LOCUS       sequence-444728-       16786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mTALEAD expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16786)
  AUTHORS   Zhou C, Okuno M, Nakazato I, Tsutsumi N, Arimura SI
  TITLE     Targeted A-to-G base editing in the organellar genomes of 
            Arabidopsis with monomeric programmable deaminases.
  JOURNAL   Plant Physiol. 2023 Dec 21:kiad678. doi: 10.1093/plphys/kiad678.
  PUBMED    38128544
REFERENCE   2  (bases 1 to 16786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 21:kiad678. doi: 10.1093/plphys/kiad678."
COMMENT     Sequence Label: mTALEAD nad7-2
FEATURES             Location/Qualifiers
     source          1..16786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2246..2270)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2283..2285,2286..2288,2289..2996)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(2283..2285,2286..2288,2289..2996)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(2328..2349)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2589..2608)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2936..2957
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3174..3183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        3522..3701
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3735..4529
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3789..3808)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4399..4418
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4569..4821
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4914..4938
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5459..6250
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6496..7084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6985..7004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7238..7255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7270..7410
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7336..7355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7496..7518
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7754..7948
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8014..9087)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9516..10145)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11476..11500
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    13342..13366
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             13369..13395
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(16510..16534)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggccctctag aggatccccg gcaccctact tagatcaaca cataaaagtt agtaagtgaa
       61 gaaccacaac aacaacacta gattcatctt caagtgtatg taggtatagt aacatgaaca
      121 agaacagact caagtacaag atcgcatacg aaaatggaaa tggcaatgtc acttccacat
      181 aatcaaacac gaatcctcat atcaacaagg cctgagattc taactagctc ataacaactt
      241 agccaatagt tacttgagac taccaaatgt atgtagaact aaagactaag ggacagagag
      301 ttcgtctaaa caggtgaatc tagtcgttgt tatctaataa acaattcagc cccaaatgca
      361 gaacacacat agagctctct attgattcaa attacgatct gatactgata acgtctagat
      421 ttttagggtt aaagcaatca atcacctgac gattcaaggt ggttggatca tgacgattcc
      481 agaaaacatc aagcaagctc tcaaagctac actctttggg atcatactga actctaacaa
      541 cctcgttatg tcccgtagtg ccagtacaga catcctcgta actcggattg tgcacgatgc
      601 catggctata cccaacctcg gtcttggtca caccaggaac tctctggtaa gctagctcca
      661 ctccccagaa acaaccggcg ccaaattgcg cgaattgctg acctgaagac ggaacatcat
      721 cgtcgggtcc ttgggcgatt gcggcggaag atgggtcagc ttgggcttga ggacgagacc
      781 cgaatccgag tctgttgaaa aggttgttca ttggggattt gtatacggag attggtcgtc
      841 gagaggtttg agggaaagga caaatgggtt tggctctgga gaaagagagt gcggctttag
      901 agagagaatt gagaggttta gagagagatg cggcggcgat gagcggagga gagacgacga
      961 ggacctgcat tatcaaagca gtgacgtggt gaaatttgga acttttaaga ggcagataga
     1021 tttattattt gtatccattt tcttcattgt tctagaatgt cgcggaacaa attttaaaac
     1081 taaatcctaa atttttctaa ttttgttgcc aatagtggat atgtgggccg tatagaagga
     1141 atctattgaa ggcccaaacc catactgacg agcccaaagg ttcgttttgc gttttatgtt
     1201 tcggttcgat gccaacgcca cattctgagc taggcaaaaa acaaacgtgt ctttgaatag
     1261 actcctctcg ttaacacatg cagcggctgc atggtgacgc cattaacacg tggcctacaa
     1321 ttgcatgatg tctccattga cacgtgactt ctcgtctcct ttcttaatat atctaacaaa
     1381 cactcctacc tcttccaaaa tatatacaca tctttttgat caatctctca ttcaaaatct
     1441 cattctctct agtaaacaag aacaaaaaaa tggcggatac agctagagga acccatcacg
     1501 atatcatcgg cagagaccag tacccgatga tgggccgaga ccgagaccag taccagatgt
     1561 ccggacgagg atctgactac tccaagtcta ggcagattgc taaagctgca actgctgtca
     1621 cagctggtgg ttccctcctt gttctctcca gccttaccct tgttggaact gtcatagctt
     1681 tgactgttgc aacacctctg ctcgttatct tcagcccaat ccttgtcccg gctctcatca
     1741 cagttgcact cctcatcacc ggttttcttt cctctggagg gtttggcatt gccgctataa
     1801 ccgttttctc ttggatttac aagtaagcac acatttatca tcttacttca taattttgtg
     1861 caatatgtgc atgcatgtgt tgagccagta gctttggatc aatttttttg gtcgaataac
     1921 aaatgtaaca ataagaaatt gcaaattcta gggaacattt ggttaactaa atacgaaatt
     1981 tgacctagct agcttgaatg tgtctgtgta tatcatctat ataggtaaaa tgcttggtat
     2041 gatacctatt gattgtgaat aggtacgcaa cgggagagca cccacaggga tcagacaagt
     2101 tggacagtgc aaggatgaag ttgggaagca aagctcagga tctgaaagac agagctcagt
     2161 actacggaca gcaacatact ggtggggaac atgaccgtga ccgtactcgt ggtggccagc
     2221 acactactca agggtgggcg cgccgaccca gctttcttgt acaaagtggt gatatcccgc
