LOCUS       sequence-444530-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  12 serines to alanines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC 12S->A
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcttcaaa tgattatact caacaggcaa cacagagcta cggcgcatat ccgacgcaac
      121 caggtcaggg ctattctcag cagtctgcgc agccgtatgg ccagcagtcc tactccggtt
      181 attcccaagc cactgacacc gcgggttatg gccaagcgag ctacagcagc tacggccaaa
      241 gccagaatac cggttatggc acccaggcga ccccgcaggg ttatggttcg accggtggtt
      301 acggctccgc gcagtcgagc caagcgagct acggccaaca agctagctac ccgggttacg
      361 gccagcaacc ggcaccgagc gcgacgagcg gtagctacgg ctcttcagcc caagcttcga
      421 gctacggcca gccgcaggct ggtagctatt ctcagcaacc gagctacgga ggtcaacaac
      481 aatcctacgg ccagcagcag agttacaacc cgcctcaggg ttatggtcaa caaaaccagt
      541 ataacagctg actcgag
//
