LOCUS       sequence-444529-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  12 serines to glycines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC 12S->G
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcttcaaa tgattataca cagcaagcaa ctcaatctta cggtgcgtat ccgacccagc
      121 caggtcaggg ctatagccag cagtctggtc agccgtatgg tcaacaaagc tatagcggct
      181 actcccaggg cactgacacc ggtggctacg gccagggctc ttacagcagc tacggccaaa
      241 gccaaaacac cggttatggg acgcagggta cgccgcaggg ctatggctcc accggcggtt
      301 atggctcagg tcaatcgagc cagggtagct atggccaaca aggttcctac ccgggttacg
      361 gccaacaacc ggctccgagt ggcaccagcg gttcctacgg tagcagcggt cagggttcga
      421 gctacggtca gccgcagggt ggttcctaca gccagcaacc gagctacggc ggtcaacagc
      481 aatcctacgg ccagcaacaa tcttacaatc cgcctcaggg ttatggtcag cagaaccagt
      541 ataacagctg actcgag
//