     2281 ggatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2341 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2401 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2461 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2521 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2581 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2641 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2701 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2761 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2821 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2881 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2941 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacgaac
     3001 ccggcatgtt acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
     3061 gggcattcag tctggatcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
     3121 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa tgaccatggc
     3181 ggccgggagg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     3241 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     3301 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     3361 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc
     3421 atgcgacctc gaattatcga tcatgagcgg agaattaagg gagtcacgtt atgacccccg
     3481 ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga
     3541 gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta
     3601 ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat
     3661 gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc
     3721 aactcgatcg aggcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3781 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3841 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3901 ccctgaatga actccaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3961 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4021 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4081 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4141 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4201 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4261 cgcggatgcc cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc ttgccgaata
     4321 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4381 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4441 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4501 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcggac tctagctaga
     4561 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     4621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     4681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     4741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     4801 tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt
     4861 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     4921 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     4981 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     5041 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     5101 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     5161 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     5221 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     5281 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     5341 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     5401 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     5461 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     5521 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     5581 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     5641 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     5701 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     5761 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     5821 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     5881 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     5941 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     6001 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     6061 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     6121 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     6181 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     6241 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     6301 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     6361 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     6421 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6481 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6541 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6601 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     6661 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6721 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6781 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6841 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6901 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6961 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7021 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7081 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7141 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7201 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7261 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7321 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     7381 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     7441 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7501 