LOCUS       sequence-444683-       14422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Green JJ, Zhou BY, Mitalipova MM, Beard C, Langer R, Jaenisch R, 
            Anderson DG
  TITLE     Nanoparticles for gene transfer to human embryonic stem cell 
            colonies.
  JOURNAL   Nano Lett. 2008 Oct . 8(10):3126-30.
  PUBMED    18754690
REFERENCE   2  (bases 1 to 14422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nano Lett. 
            2008 Oct . 8(10):3126-30."
COMMENT     Sequence Label: human oct4-GFP
FEATURES             Location/Qualifiers
     source          1..14422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2549..3048
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2584..2607)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2981..3000
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3088..3687
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3088..3107)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3584..3604
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4229..4245
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4230..4246
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(8322..8324,8325..8327,8328..9038)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8322..8324,8325..8327,8328..9038)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8367..8388)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8628..8647)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8975..8996
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          9070..9642
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(12055..12074)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12056..12074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12161..12178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12332..12920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12412..12431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13091..13882,13883..13951))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13714..13733
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13952..14056)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14124..14142
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14180..14202)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14302..14321
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        14402..14420
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     14402..14419
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcgaggtgga caccaccctc aagagcctga gccagcagat cgagaacatc cggagcccag
       61 agggcagccg caagaacccc gcccgcacct gccgtgacct caagatgtgc cactctgact
      121 ggaagagtgg tgagggcctg ccctagcctc tccctcccac ctactcctgc catgccaggg
      181 tccccatgcc catatgtgcc cctaccatat ggtgctggct gctccctttc cctgactcca
      241 tcttgccctg ccctaccaca ggagagtact ggattgaccc caaccaaggc tgcaacctgg
      301 atgccatcaa agtcttctgc aacatggaga ctggtgagac ctgcgtgtac cccactcagc
      361 ccagtgtggc ccagaagaac tggtacatca gcaagaaccc caaggacaag aggcatgtct
      421 ggttcggcga gagcatgacc gatggattcc aggtgcgtga gctggacctc agagccagtg
      481 ttaggagatg ggctagccca gtgctcagaa gggacatgaa gtcctggagt aggtctctgc
      541 taagggtgat ggacagagct gggctgggag gcaggggtct caggtccctg atagtggttc
      601 agacacaggc tgccgatggg caggtggtgc ctcctctcga tataacggtg cattgggcag
      661 ctctctgagg accctggaca gggaggccag gcaggactag aggttcccgc atagcggctc
      721 actcttccct ctctcccctc ccctgcagtt cgagtatggc ggccagggct ccgaccctgc
      781 cgatgtggcc atccagctga ccttcctgcg cctgatgtcc accgaggcct cccagaacat
      841 cacctaccac tgcaagaaca gcgtggccta catggaccag cagactggca acctcaagaa
      901 ggccctgctc ctccagggct ccaacgagat cgagatccgc gccgagggca acagccgctt
      961 cacctacagc gtcactgtcg atggctgcac ggtgagtgcc cagaatcccc aggcagggcc
     1021 ccacctctcc ggccttgggc tttttggcca ggccatagtg ccctctctcc atcactccca
     1081 cgtggtaatg ccccctcccg ttgtctccgc cccaccccag agtcacaccg gagcctgggg
     1141 caagacagtg attgaataca aaaccaccaa gacctcccgc ctgcccatca tcgatgtggc
