LOCUS       sequence-444730-        1683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All serines in the LC domain to glycine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC-RGG1 StoG
FEATURES             Location/Qualifiers
     source          1..1683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             963..980
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1033..1051)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1047..1094
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1131..1586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1218..1237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1428..1449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc tctgataaaa
       61 ttcatcacca tcaccatcac gaaaatcttt attttcaagg tatggcgggt aacgactaca
      121 cccagcaagc gacccaaggt tatggtgcgt atccgaccca gccgggtcaa ggttatggtc
      181 agcaaggtgg ccagccgtat ggtcagcaag gctacggtgg ctatggtcag ggcaccgata
      241 ccggtggcta cggtcagggt ggctacggtg gctatggcca gggccaaaac accggttatg
      301 gcacccaagg taccccgcaa ggttatggtg gcaccggtgg ctatggtggc ggtcagggcg
      361 gtcaaggcgg ttacggtcag caaggcggtt acccgggtta tggtcaacaa ccggcgccgg
      421 gcggtaccgg cggtggctac ggtggcggtg gccagggtgg cggttatggt cagccgcaag
      481 gcggtggcta cggtcagcaa ccgggctatg gtggccagca acagggttac ggccaacagc
      541 aaggttataa cccgccgcag ggttacggcc agcaaaacca atataacggt agcagcggtg
      601 gcggtggcgg tggcggtggc ggtggcaact acggtcagga ccaaagcagc atgagcagcg
      661 gtggcggtag cggcggtggc tacggtaacc aagaccaaag cggtggcggt ggcagcggtg
      721 gctatggtca gcaagatcgt ggtggccgtg gtcgtggtgg cagcggtggc ggtggcggtg
      781 gcggtggcgg tggctacaac cgtagcagcg gtggctatga accgcgtggt cgtggtggcg
      841 gtcgtggcgg tcgtggcggt atgggcggta gcgatcgtgg cggtttcaac aagtttggcg
      901 gtccgcgtga ccagggtagc cgtcacgata gcgaacaaga caacagcgat aactgactcg
      961 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     1021 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     1081 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
     1141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1561 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     1621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta
     1681 gaa
//
