LOCUS       sequence-444737-       11320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty, Gateway-compatible expression vector for transient 
            transformation of N. benthamiana leaves. Vector backbone encodes 
            C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S 
            terminator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Lawson AW, Flores-Ibarra A, Cao Y, An C, Neumann U, Gunkel M, Saur 
            IML, Chai J, Behrmann E, Schulze-Lefert P
  TITLE     The barley MLA13-AVRA13 heterodimer reveals principles for 
            immunoreceptor recognition of RNase-like powdery mildew effectors
  JOURNAL   bioRxiv 2024.07.14.603419
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.07.14.603419"
COMMENT     Sequence Label: pGWB402SC
FEATURES             Location/Qualifiers
     source          1..11320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1248..1877
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2306..3379
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3445..3639
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3875..3897)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3983..4123
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4038..4057
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4138..4155)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4309..4897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4389..4408)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5143..5934)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6455..6479
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6653..6832
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6685..6868
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6889..7683
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6943..6962)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7553..7572
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      8073..8325
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8699..9043
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8987..9007
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9060..9184
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(9209..9214,9215..9232,9233..9239)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9293..9952
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9359..9378)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10294..10599
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10478..10497
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10640..10764)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             10811..10834
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             10871..10894
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             10925..10951
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             10925..10951
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    11243..11267
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
       61 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
      121 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
      181 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
      241 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
      301 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
      361 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
      421 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
      481 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
      541 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
      601 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
      661 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
      721 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
      781 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
      841 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
      901 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
      961 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     1021 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc
     1081 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     1141 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     1201 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     1261 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
     1321 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     1381 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     1441 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     1501 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     1561 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     1621 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     1681 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     1741 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     1801 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     1861 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     1921 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     1981 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     2041 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     2101 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     2161 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     2221 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
     2281 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
     2341 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     2401 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     2461 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     2521 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     2581 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     2641 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     2701 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     2761 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     2821 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     2881 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     2941 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     3001 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     3061 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     3121 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     3181 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     3241 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     3301 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     3361 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     3421 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
     3481 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     3541 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     3601 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     3661 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     3721 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     3781 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     3841 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3901 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3961 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     4021 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     4081 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     4141 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4201 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4261 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4321 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4381 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4441 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4501 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4561 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4621 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4681 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4741 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4801 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4861 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4921 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcatgatat
     4981 atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac
     5041 ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taacgcttga gttaagccgc
     5101 gccgcgaagc ggcgtcggct tgaacgaatt tctagctaga cattatttgc cgactacctt
     5161 ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa
     5221 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
     5281 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
     5341 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
     5401 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
     5461 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
     5521 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
     5581 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
     5641 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
     5701 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
     5761 aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc
     5821 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
     5881 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc ccatgatgtt
     5941 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     6001 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     6061 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6121 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     6181 cgaacaggct tatgtccact gggttcgtgc ccgaattgat cacaggcagc aacgctctgt
     6241 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     6301 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     6361 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     6421 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     6481 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg
     6541 ccgaattagg ctgaatttat ttctgaattc atcatgagcg gagaattaag ggagtcacgt
     6601 tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca
     6661 acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag cacatacgtc
     6721 agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag caaatatttc
     6781 ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat tagagtctca
     6841 tattcactct caatccaaat aatctgcacc ggatctggat cgtttcgcat gattgaacaa
     6901 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6961 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     7021 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     7081 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     7141 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     7201 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     7261 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     7321 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     7381 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
     7441 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     7501 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     7561 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     7621 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     7681 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     7741 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7801 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc cacgggatct
     7861 ctgcggaaca ggcggtcgaa ggtgccgata tcattacgac agcaacggcc gacaagcaca
     7921 acgccacgat cctgagcgac aatatgatcg ggcccggcgt ccacatcaac ggcgtcggcg
     7981 gcgactgccc aggcaagacc gagatgcacc gcgatatctt gctgcgttcg gatattttcg
     8041 tggagttccc gccacagacc cggatgatcc ccgatcgttc aaacatttgg caataaagtt
     8101 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     8161 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     8221 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     8281 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttcagaaata
     8341 ggcgcgcccg gttcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
     8401 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
     8461 aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     8521 gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa
     8581 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatacatg gtggagcacg
     8641 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccag agggctattg
     8701 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     8761 gtcacttcat cgaaaggaca gtagaaaagg aagatggctt ctacaaatgc catcattgcg
     8821 ataaaggaaa ggctatcgtt caagatgcct ctaccgacag tggtcccaaa gatggacccc
     8881 cacccacgag gaacatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     8941 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     9001 acccttcctc tatataagga agttcatttc atttggagag gacctcgagg ctagcatcaa
     9061 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     9121 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     9181 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     9241 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     9301 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     9361 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     9421 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     9481 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     9541 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     9601 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     9661 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     9721 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     9781 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     9841 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     9901 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg
     9961 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
    10021 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
    10081 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
    10141 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
    10201 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
    10261 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
    10321 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
    10381 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
    10441 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
    10501 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
    10561 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
    10621 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
    10681 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
    10741 ttcagctttc ttgtacaaag tggttgatat caaggccgcc gacatcacca gcctgtacaa
    10801 gaaagtgggg tggagccatc cccagttcga gaagggcgga ggatctggcg gcggaagcgg
    10861 cggaggaagc tggtcccacc ctcagtttga gaaaggcacc gagctgggca gcaccatggc
    10921 cagctacccc tacgacgtgc ccgactacgc ctgatctaga gtccgcaaaa atcaccagtc
    10981 tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag
    11041 ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
    11101 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
    11161 aatccagtga ccgggcggcc gccaccgcgg tggaggggga tcagattgtc gtttcccgcc
    11221 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
    11281 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
//
