LOCUS       sequence-444546-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All glutamines to alanine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC QtoA
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggtag cgacaagatt caccaccacc accaccacga gaacctgtat ttccagggta
       61 tggcgagcaa cgattacacc gcggcggcga ccgcgagcta tggtgcgtat ccgaccgcgc
      121 cgggtgcggg ttacagcgcg gcgagcagcg cgccgtatgg tgcggcgagc tacagcggct
      181 atagcgcgag caccgacacc agcggttatg gtgcgagcag ctacagcagc tatggcgcga
      241 gcgcgaacac cggttatggt accgcgagca ccccggcggg ttatggtagc accggtggct
      301 atggtagcag cgcgagcagc gcgagcagct atggtgcggc gagcagctac ccgggttatg
      361 gtgcggcgcc ggcgccgagc agcaccagcg gtagctacgg tagcagcagc gcgagcagca
      421 gctatggtgc gccggcgagc ggtagctaca gcgcggcgcc gagctatggt ggcgcggcgg
      481 cgagctacgg tgcggcggcg agctacaatc cgccggcggg ctatggtgcg gcgaatgcgt
      541 ataacagcta actcgag
//
