LOCUS       sequence-444520-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All threonines in the LC domain to serine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 TtoS
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcgagcaa cgattatagc cagcaagcga gccagagcta cggtgcgtat ccgagccaac
      121 cgggccaggg ttacagccag caaagcagcc agccgtatgg tcagcagagc tacagcggct
      181 atagccagag cagcgacagc agcggttatg gccagagcag ctacagcagc tatggtcaaa
      241 gccagaacag cggttacggt agccaaagca gcccgcaggg ctatggtagc agcggtggct
      301 acggtagcag ccagagcagc caaagcagct acggccagca aagcagctac ccgggttatg
      361 gtcagcagcc ggcgccgagc agcagcagcg gtagctatgg cagcagcagc caaagcagca
      421 gctacggtca gccgcaaagc ggtagctaca gccagcaacc gagctacggt ggtcaacagc
      481 aaagctacgg tcaacagcag agctataacc cgccgcaagg ttatggccaa cagaaccagt
      541 acaacagcag cagcggcggc ggtggtggtg gcggtggcgg cggtaactac ggtcaagacc
      601 agagcagcat gagcagcggt ggtggcagcg gtggcggcta tggtaaccag gatcaaagcg
      661 gtggcggtgg tagcggtggc tatggtcagc aagaccgtgg tggccgtggc cgtggtggta
      721 gcggcggtgg cggcggtggc ggtggtggcg gttacaaccg tagcagcggc ggttatgaac
      781 cgcgtggtcg tggcggtggt cgtggtggcc gtggtggcat gggcggtagc gatcgtggtg
      841 gcttcaacaa atttggtggc ccgcgtgatc aaggcagccg tcacgacagc gaacaggata
      901 acagcgacaa ctgactcgag
//
