LOCUS       sequence-44454-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Isakoff SJ, Engelman JA, Irie HY, Luo J, Brachmann SM, Pearline RV, 
            Cantley LC, Brugge JS
  TITLE     Breast cancer-associated PIK3CA mutations are oncogenic in mammary 
            epithelial cells.
  JOURNAL   Cancer Res. 2005 Dec 1. 65(23):10992-1000.
  PUBMED    16322248
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2005 Dec 1. 65(23):10992-1000."
COMMENT     Sequence Label: JP1520 PIK3CA WT HA
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      769..778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             845..871
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             845..871
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 attactcttt cagaacaatg agatcatctt taaaaatggg gatgatttac ggcaagatat
       61 gctaaccctt cagattattc gcattatgga aaatatctgg caaaatcaag gtcttgatct
      121 tcgaatgtta ccttatggat gtctgtcaat cggtgactgt gtgggactta tcgaggtggt
      181 gagaaattct cacactataa tgcagattca gtgtaaagga ggcctgaaag gtgcactgca
      241 gtttaacagc cacacactcc atcagtggct caaagacaag aacaaggggg aaatatatga
      301 tgcggccatc gatttgttta cacgatcatg tgctggatat tgtgttgcca ccttcatttt
      361 gggaattgga gatcgtcaca atagtaatat catggttaaa gatgatggac aactgtttca
      421 tatagatttt ggacactttt tggatcacaa gaagaaaaaa tttggttata aacgagagcg
      481 cgtgccgttt gttttgacac aagatttctt aatagtgatt agtaaaggag cccaagaatg
      541 cacaaagaca agagaatttg agaggtttca ggagatgtgt tacaaggctt atctagctat
      601 tcggcagcat gccaatctct tcataaatct tttctcaatg atgcttggct ctggaatgcc
      661 agaactgcaa tcttttgatg atattgcata cattcgaaag accctagctt tagataaaac
      721 tgagcaagag gctttggagt atttcatgaa acaaatgaat gatgcacacc atggtggctg
      781 gacaacaaaa atggattgga tcttccacac aattaagcag catgctttga acctcgagat
      841 ggcctacccc tacgacgtgc ccgactacgc ctcctgatct agagggccca ggtaagtggt
      901 cataatcata atcataatca taatcataat
//