     1201 ccccttggac gttggtgccc cagaccagga attcggcttc gacgttggcc ctgtctgctt
     1261 cctgtaaact ccctccatcc caacctggct ccctcccacc caaccaacct tccccccaac
     1321 ccggaaacag acaagcaacc caaactgaac cccctcaaaa gccaaaaaat gggagacaat
     1381 ttcacatgga ctttggaaaa tatttttttc ctttgcattc atctctcaaa cttagttttt
     1441 atctttgacc aaccgaacat gaccaaaaac caaaagtgca ttcaacctta ccaaaaaaaa
     1501 aaaaaaaaaa agaataaata aataactttt taaaaaagga agcttggtcc acttgcttga
     1561 agacccatgc gggggtaagt ccctttctgc ccgttgggct tatgaaaccc caatgctgcc
     1621 ctttctgctc ctttctccac accccccttg gggcctcccc tccactcctt cccaaatctg
     1681 tctccccaga agacacagga aacaatgtat tgtctgccca gcaatcaaag gcaatgctca
     1741 aacacccaag tggcccccac cctcagcccg ctcctgcccg cccagcaccc ccaggccctg
     1801 ggggacctgg ggttctcaga ctgccaaaga agccttgcca tctggcgctc ccatggctct
     1861 tgcaacatct ccccttcgtt tttgaggggg tcatgccggg ggagccacca gcccctcact
     1921 gggttcggag gagagtcagg aagggccaag cacgacaaag cagaaacatc ggatttgggg
     1981 aacgcgtgtc aatcccttgt gccgcagggc tgggcgggag agactgttct gttccttgtg
     2041 taactgtgtt gctgaaagac tacctcgttc ttgtcttgat gtgtcaccgg ggcaactgcc
     2101 tgggggcggg gatgggggca gggtggaagc ggctccccat tttataccaa aggtgctaca
     2161 tctatgtgat gggtggggtg gggagggaat cactggtgct atagaaattg agatgccccc
     2221 ccaggccagc aaatgttcct ttttgttcaa ggtctatttt tattccttga tatttttctt
     2281 tttttttttt tttgtggatg gggacttgtg aatttttcta aaggtgctat ttaacatggg
     2341 aggagagcgt gtgcggctcc agcccagccc gctgctcact ttccaccctc tctccacctg
     2401 cctctggctt ctcaggcctc tgctctccga cctctctcct ctgaaaccct cctccacagc
     2461 tgcagcccat cctcccggct ccctcctagt ctgtcctgcg tcctctgtcc ccgggtaccg
     2521 ggccccccct cgacctcgaa attctaccgg gtaggggagg cgcttttccc aaggcagtct
     2581 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     2641 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     2701 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     2761 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     2821 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     2881 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     2941 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     3001 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcatccat
     3061 ctagatctcg agcagctgaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     3121 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     3181 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     3241 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     3301 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     3361 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     3421 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     3481 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     3541 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     3601 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     3661 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     3721 aggagcgcac gaccccatgc atcgatgata tcagatcccc gggatgcaga aattgatgat
     3781 ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga
     3841 agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt
     3901 ggggtgggat tagataaatg cctgctcttt actgaaggct ctttactatt gctttatgat
     3961 aatgtttcat agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca
     4021 ttcctcccac tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt
     4081 gattcccact ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga
     4141 agaacgagat cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag
     4201 ttctaattcc atcagaagct ggtcgaggtc gaggtcgacg gtatcgataa gcttggtacc
     4261 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgcccttc agggtacagg
     4321 gaggcctatg ccaacttctc attgaacacc actcccagct ttcctacccc catttctctt
     4381 cacaggtgtg cagagaagtc tacattccca tgtcaagtaa ggggggtggg tagatgggag
     4441 ggcatatgag agcggggcag caattttatg tcttagttgg aaggaagttt agagttcatt
