LOCUS       sequence-444542-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  12 serines to asparagines .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC 12S->N
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcttcaaa tgattataca cagcaagcaa ctcaatctta cggcgcgtat ccgacccagc
      121 cgggtcaggg ttatagccag caatcaaatc agccgtatgg ccaacaaagc tattctggtt
      181 atagccaaaa cacggacacc aatggttatg gccaaaacag ctacagcagc tatggtcagt
      241 ctcagaacac cggttacggc acccagaaca ctccgcaggg ctacggctcc accggtggtt
      301 atggtagcaa tcagagctcc caaaacagtt acggtcagca aaacagctac ccgggttacg
      361 gccaacagcc ggctccgtcc aacacgagcg gttcttacgg ctccagcaat cagaattcca
      421 gctacggcca gccacagaac ggttcctaca gccagcagcc gagctatggt ggtcaacaac
      481 aatcgtacgg ccagcaacaa tcgtacaatc cgcctcaggg ctacggccag caaaaccagt
      541 ataactcgtg actcgag
//
