LOCUS       sequence-444539-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7S / Q160S double mutation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC noYQ_noYT
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcgagcaa cgactacagc cagcaagcga cccagagcta cggtgcgtat ccgacccagc
      121 cgggtcaagg ctacagccag caaagcagcc agccgtatgg tcagcaaagc tacagcggct
      181 atagccagag caccgatacc agcggttacg gccaaagcag ctacagcagc tatggccaga
      241 gccaaaacac cggttatggc acccagagca ccccgcaagg ttacggcagc accggtggct
      301 atggtagcag ccagagcagc caaagcagct acggccagca aagcagctat ccgggttatg
      361 gtcaacagcc ggcgccgagc agcaccagcg gtagctacgg tagcagcagc cagagcagca
      421 gctacggtca gccgcaaagc ggcagctaca gccagcaacc gagctatggt ggccagcaac
      481 agagctacgg tcaacagcaa agctataacc cgccgcaggg ttacggccag caaaacagct
      541 ataacagcta actcgag
//