     4501 gagtccaaat cctctttact aggtgaggaa actgaggcac aggaggggga ggtgccatta
     4561 gccagggagt cagagccagg acttagaacc tggtccatgc ctcctagttt gttcctgacc
     4621 aatcctccca gctcacccac tccttttgct ctcacctctg actccctaca ggctacagct
     4681 agagagttga ctcctctatt tgaggtaaga gtgtctggat ttgggataag gtgacctcag
     4741 tttgcaggtc tcatggggga gatcagaggg tcctttgttg agaagagggg acttggtcca
     4801 cagtgagcaa aggaatttgt ctaaattgct attaagggag atcatctccc aaggtgggcc
     4861 aagcctgaac cctcagccct ccatttcttc cctttctttt gccttcagat tgacaggcct
     4921 cggaagtcaa aataagtggt ttcctagacc gggtcgagag caagtctcta ttggtcccaa
     4981 ctgagttttt tcagctggtt tttcaaccaa acagcacctc atctcccagt gaggggaagg
     5041 gaaggctggg ctgagagcag caaggctgct catctcacct ctccccaccc agccatgcca
     5101 gccgccacac ctggtgggga gaggtgggcc tcacctgggt cccctggcag tgctctgtga
     5161 agggtcttga cattgcactg taataataaa ggtgtgtgtg aagtatcttt ttatggtgac
     5221 tttctaaaac ccagggaatc atgggaccag ttctgatgac tcagcctggc ttccagtctc
     5281 ctccaggccc aacggtggcc cccagcactg gttggggcct gggagagctg gccttgactg
     5341 aagtgaagcc acctaccctt caggcataac aggacagtga gaaggaagga aagcctgcct
     5401 caacctccca tcagccctga gcaccccaga agggggccgg ctaggagtct aggcatgcag
     5461 gaggctgacc cctgactggg ctcatatcca gccacaaggc agccagggac ccaggcaccc
     5521 accccttgtc tgcgtccctc tcgggaatgg gcctcttgcc caggccagaa atacaccacc
     5581 tacagtacaa attataatct aaaaacaaga gggtggtgtt gagtggggaa attggggaag
     5641 gtgttttagg agccactagg aaaatgggca gcagggactc tctggactgg cttgggagga
     5701 gcgcttttgg ggaacctgga ggatggcaag ctgagaaaca ctggtgtgga gattccagcc
     5761 aaatcccagg cctgcccctc cccctcctct gagaggccgt cttcttggca gacagcagag
     5821 agatgcatga caaaggtgcc gtgatggttc tgtcctgggg attgagatgg ctggggaggg
     5881 gcctcctcct gttccgaagc atgttcctcc tacccccacc aggccccata atctacctgc
     5941 cttttgggca gttaaaggcc gagaagtgaa cacagctgca accccactgc cttgtagacc
     6001 ttccggtaga cctgtggcag gtattgaaat gcacgcatac aattaggctc aaaaagtcta
     6061 cacagacagg agatgggcac acgaacagag gcaacataag agtgggggaa aagtctcaaa
     6121 agactcacgg atgccaccaa gatgaagaca gctggccacg ggacacccat ccccttagaa
     6181 ggcagataga gccactgacc tcagcagaca agcccaggca gggctgagcc tggagcctgc
     6241 aatgagaagc cttacttaag tcgacagagg tcagcgtgcc cagtccagac ctggccttct
     6301 ggccttcgaa gctgtgggga gccctggccc agagccccct ctggagcccc cagacttacc
     6361 ccaggccctc cactgagatc aagttttggg agcagacaga caaacatcat ccctcacaga
     6421 caggcattcc gttggctatt ctcttgcaaa cagaatcaag cactagacca gcagcatgag
     6481 cctcaggata ctcaggccag gcccagaaaa acagaccctg aaggggagct tagggcagcc
     6541 ttcctgcacg cctccacaaa tcactctcca cctcctctgc gtctttctgc cagccagccc
     6601 cactaaacaa agcacatccc tcaatctgcc aggctcgggg agggacgcac gatgaagctg
     6661 gacgcctgag tcccccagag gaaggaggaa ctagatacct aggtccctgt ggggggccct
     6721 tggtgcccgt ctgaggctca gtctttgagg ggattgcaga ggggggttgc tggagctcct
     6781 tttagcgtct ctgaagggga ttctgtgtga ggggattggg actggggggt tggggagcag
     6841 gaagcagtcc ccaggggagc catccaggcc cattcaaggg ttgagcactt gtttagggtt
     6901 agagctgccc cctctgggga ccgggattgt ccagccaagg ccattgtcct gcccccttcc
     6961 cccagtccct cccaggcttc tttgaacctg aagtcagata ttttttctcc acacccccca
     7021 ccccctggtt ttccccaccc agggcctagg gctggaggcc tgggccaggg aggtggggga
     7081 gggagaacgg ggcctaccgt ggtattagat gtctgagttt tggttgagag gggagcaagg
     7141 aacctgatgt gcaggttcca tagtggaggg ggcccaaagc gggtgtctta tcactctgtt
     7201 tcagcaaagg ttgggaaact gaggcccagt cagtccaaag tctggtccct tgaaggggaa
     7261 gtagggacca accccttagt ctgttagatg aggagagcct ggagtctgat tctggaagac
     7321 ggaggggtgg ggggatgggg gggtgggggg atatagcacg gaggccttgt ctggcagtct
     7381 actcttgaag atggggtgaa atttggcagg ctgggcagat ggtgccaggc acccaggctg
     7441 cggggtggct ggatttggcc agtatcggga tgggaatgcc taggattctg gatggatcgg
     7501 gggaaggcat aagggagcag ctggccattg tgcttatggc tgttgatgca ttgagggata
     7561 gcgccacaca cacattcaat aaatttgagg agctgagagg gtgactggcc cctgaaggca
