LOCUS       sequence-444716-        1683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All threonines in the LC domain to serine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 TtoS
FEATURES             Location/Qualifiers
     source          1..1683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             963..980
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1033..1051)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1047..1094
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1131..1586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1218..1237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1428..1449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc tctgataaaa
       61 ttcatcacca tcaccatcac gaaaatcttt attttcaagg tatggcgagc aacgattata
      121 gccagcaagc gagccagagc tacggtgcgt atccgagcca accgggccag ggttacagcc
      181 agcaaagcag ccagccgtat ggtcagcaga gctacagcgg ctatagccag agcagcgaca
      241 gcagcggtta tggccagagc agctacagca gctatggtca aagccagaac agcggttacg
      301 gtagccaaag cagcccgcag ggctatggta gcagcggtgg ctacggtagc agccagagca
      361 gccaaagcag ctacggccag caaagcagct acccgggtta tggtcagcag ccggcgccga
      421 gcagcagcag cggtagctat ggcagcagca gccaaagcag cagctacggt cagccgcaaa
      481 gcggtagcta cagccagcaa ccgagctacg gtggtcaaca gcaaagctac ggtcaacagc
      541 agagctataa cccgccgcaa ggttatggcc aacagaacca gtacaacagc agcagcggcg
      601 gcggtggtgg tggcggtggc ggcggtaact acggtcaaga ccagagcagc atgagcagcg
      661 gtggtggcag cggtggcggc tatggtaacc aggatcaaag cggtggcggt ggtagcggtg
      721 gctatggtca gcaagaccgt ggtggccgtg gccgtggtgg tagcggcggt ggcggcggtg
      781 gcggtggtgg cggttacaac cgtagcagcg gcggttatga accgcgtggt cgtggcggtg
      841 gtcgtggtgg ccgtggtggc atgggcggta gcgatcgtgg tggcttcaac aaatttggtg
      901 gcccgcgtga tcaaggcagc cgtcacgaca gcgaacagga taacagcgac aactgactcg
      961 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     1021 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     1081 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
     1141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1561 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     1621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta
     1681 gaa
//
