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10307..10323)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10320..10342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10331..10347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10355..10361,10362..10379,10380..10385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10400..10421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10538..10555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10709..11297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10789..10808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11468..12259,12260..12328))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12091..12110
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12329..12433)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      241 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      301 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      361 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      421 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      481 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      541 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      601 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      661 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      721 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      781 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
      841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1021 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2401 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2461 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2521 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2581 cagtttggtt aattaacccg tgtcggctcc agatctggcc tccgcgccgg gttttggcgc
     2641 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     2701 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     2761 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     2821 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     2881 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     2941 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     3001 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     3061 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     3121 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     3181 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     3241 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     3301 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     3361 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     3421 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     3481 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     3541 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     3601 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     3661 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     3721 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     3781 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttgggct
     3841 gcaggtcgac tctagaggat ccccgggtac cggtatggag acagacacac tcctgctatg
     3901 ggtactgctg ctctgggttc caggttccac tggtgacgcg gcccagccgg ccaggcgcgc
     3961 cgtacgaagc ttggtaccga gctcggatcc actccagtgt ggtggaattc tgcagatatc
     4021 gatgggagga tacggccgaa agaagcgacg acagcgacgc cgtggaggag gcggtaccat
     4081 ggaccagaag ctgatctccg aggaggacct gggatcctct agagtcgacc tgcaggcatg
     4141 caatgcttat tccattaatc aaaaggctta ttcaaatact taccaggagt ttactaatat
     4201 tgatcaagca aaagcttggg gtaatgctca gtataaaaag tatggactaa gcaaatcaga
     4261 aaaagaagct atagtatcat atactaaaag cgctagtgaa ataaatggaa agctaagaca
     4321 aaataaggga gttatcaatg gatttccttc aaatttaata aaacaagttg aacttttaga
     4381 taaatctttt aataaaatga agacccctga aaatattatg ttatttagag gcgacgaccc
     4441 tgcttattta ggaacagaat ttcaaaacac tcttcttaat tcaaatggta caattaataa
     4501 aacggctttt gaaaaggcta aagctaagtt tttaaatgaa gatagacttg aatatggata
     4561 tattagtact tcgttaatga atgtttctca atttgcagga agaccaatta ttacaaaatt
     4621 taaagtagca aaaggctcaa aggcaggata tattgaccct attagtgctt ttgcaggaca
     4681 acttgaaatg ttgcttccta gacatagtac ttatcatata gacgatatga gattatcttc
     4741 tgatggtaaa caaataataa ttacagcaac aatgatgggc acagctatca atcctaaagg
     4801 gcccgggatc caccggccgg tcgccacacc ggtcgccacc atggtgagca agggcgagga
     4861 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4921 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4981 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5041 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5101 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5161 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5221 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5281 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5341 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5461 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5521 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagaat
     5581 tccgcccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5641 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5701 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5761 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5821 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5881 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5941 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     6001 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     6061 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     6121 ggttttcctt tgaaaaacac gatgataata ccatggctag attagataaa agtaaagtga
     6181 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     6241 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
     6301 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
     6361 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
     6421 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
     6481 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
     6541 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
     6601 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
     6661 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
     6721 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcgccaaaaa
     6781 agaagagaaa ggtcgacggc ggtggtgctt tgtctcctca gcactctgct gtcactcaag
     6841 gaagtatcat caagaacaag gagggcatgg atgctaagtc actaactgcc tggtcccgga
     6901 cactggtgac cttcaaggat gtatttgtgg acttcaccag ggaggagtgg aagctgctgg
     6961 acactgctca gcagatcgtg tacagaaatg tgatgctgga gaactataag aacctggttt
     7021 ccttgggtta tcagcttact aagccagatg tgatcctccg gttggagaag ggagaagagc
     7081 cctggctggt ggagagagaa attcaccaag agacccatcc tgattcagag actgcatttg
     7141 aaatcaaatc atcagtttaa ggatctcgac tagctagtag ctagctagct agtcgagctc
     7201 aagcttcgaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     7261 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7321 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7381 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7441 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7501 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7561 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7621 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7681 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7741 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7801 cctttgggcc gcctccccgc atcgataccg tcgacctcga gtacctttaa gaccaatgac
     7861 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7921 aattcactcc caacgaagac aagatctcga gtttaccact ccctatcagt gatagagaaa
     7981 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     8041 cactccctat cagtgataga gaaaagtgaa agtgagttta ccactcccta tcagtgatag
     8101 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     8161 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     8221 tgatagagaa aagtgaaagt cggatcgatc tgctttttgc ttgtactggg tctctctggt
     8281 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8341 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8401 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaaac
     8461 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8521 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     8581 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     8641 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     8701 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     8761 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8821 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8881 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8941 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9001 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9061 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9121 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     9181 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     9241 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9301 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     9361 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9421 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     9481 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     9541 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     9601 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     9661 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     9721 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     9781 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     9841 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     9901 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     9961 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
    10021 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    10081 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    10141 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    10201 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10261 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    10321 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    10381 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    10441 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    10501 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    10561 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    10621 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10681 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10741 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10801 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10861 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10921 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10981 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    11041 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    11101 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    11161 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    11221 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    11281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    11341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    11401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    11461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    11521 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    11581 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    11641 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    11701 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    11761 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    11821 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11881 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11941 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    12001 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    12061 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    12121 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    12181 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    12241 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    12301 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    12361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    12421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcga
//