     7621 cagtgccaga ggtctgtgga gagggggtca agcacctggg ttcctgaaga acatggagtt
     7681 gtgggagtga ttccagacag ctgggatgtg cagagcctga gagagtgcca gggagcgggt
     7741 tgggagttga aagttgggtg tggtggctca cgcctttaat catgacactg ggcggcagag
     7801 gtgggaggat ttcttgagga caggaattca agaccagcct gggtaacata gcaaggcccc
     7861 atctctacta aaaataaaaa aactaacagg gcacagtggt ccaagcctgt agtcccagcc
     7921 aattaggagg ctggagcaga aggattgctt tggcccagta gatcgaggct acattgagcc
     7981 atcattgtac tccactgcac tccagtctgg gcaacaaagt gagaccctgt cttaaaaaat
     8041 aaaaataaaa aaagtttctg tgggggacct gcactgaggt cctggagggg cgccagttgt
     8101 gtctcccggt tttccccttc cacagacacc attgccacca ccattaggca aacatccttc
     8161 gcctcagttt ctccccccac ctccctctcc tccacccatc cagggggcgg ggccagaggt
     8221 caaggctagt gggtgggact ggggagggag agaggggttg agtagtccct tcgcaagccc
     8281 tcatttcacc aggcccccgg cttggggcgc cttccttccc catggtgagc aagggcgagg
     8341 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     8401 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     8461 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     8521 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     8581 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     8641 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     8701 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     8761 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     8821 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     8881 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     8941 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     9001 ccgccgggat cactctcggc atggacgagc tgtacaagac tagttattga ggatcctgag
     9061 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
     9121 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
     9181 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
     9241 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
     9301 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
     9361 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
     9421 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
     9481 ggaaatattc ttattggtag aaacaactac accctggtca tcatcctgcc tttctcttta
     9541 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     9601 ccctctgcta accatgttca tgccttcttc tctttcctac agctcctggg caacgtgctg
     9661 gttgttgtgc tgtctcatca ttttggcaaa ggattcactc ctcaggtgca ggctgcctat
     9721 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcgtttt
     9781 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     9841 taaaggaaat ttattttcat tgcaatagtg tgttggaatt tctcgatcgc tagagcggcc
     9901 gccaccgcgg tggagctcag agacaacttc ccaaagcaca aagcagtttt tccccctagg
     9961 ggtgggagga agcaaaagac tctgtaccta ttttgtatgt gtataataat ttgagatgtt
    10021 tttaattatt ttgattgctg gaataaagca tgtggaaatg acccaaacat aatccgcagt
    10081 ggccccctaa tttccttctt tggagttggg ggaggggtag acatggggaa ggggctttgg
    10141 ggtgatgggc ttgccttcca ttcctgccct ttccctcccc actattctct tctagatccc
    10201 tccataaccc cactcccctt tctctcaccc ttcttatacc gcaaaccttt ctacttcctc
    10261 tttcattttc tattcttgca atttccttgc accttttcca aatcctcttc tcccctgcaa
    10321 taccatacag gcaatccacg tgcacaacac acacacacac tcttcacatc tggggttgtc
    10381 caaacctcat acccactccc cttcaagccc atccactctc caccccctgg atgccctgca
    10441 cttggtggcg gtgggatgct catggatact gggagggtga ggggagtgga acccgtgagg
    10501 aggacctggg ggcctctcct cgaactgaca tgaagggtca tctggcctct gctcccttct
    10561 cacccacgct gacctcctgc cgaaggagca acgcaacagg agaggggtct gctgagcctg
    10621 gcgagggtct gggagggacc aggaggaagg cgtgctccct gctcgctgtc ctggccctgg
    10681 gggagtgagg gagacagaca cctgggagag ctgtggggaa ggcactcgca ccgtgctctt
    10741 gggaaggaag gagacctggc cctgctcacc acggactggg tgcctcgacc tcctgaatcc
    10801 ccagaacaca acccccctgg gctggggtgg tctggggaac catcgtgtcc ccgcctcccg
    10861 cctactcctt tttaagcttt ttctttcttt ctttcttttt tttcttaaat tcttgcttcc
