LOCUS       sequence-444714-        1683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All threonines in the LC domain to valine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 TtoV
FEATURES             Location/Qualifiers
     source          1..1683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             963..980
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1033..1051)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1047..1094
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1131..1586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1218..1237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1428..1449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc tctgataaaa
       61 ttcatcacca tcaccatcac gaaaatcttt attttcaagg tatggcgagc aacgattacg
      121 ttcagcaagc ggtgcagagc tatggtgcgt acccggttca accgggtcag ggttacagcc
      181 aacagagcag ccaaccgtat ggtcaacaga gctatagcgg ttacagccaa agcgtggacg
      241 ttagcggtta tggtcaaagc agctacagca gctatggtca gagccaaaac gttggctacg
      301 gtgttcagag cgttccgcag ggctacggta gcgtgggtgg ttatggtagc agccaaagca
      361 gccagagcag ctacggtcag caaagcagct acccgggtta tggccagcaa ccggcgccga
      421 gcagcgttag cggtagctac ggtagcagca gccagagcag cagctatggt caaccgcaaa
      481 gcggcagcta tagccaacag ccgagctatg gcggtcagca acagagctac ggtcaacagc
      541 aaagctataa cccgccgcag ggttatggcc aacagaacca gtacaacagc agcagcggtg
      601 gcggtggcgg tggtggcggc ggtggtaact acggtcagga ccaaagcagc atgagcagcg
      661 gcggtggtag cggtggtggt tacggtaacc aggatcaaag cggcggtggc ggtagcggtg
      721 gctacggtca acaggatcgt ggtggccgtg gtcgtggtgg cagcggcggc ggtggtggtg
      781 gtggcggcgg tggctacaac cgtagcagcg gtggttatga accgcgtggt cgtggcggtg
      841 gtcgtggtgg tcgtggcggt atgggcggta gcgaccgtgg cggttttaac aagttcggcg
      901 gtccgcgtga tcagggtagc cgtcacgaca gcgagcagga caacagcgac aactgactcg
      961 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     1021 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     1081 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
     1141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1561 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     1621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta
     1681 gaa
//
