LOCUS       sequence-444661-        1314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of C-terminally TEV cleavable His10-tagged WzxE from E. 
            coli in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Le Bas A, Clarke BR, Teelucksingh T, Lee M, El Omari K, Giltrap AM, 
            McMahon SA, Liu H, Beale JH, Mykhaylyk V, Duman R, Paterson NG, Ward
            PN, Harrison PJ, Weckener M, Pardon E, Steyaert J, Liu H, Quigley A,
            Davis BG, Wagner A, Whitfield C, Naismith JH
  TITLE     Structure of WzxE the lipid III flippase for Enterobacterial Common 
            Antigen polysaccharide.
  JOURNAL   Open Biol. 2025 Jan;15(1):240310. doi: 10.1098/rsob.240310. Epub 
            2025 Jan 8.
  PUBMED    39772807
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2025-01"; volume: "15(1):240310. doi"; pages: " 
            10.1098/rsob.240310. Epub 2025 Jan 8"
COMMENT     Sequence Label: pBAD-WzxE-TEV-His10
FEATURES             Location/Qualifiers
     source          1..1314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1258..1278
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1282..1311
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 atggtgtcgt tggcaaaagc gtccttgtgg acggcggcca gtacactggt caagattggt
       61 gccgggttac tggtcggtaa gttgctggcg gtgtcatttg gtccggcggg gcttgggctg
      121 gcggcaaatt tccgccagtt gattaccgtg ctcggcgtgc ttgccggggc tggcatcttt
      181 aacggtgtaa ccaaatacgt tgcccagtac catgataatc cgcaacagct gcgccgcgtg
      241 gtcggcactt catcagcgat ggtacttggt ttctctacgc tgatggcgct ggtttttgtg
      301 ctggcagctg cgccaatcag ccagggattg tttggtaata ccgactatca ggggctggtg
      361 cgtttagtgg cgctggtgca aatggggatc gcctggggca acctgttact ggcgctgatg
      421 aaaggctttc gcgatgccgc aggtaatgcg ttatcgctga ttgtcggcag cttgattggc
      481 gttctcgcgt actacgtcag ttaccgtttg ggcggttatg aaggggcgtt gctgggtctg
      541 gcgctgattc ccgcgctggt ggtaattcct gccgccatca tgttgatcaa acgtggtgtc
      601 atcccgttaa gctatctgaa acccagctgg gataacggtc tggcagggca gttgagcaaa
      661 tttacgctca tggcgttgat tacgtcggtg accttgcctg ttgcttacat catgatgcgt
      721 aaactgctgg cggcgcagta tagctgggat gaggtgggga tctggcaagg ggtgagcagt
      781 atttccgatg cctacctgca atttattacg gcatcgttca gcgtatattt gctgcccacg
      841 ttgtcgcggc taacggaaaa gcgcgatatc acccgggaag tggttaaatc gctgaaattc
      901 gtcttaccgg cagtggcggc ggcgagtttt accgtctggc tgctgcgtga ttttgctatc
      961 tggctgctgt tgtcgaataa atttaccgct atgcgcgatc tctttgcctg gcagttagtg
     1021 ggtgatgtgt taaaagtggg cgcttatgtc tttggttatc tggtgatcgc caaagcgtca
     1081 ctgcggtttt atattctggc ggaagtcagc cagttcactt tattgatggt atttgcccac
     1141 tggctaatcc ctgcgcatgg tgcactgggc gcggcgcagg catatatggc aacttatatc
     1201 gtctattttt ctctttgttg tggcgtgttt ttactctggc gtaggcgggc actcgaggaa
     1261 aacctgtatt ttcagggcgc gcaccaccac caccaccatc accatcacca ttga
//