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     7561 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     7621 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     7681 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     7741 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     7801 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     7861 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     7921 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     7981 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     8041 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     8101 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     8161 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     8221 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     8281 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     8341 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     8401 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     8461 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     8521 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     8581 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     8641 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     8701 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     8761 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     8821 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     8881 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     8941 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     9001 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     9061 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     9121 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     9181 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     9241 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     9301 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     9361 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
     9421 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
     9481 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
     9541 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
     9601 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
     9661 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
     9721 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
     9781 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
     9841 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
     9901 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
     9961 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    10021 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    10081 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    10141 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    10201 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    10261 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    10321 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    10381 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    10441 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    10501 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    10561 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    10621 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    10681 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    10741 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    10801 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    10861 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    10921 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    10981 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    11041 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    11101 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    11161 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    11221 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    11281 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac
    11341 tttaaagtac tttaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
    11401 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
    11461 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
    11521 aaggcgggaa acgacaatct gatccaagct caagctaagc tctcaacttt tgattcgcta
    11581 tttgcagtgc acctgtggcg ttcatcacat cttttgtgac actgtttgca ctggtcattg
    11641 ctattacaaa ggaccttcct gatgttgaag gagatcgaaa gtaagtaact gcacgcataa
    11701 ccattttctt tccgctcttt ggctcaatcc atttgacagt caaagacaat gtttaaccag
    11761 ctccgtttga tatattgtct ttatgtgttt gttcaagcat gtttagttaa tcatgccttt
    11821 gattgatctt gaataggttc caaatatcaa ccctggcaac aaaacttgga gtgagaaaca
    11881 ttgcattcct cggttctgga cttctgctag taaattatgt ttcagccata tcactagctt
    11941 tctacatgcc tcaggtgaat tcatctattt ccgtcttaac tatttcggtt aattaaagca
    12001 cgaacaccat tactgcatgt agaagcttga taaactatcg ccaccaattt atttttgttg
    12061 cgatattgtt actttcctca gtatgcagct ttgaaaagac caaccctctt atcctttaac
    12121 aatgaacagg tttttagagg tagcttgatg attcctgcac atgtgatctt ggcttcaggc
    12181 ttaattttcc aggtaaagca ttatgagata ctcttatatc tcttacatac ttttgagata
    12241 atgcacaaga acttcataac tatatgcttt agtttctgca tttgacactg ccaaattcat
    12301 taatctctaa tatctttgtt gttgatcttt ggtagacatg ggtactagaa aaagcaaact
    12361 acaccaaggt aaaatacttt tgtacaaaca taaactcgtt atcacggaac atcaatggag
    12421 tgtatatcta acggagtgta gaaacatttg attattgcag gaagctatct caggatatta
