LOCUS       sequence-44464-e       11222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11222)
  AUTHORS   Chandler KJ, Chandler RL, Mortlock DP
  TITLE     Identification of an ancient Bmp4 mesoderm enhancer located 46 kb 
            from the promoter.
  JOURNAL   Dev Biol. 2009 Mar 15;327(2):590-602. Epub 2009 Jan 3.
  PUBMED    19159624
REFERENCE   2  (bases 1 to 11222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2009-03-15"; volume: "327(2)"; pages: "590-602. Epub 2009 Jan
            3"
COMMENT     Sequence Label: pGFP-IBGFTet
FEATURES             Location/Qualifiers
     source          1..11222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..696
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(693..695,696..698,699..1409)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(693..695,696..698,699..1409)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(738..759)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(999..1018)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1346..1367
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(1476..2050,2051..2053,2054..2062)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1643..1660)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1870..1889
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2075..5116
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             5141..5944
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5204..5223)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5814..5833
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6250..6384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6287..6306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6341..6360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6437..6470
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(6747..7373)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     7278..7297
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        7389..7444
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    7395..7413
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    7425..7443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             7452..8657
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLG
                     FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS
                     LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     protein_bind    8922..8955
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(9034..9054)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9034..9052)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9073..9089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9073..9089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9086..9108)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9097..9113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9121..9127,9128..9145,9146..9151))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9166..9187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9304..9321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9475..10063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9555..9574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10234..11025,11026..11094))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10857..10876
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11095..11199)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccctgctag cttaattaaa ccggtcgcca ccatggtgag caagggcgag gagctgttca
      721 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      781 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      841 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      901 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      961 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1021 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1081 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1141 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1201 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1261 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1321 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1381 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagtc gaggtcgaga
     1441 tctggactct agagaattcc ggcccccccc ccccccccct ctccctcccc cccccctaac
     1501 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     1561 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     1621 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     1681 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     1741 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     1801 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     1861 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     1921 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     1981 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     2041 cgatgataag cttgccacaa ccatggaaga tcccgtcgtt ttacaacgtc gtgactggga
     2101 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2161 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2221 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
     2281 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
     2341 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
     2401 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     2461 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     2521 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
     2581 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct
     2641 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
     2701 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
     2761 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     2821 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     2881 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
     2941 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     3001 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     3061 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     3121 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
     3181 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
     3241 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
     3301 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
     3361 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     3421 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
     3481 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
     3541 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
     3601 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
     3661 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
     3721 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
     3781 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
     3841 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
     3901 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
     3961 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
     4021 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
     4081 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
     4141 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
     4201 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
     4261 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
     4321 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
     4381 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
     4441 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
     4501 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
     4561 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
     4621 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
     4681 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
     4741 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
     4801 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
     4861 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
     4921 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
     4981 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
     5041 ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca
     5101 gttggtctgg tgtcagggga tcccccgggc tgcagccaat atgggatcgg ccattgaaca
     5161 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5221 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5281 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5341 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5401 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5461 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5521 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5581 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5641 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct
     5701 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5761 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5821 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5881 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5941 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6001 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6061 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccccg
     6121 gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta
     6181 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     6241 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6301 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6361 aatgtatctt atcatgtctg gatccggggg taccgcgtcg agcgccagtg tgatggatat
     6421 ctgcagaatt cggcttgaag ttcctattct ctagaaagta taggaacttc agatctatga
     6481 ttccctttgt caacagcaat ggatcactga aaatggttca atgatcacat taagtggtat
     6541 tcaatatttt catgaaatgg gaattgacgt tccttccaaa cattcacgta aaatctgttg
     6601 tgcgtgttta gattggagtg aacgccgttt ccatttaggt gggtacgttg gagccgcatt
     6661 attttcgctt tatgaatcta aagggtggtt aactcgacat cttggttacc gtgaagttac
     6721 catcacggaa aaaggttatg ctgcttttaa gacccacttt cacatttaag ttgtttttct
     6781 aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca
     6841 aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt
     6901 tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc
     6961 acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct
     7021 aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt
     7081 gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa
     7141 tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca
     7201 atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc
     7261 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
     7321 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc
     7381 taatttttgt tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag
     7441 agaaaagtga aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca
     7501 tggggattgg ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg
     7561 aagatatcgc taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct
     7621 ttgctccttg gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt
     7681 cattaatagg cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc
     7741 tgtatttagg ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg
     7801 tcattgccga taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa
     7861 gttttgggct tggtttaata gcggggccta ttattggtgg ttttgcagga gagatttcac
     7921 cgcatagtcc cttttttatc gctgcgttgc taaatattgt cactttcctt gtggttatgt
     7981 tttggttccg tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga
     8041 cgcaatcgaa ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt
     8101 atttttcagc gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa
     8161 atcgttttgg atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac
     8221 actcagtatt ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg
     8281 cagtactgct cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg
     8341 aaggttggtt agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg
     8401 cattacaggg agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat
     8461 tattggtgag ccttaccaat gcaaccggtg ttattggccc attactgttt gctgttattt
     8521 ataatcattc actaccaatt tgggatggct ggatttggat tattggttta gcgttttact
     8581 gtattattat cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac
     8641 aggagacaag tgcttagtta tttcgtcacc aaatgatgtt attccgcgaa atataatgac
     8701 cctcttgata acccaagagg gcatttttta cgataaagaa gatttagctt caaataaaac
     8761 ctatctattt tatttatctt tcaagctcaa taaaaagccg cggtaaatag caataaattg
     8821 gcctttttta tcggcaagct cttttaggtt tttcgcatgt attgcgatat gcataaacca
     8881 gccattgagt aagtttttaa gcacatcatc atcataagct tgaagttcct attctctaga
     8941 aagtatagga acttcaagcc gaattccagc acactggcgg ccgttactag ttctagagcg
     9001 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     9061 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9121 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9181 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9241 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9301 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9661 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9721 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9781 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9841 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9901 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9961 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10201 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10261 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10321 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10381 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10441 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10501 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10561 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10621 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10681 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10741 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10801 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10861 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10921 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10981 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11041 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11101 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11161 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11221 ac
//
