LOCUS       sequence-444522-       11668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-on lentiviral vector for controlled expression of secretable and
            permeable EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Gutekunst CA, Tung JK, McDougal ME, Gross RE
  TITLE     C3 transferase gene therapy for continuous conditional RhoA 
            inhibition.
  JOURNAL   Neuroscience. 2016 Dec 17;339:308-318. doi: 
            10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13.
  PUBMED    27746349
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2016-12-17"; volume: "339:308-318. doi"; 
            pages: " 10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13"
COMMENT     Sequence Label: pLVUT-spGFP
FEATURES             Location/Qualifiers
     source          1..11668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        233..612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..812
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     767..787
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             830..1010
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2435..2552
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2617..3828
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3665..3684
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     sig_peptide     3875..3934
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             join(4040..4042,4043..4045,4046..4759)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4040..4042,4043..4045,4046..4756)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4085..4106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4346..4365)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4693..4714
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4780..5349
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4800..5351
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4944..4961)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5171..5190
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5354..5974
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5427..5446)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             5975..5995
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6098..6292
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     misc_feature    6433..7021
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6486..6506)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6904..6915)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7024..7040)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7025..7041)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    join(7161..7179,7180..7202,7203..7221,7222..7244,7245..7263,
                     7264..7286,7287..7305,7306..7328,7329..7347,7348..7370,
                     7371..7389,7390..7412,7413..7431)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             7470..7650
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7674..7691)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7680..7904
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7950..8378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8037..8056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8247..8268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8387..8407)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8392..8721
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8572..8707
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8634..8653
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8769..8816
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8835..9209
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9339..9460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9376..9395)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9430..9449
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9509..9525)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9509..9525)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9522..9544)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9533..9549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9557..9563,9564..9581,9582..9587))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9602..9623
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9740..9757)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9911..10499)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9991..10010)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10670..11461,11462..11530))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11293..11312
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11531..11635)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      241 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      301 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      361 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      421 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      481 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      541 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      601 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      661 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      721 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      781 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
      841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1021 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2401 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2461 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2521 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcaaagatc
     2581 cagtttggtt aattaacccg tgtcggctcc agatctggcc tccgcgccgg gttttggcgc
     2641 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     2701 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     2761 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     2821 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     2881 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     2941 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     3001 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     3061 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     3121 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     3181 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     3241 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     3301 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     3361 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     3421 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     3481 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     3541 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     3601 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     3661 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     3721 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     3781 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttgggct
     3841 gcaggtcgac tctagaggat ccccgggtac cggtatggag acagacacac tcctgctatg
     3901 ggtactgctg ctctgggttc caggttccac tggtgacgcg gcccagccgg ccaggcgcgc
     3961 cgtacgaagc ttggtaccaa tgggaggata cggccgaaag aagcgacgac agcgacgccg
     4021 tggaggaggc aggatccgaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4081 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4141 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4201 gcccgtgccc tggcccaccc tcgtgaccac cctgacctat ggcgtgcagt gcttcagccg
     4261 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4321 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4381 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4441 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4501 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4561 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4621 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     4681 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4741 ggacgagctg tacaagtaaa gcggccgcga ctctagaatt ccgccccccc ccccccntaa
     4801 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     4861 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     4921 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     4981 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5041 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5101 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5161 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     5221 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     5281 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     5341 cgatgataat accatggcta gattagataa aagtaaagtg attaacagcg cattagagct
     5401 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt
     5461 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc
     5521 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca
     5581 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
     5641 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
     5701 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc
     5761 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga
     5821 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt
     5881 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg
     5941 attagaaaaa caacttaaat gtgaaagtgg gtcgccaaaa aagaagagaa aggtcgacgg
     6001 cggtggtgct ttgtctcctc agcactctgc tgtcactcaa ggaagtatca tcaagaacaa
     6061 ggagggcatg gatgctaagt cactaactgc ctggtcccgg acactggtga ccttcaagga
     6121 tgtatttgtg gacttcacca gggaggagtg gaagctgctg gacactgctc agcagatcgt
     6181 gtacagaaat gtgatgctgg agaactataa gaacctggtt tccttgggtt atcagcttac
     6241 taagccagat gtgatcctcc ggttggagaa gggagaagag ccctggctgg tggagagaga
     6301 aattcaccaa gagacccatc ctgattcaga gactgcattt gaaatcaaat catcagttta
     6361 aggatctcga ctagctagta gctagctagc tagtcgagct caagcttcga attcgatatc
     6421 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6481 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6541 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6601 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6661 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6721 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6781 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6841 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6901 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6961 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7021 catcgatacc gtcgacctcg agtaccttta agaccaatga cttacaaggc agctgtagat
     7081 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7141 caagatctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     7201 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     7261 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     7321 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     7381 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     7441 gtcggatcga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7501 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7561 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7621 ccttttagtc agtgtggaaa atctctagca gcatctagaa acccgctgat cagcctcgac
     7681 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7741 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     7801 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7861 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     7921 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     7981 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8041 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8101 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8161 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8221 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8281 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8341 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     8401 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8461 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8521 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8581 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8641 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     8701 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     8761 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     8821 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     8881 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     8941 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     9001 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     9061 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     9121 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     9181 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     9241 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     9301 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     9361 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9421 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     9481 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9721 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9781 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9841 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10321 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10621 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10681 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10741 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10801 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10861 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10921 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10981 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11041 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11101 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11161 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11221 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11281 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11341 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11401 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11461 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11521 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11581 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11641 ttccccgaaa agtgccacct gacgtcga
//