    12481 tcggtttata tggaatctct tctacgcaga gtatctgtta ttccccttcc tctagctttc
    12541 aatttcatgg tgaggatatg cagttttctt tgtatatcat tcttcttctt ctttgtagct
    12601 tggagtcaaa atcggttcct tcatgtacat acatcaagga tatgtccttc tgaattttta
    12661 tatcttgcaa taaaaatgct tgtaccaatt gaaacaccag ctttttgagt tctatgatca
    12721 ctgacttggt tctaaccaaa aaaaaaaaaa tgtttaattt acatatctaa aagtaggttt
    12781 agggaaacct aaacagtaaa atatttgtat attattcgaa tttcactcat cataaaaact
    12841 taaattgcac cataaaattt tgttttacta ttaatgatgt aatttgtgta acttaagata
    12901 aaaataatat tccgtaagtt aaccggctaa aaccacgtat aaaccaggga acctgttaaa
    12961 ccggttcttt actggataaa gaaatgaaag cccatgtaga cagctccatt agagcccaaa
    13021 ccctaaattt ctcatctata taaaaggagt gacattaggg tttttgttcg tcctcttaaa
    13081 gcttctcgtt ttctctgccg tctctctcat tcgcgcgacg caaacgatct tcaggtgatc
    13141 ttctttctcc aaatcctctc tcataactct gatttcgtac ttgtgtattt gagctcacgc
    13201 tctgtttctc tcaccacagc catgtttaaa caagcttctc gtctcctctc ccgatctgtc
    13261 gccgccgcat cttccaaatc ggtgacgact cgtgcctttt caacggaact tccatcgacg
    13321 ctcgattccg ctagtgatat cacaagtttg tacaaaaaag caggctttta cccatacgat
    13381 gttccagatt acgctgatat cgccgatcta cgcacgctcg gctacagcca gcagcaacag
    13441 gagaagatca aaccgaaggt tcgttcgaca gtggcgcagc accacgaggc actggtcggc
    13501 cacgggttta cacacgcgca catcgttgcg ttaagccaac acccggcagc gttagggacc
    13561 gtcgctgtca agtatcagga catgatcgca gcgttgccag aggcgacaca cgaagcgatc
    13621 gttggcgtcg gcaaacagtg gtccggcgca cgcgctctgg aggccttgct cacggtggcg
    13681 ggagagttga gaggtccacc gttacagttg gacacaggcc aacttctcaa gattgcaaaa
    13741 cgtggcggcg tgaccgcagt ggaggcagtg catgcatggc gcaatgcact gacgggtgcc
    13801 ccgctcaacc tgaccccgga ccaggtggtt gcaatcgcgt cacacgatgg gggaaagcag
    13861 gccctagaaa ccgttcagcg actcctgccc gtcctgtgcc aggaccacgg cctgaccccc
    13921 gaacaggttg tcgctattgc tagtaacggc ggaggcaaac aggcgctgga aacagttcag
    13981 cgcctcttgc cggtcttgtg tcaggcccac ggcctgaccc cagaccaagt tgtcgcgatt
    14041 gcaagcaaca acggaggcaa acaagcctta gaaacagtcc agagattgtt gcctgtgctg
    14101 tgccaagccc acggcctgac cccagcccag gttgtggcca tcgccagcaa cataggtggc
    14161 aagcaggccc tcgaaaccgt ccagagactg ttaccggttc tctgccagga ccacggcctg
    14221 accccagacc aggttgtggc catcgccagc aacataggtg gcaagcaggc cctcgaaacc
    14281 gtccagagac tgttaccggt tctctgccag gaccacggcc tgaccccgga acaggtggtt
    14341 gcaatcgcgt cacacgatgg gggaaagcag gccctagaaa ccgttcagcg actcctgccc
    14401 gtcctgtgcc aggcccacgg cctgacccca gaccaagttg tcgcgattgc aagcaacaac
    14461 ggaggcaaac aagccttaga aacagtccag agattgttgc ctgtgctgtg ccaagcccac
    14521 ggcctgaccc cagcccaggt tgtggccatc gccagcaaca taggtggcaa gcaggccctc
    14581 gaaaccgtcc agagactgtt accggttctc tgccaggacc acggcctgac ccccgaccag
    14641 gttgtcgcta ttgctagtaa cggcggaggc aaacaggcgc tggaaacagt tcagcgcctc
    14701 ttgccggtct tgtgtcagga ccacggcctg accccggaac aggtggttgc aatcgcgtca
    14761 cacgatgggg gaaagcaggc cctagaaacc gttcagcgac tcctgcccgt cctgtgccag
    14821 gcccacggcc tgaccccaga ccaggttgtg gccatcgcca gcaacatagg tggcaagcag
    14881 gccctcgaaa ccgtccagag actgttaccg gttctctgcc aggcccacgg cctgacccca
    14941 gcccaagttg tcgcgattgc aagcaacaac ggaggcaaac aagccttaga aacagtccag
    15001 agattgttgc cggtgctgtg ccaagaccac ggcctgaccc cagaccaggt tgtggccatc
    15061 gccagcaaca taggtggcaa gcaggccctc gaaaccgtcc agagactgtt accggttctc
    15121 tgccaggacc acggcctgac cccagaacag gttgtggcca tcgccagcaa cataggtggc
    15181 aagcaggccc tcgaaaccgt ccagagactg ttaccggttc tctgccaggc ccacggcctg
    15241 acccccgacc aggttgtcgc tattgctagt aacggcggag gcaaacaggc gctggaaaca
    15301 gttcagcgcc tcttgccggt cttgtgtcag gcccacggcc tgacccctga gcaggtagtg
    15361 gctattgcat ccaacatagg gggcagaccc gcactggagt caattgttgc ccagttatct
    15421 cgccctgatc cggcgttggc cgcgttgacc aacgaccacc tcgtcgcctt ggcctgcctc
    15481 ggcgggcgtc ctgcgctgga tgcagtgaaa aagggattgg gggggtctgg atcgtatgct
    15541 ttaggaccgt atcagatctc aagtgaagtc gagttttcgc atgagtactg gatgagacat
    15601 gcgttgacgt tagccaaacg tgcaagagat gagagagaag tacctgttgg agctgtgttg
    15661 gtcctcaaca atcgcgtaat tggagaaggc tggaatagag ccataggtct tcacgatcca
    15721 acagcccatg cagagatcat ggctctcaga caaggaggtc ttgttatgca gaattaccgt
    15781 ctgatagatg caacgttgta tgtgaccttt gagccttgtg ttatgtgtgc aggcgcgatg
    15841 atccactctc ggattggacg tgtagtattt ggagtgcgaa acagcaaaag gggtgctgct
    15901 ggttctctga tgaatgtcct taactatccg ggtatgaacc atagggtgga aatcacagag
    15961 ggcattctag ctgacgaatg tgctgctttg ctttgcgact tctaccgaat gcctagacag
    16021 gtgttcaatg cacagaagaa agcgcaaagc tcgatcaacc tcgttggatc agggtcttat
    16081 gctcttggac cctatcagat ttcagcgcct caattaccgg cttataatgg gcaaacagtt
    16141 ggaaccttct actatgtcaa cgatgctggt ggtcttgaga gtaaggtgtt ttcatcagga
    16201 ggtccaactc catacccaaa ctacgctaat gcagggcatg ttgcaggaca atctgctttg
    16261 ttcatgaggg ataatgggat aagtgaaggg ctagtcttcc acaacaatcc ggaaggaact
    16321 tgcggttttt gcgtgaacat gactgaaact ctcttacctg agaatgccaa gatgactgtt
    16381 gttcctccag aaggcgctat tcccgttaaa cggggagcaa ctggtgagac aaaggttttt
    16441 accggcaact ccaattcccc aaaatctcct acaaagggag gttgttgata actcgagcga
    16501 tcctctagaa cccagctttc ttgtacaaag tggtgatatc ccgcggccat gctagagtcc
    16561 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
    16621 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
    16681 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
    16741 taattcctaa aaccaaaatc cagtgacctg caggcatgcg acgtcg
//
