LOCUS       sequence-444519-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All threonines in the LC domain to valine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 TtoV
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcgagcaa cgattacgtt cagcaagcgg tgcagagcta tggtgcgtac ccggttcaac
      121 cgggtcaggg ttacagccaa cagagcagcc aaccgtatgg tcaacagagc tatagcggtt
      181 acagccaaag cgtggacgtt agcggttatg gtcaaagcag ctacagcagc tatggtcaga
      241 gccaaaacgt tggctacggt gttcagagcg ttccgcaggg ctacggtagc gtgggtggtt
      301 atggtagcag ccaaagcagc cagagcagct acggtcagca aagcagctac ccgggttatg
      361 gccagcaacc ggcgccgagc agcgttagcg gtagctacgg tagcagcagc cagagcagca
      421 gctatggtca accgcaaagc ggcagctata gccaacagcc gagctatggc ggtcagcaac
      481 agagctacgg tcaacagcaa agctataacc cgccgcaggg ttatggccaa cagaaccagt
      541 acaacagcag cagcggtggc ggtggcggtg gtggcggcgg tggtaactac ggtcaggacc
      601 aaagcagcat gagcagcggc ggtggtagcg gtggtggtta cggtaaccag gatcaaagcg
      661 gcggtggcgg tagcggtggc tacggtcaac aggatcgtgg tggccgtggt cgtggtggca
      721 gcggcggcgg tggtggtggt ggcggcggtg gctacaaccg tagcagcggt ggttatgaac
      781 cgcgtggtcg tggcggtggt cgtggtggtc gtggcggtat gggcggtagc gaccgtggcg
      841 gttttaacaa gttcggcggt ccgcgtgatc agggtagccg tcacgacagc gagcaggaca
      901 acagcgacaa ctgactcgag
//
