LOCUS       sequence-444528-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  4 glutamine pairs to asparagine pairs at positions 27, 28, 92, 93, 
            132, 133, 157, 158.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC 4QQ->NN
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcttcaaa tgattataca cagcaagcaa ctcaaagcta cggcgcgtat ccgacccagc
      121 cgggtcaagg ttatagtaat aacagctcgc aaccgtatgg ccagcagagc tacagcggtt
      181 atagccagtc tactgacacc agcggttatg gtcaatcctc atacagctcc tacggccagt
      241 cccagaatac cggttacggc acccagtcca ccccgcaggg ttatggtagc acgggtggtt
      301 atggcagcag ccagtcctcc caatcttcgt acggcaacaa cagctcctac ccgggttatg
      361 gccaacaacc ggctccgagc tctacgagcg gtagctacgg cagctcttcg caatccagct
      421 cttacggcca acctcagtct ggtagctaca gcaataaccc gagctacggc ggtcagcaac
      481 aatcgtacgg ccagcaacag agctacaatc caccgcaggg ctatggtaac aacaaccagt
      541 ataacagctg actcgag
//
