LOCUS       sequence-444527-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  4 glutamine pairs to alanine pairs at positions 27, 28, 92, 93, 132,
            133, 157, 158.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC 4QQ->AA
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcttcaaa tgattataca cagcaagcaa ctcagtcgta cggcgcgtat ccgacccagc
      121 cgggtcaggg ttatagcgcg gccagctctc aaccgtatgg ccaacaatcc tacagcggtt
      181 acagccagag taccgacacc agcggttatg gtcagagctc ctatagctcc tacggccaat
      241 cccaaaacac cggttatggc acccagtcta ctccgcaggg ttacggttcc acgggtggtt
      301 acggcagctc ccaaagctct caaagcagct atggcgcggc gagctcttac ccgggttatg
      361 gccagcagcc ggcaccgtct agcacgagcg gctcctacgg gtcgtcgtcg caaagctcca
      421 gctacggcca gccacagtct ggcagctaca gcgcagctcc gagctacggc ggtcaacaac
      481 agtcttatgg tcaacagcag tcatacaacc cgcctcaagg ttacggcgcg gctaatcagt
      541 ataacagctg actcgag
//