    10921 tcttcccccc tcttctccgt cccttctctt tcccttcacc ttctgcctgc tccttcccag
    10981 aggccctgag aaggcgaggc ccggagggaa ggtgactgtt cggctccccc atctcaggta
    11041 agggggacag cgagtgtggt cagcggggga cagagaatcc agggcgtcag aagtccagag
    11101 agaaccaggc acccggagcc agggagacag cgagacgaca gctggtcacc tctctcacct
    11161 catctcaggt ctaaacccaa aaagaacaaa aagcccgcac tcctccctaa ggtctccaga
    11221 gagatggaca agtctgtaga taccatccat cagcaatact aatatcgtga tgtcattaag
    11281 tattcatggc cgttcattat ggccttcaat aattcatcat tagcacttct accacactta
    11341 ggacagtgta gaaagggaat cagtgtagag aggataagaa tgatggcata taaatgaagg
    11401 cttattcata tgcaaattac ccaaagttgt gtgatgccta tccatctctc tttctctctt
    11461 ttttttgaga cagagtctca ctctgtcacc caggctggag tgcggtggcg cagtctcagc
    11521 tcactgcaac ctccgcctcc caggttcaag caattctcat gcctcagcct cccaagtaac
    11581 tgggatgaca gccatgcact accacgccca gctaattttt atatttttag tagagtcagg
    11641 gttttaccat gttagccagc ctggtctcga actcctggcc tcaagtgatc cacctgcctc
    11701 agcctctcaa agtgctggga ttgcggacat gagccacagc accaggctcc atctcttgat
    11761 caatgtgttg attgcttggt gatgcctgcc atgctgccca ggaaaccctg tccacttgga
    11821 cctctgggac tttaggaccc tggctgtcca ggccacatcc ccatctgccc tcgtggggga
    11881 cccagggaca tcctgagcct ggcatccaag gctctacctg gccactaccc acctgcccag
    11941 gctcactgtc ccctcccctg cccacatctg tcacaccaac caaaaagggt ggccaacagt
    12001 tctcacaaat gcactgcatt tccccacttc caggccttct agatctgccg gtctccctat
    12061 agtgagtcgt attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg
    12121 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    12181 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    12241 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    12301 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    12361 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    12421 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    12481 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    12541 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    12601 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    12661 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    12721 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    12781 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    12841 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    12901 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    12961 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    13021 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    13081 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    13141 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    13201 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    13261 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    13321 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    13381 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    13441 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    13501 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    13561 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    13621 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    13681 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    13741 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    13801 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    13861 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    13921 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    13981 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    14041 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    14101 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    14161 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    14221 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    14281 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgg
    14341 acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
    14401 gatttaggtg acactataga ac
//
