LOCUS       sequence-444731-        1320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All threonines to serine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS phase separation.
  JOURNAL   Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 
            10.1038/s41589-024-01828-6. Epub 2025 Feb 7.
  PUBMED    39920231
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01828-6"; journalName: "Nat Chem Biol"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1076-1088"
COMMENT     Sequence Label: FUS LC TtoS
FEATURES             Location/Qualifiers
     source          1..1320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             600..617
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(670..688)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      684..731
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      768..1223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(855..874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1065..1086
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc tctgataaaa
       61 ttcatcacca tcaccatcac gaaaatcttt attttcaagg tatggcgagc aacgattata
      121 gccagcaagc gagccagagc tacggtgcgt atccgagcca accgggccag ggttacagcc
      181 agcaaagcag ccagccgtat ggtcagcaga gctacagcgg ctatagccag agcagcgaca
      241 gcagcggtta tggccagagc agctacagca gctatggtca aagccagaac agcggttacg
      301 gtagccaaag cagcccgcag ggctatggta gcagcggtgg ctacggtagc agccagagca
      361 gccaaagcag ctacggccag caaagcagct acccgggtta tggtcagcag ccggcgccga
      421 gcagcagcag cggtagctat ggcagcagca gccaaagcag cagctacggt cagccgcaaa
      481 gcggtagcta cagccagcaa ccgagctacg gtggtcaaca gcaaagctac ggtcaacagc
      541 agagctataa cccgccgcaa ggttatggcc aacagaacca gtacaacagc tgactcgagc
      601 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      661 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      721 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      841 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      901 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      961 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1021 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1081 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1141 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1201 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     1261 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa
//
