LOCUS       sequence-44474-e        7969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Mediouni S, Jablonski J, Paris JJ, Clementz MA, Thenin-Houssier S, 
            McLaughlin JP, Valente ST
  TITLE     Didehydro-cortistatin A inhibits HIV-1 Tat mediated 
            neuroinflammation and prevents potentiation of cocaine reward in Tat
            transgenic mice.
  JOURNAL   Curr HIV Res. 2015;13(1):64-79.
  PUBMED    25613133
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr HIV 
            Res."; date: "2015"; volume: "13(1)"; pages: "64-79"
COMMENT     Sequence Label: pcDNA3.1-hnRNPU-V5
FEATURES             Location/Qualifiers
     source          1..7969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3467..3508
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3518..3535
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3558..3575)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3564..3788
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3834..4262
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3921..3940)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4131..4152
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4271..4291)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4276..4605
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4456..4591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4518..4537
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4672..5466
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4726..4745)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5336..5355
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5642..5763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5679..5698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5733..5752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5812..5828)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5812..5828)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5825..5847)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5836..5852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5860..5866,5867..5884,5885..5890))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5905..5926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6043..6060)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6214..6802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6294..6313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6973..7764,7765..7833))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7596..7615
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7834..7938)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttatg agttcctcgc ctgttaatgt aaaaaagctg aaggtgtcgg agctgaaaga
      961 ggagctcaag aagcgacgcc tttctgacaa gggtctcaag gccgagctca tggagcgact
     1021 ccaggctgcg ctggacgacg aggaggccgg gggccgcccc gccatggagc ccgggaacgg
     1081 cagcctagac ctgggcgggg attccgctgg gcgctcggga gcaggcctcg agcaggaggc
     1141 cgcggccggc ggcgatgaag aggaggagga agaggaagag gaggaggaag gaatctccgc
     1201 tctggacggc gaccagatgg agctaggaga ggagaacggg gccgcggggg cggccgactc
     1261 gggcccgatg gaggaggagg aggccgcctc ggaagacgag aacggcgacg atcagggttt
     1321 ccaggaaggg gaagatgagc tcggggacga agaggaaggc gcgggcgacg agaacgggca
     1381 cggggagcag cagcctcaac cgccggcgac gcagcagcaa cagccccaac agcagcgcgg
     1441 ggccgccaag gaggccgcgg ggaagagcag cggccccacc tcgctgttcg cggtgacggt
     1501 ggcgccgccc ggggcgaggc agggccagca gcaggcggga ggtaagaaga aggcggaagg
     1561 cggcggaggc ggcggtcgcc ccggggctcc ggcggcgggg gacggcaaaa cagaacagaa
     1621 aggcggagat aaaaagaggg gtgttaaaag accacgagaa gatcatggcc gtggatattt
     1681 tgagtacatt gaagagaaca agtatagcag agccaaatct cctcagccac ctgttgaaga
     1741 agaagatgaa cacttcgatg acacagtggt ttgtcttgat acttataatt gtgatctaca
     1801 ttttaaaata tcaagagatc gtctcagtgc ttcttccctt acaatggaga gttttgcttt
     1861 tctttgggct ggaggaagag catcctatgg tgtgtcaaaa ggcaaagtgt gttttgagat
     1921 gaaggttaca gagaagatcc cagtaaggca tttatataca aaagatattg acatacatga
     1981 agttcgtatt ggctggtcac taactacaag tggaatgtta cttggtgaag aagaattttc
     2041 ttatgggtat tctctaaaag gaataaaaac atgcaactgt gagactgaag attatggaga
     2101 aaagtttgat gaaaatgatg tgattacatg ttttgctaac tttgaaagtg atgaagtaga
     2161 actctcgtat gctaagaatg gacaagatct tggcgttgcc ttcaaaatca gtaaggaagt
     2221 tcttgctgga cggccactgt tcccgcatgt tctctgccac aactgtgcag ttgaatttaa
     2281 ttttggtcag aaggaaaagc catattttcc aatacctgaa gagtatactt tcatccagaa
     2341 cgtcccctta gaggatcgag ttagaggacc aaaggggcct gaagagaaga aagattgtga
     2401 agttgtgatg atgattggct tgccaggagc tggaaaaact acctgggtta ctaaacatgc
     2461 agcagaaaat ccagggaaat ataacattct tggcacaaat actattatgg ataagatgat
     2521 ggtggcaggt tttaagaagc aaatggcaga tactggaaaa ctgaacacac tgttgcagag
     2581 agccccccag tgtcttggga aatttattga gattgctgcc cgaaagaagc gaaattttat
     2641 tctggatcag acaaatgtgt ctgctgctgc ccagaggaga aaaatgtgcc tgtttgcagg
     2701 cttccagcga aaagctgttg tagtttgccc aaaagatgaa gactataagc aaagaacaca
     2761 gaagaaagca gaagtagagg ggaaagacct accagaacat gcggtcctca aaatgaaagg
     2821 aaactttacc ctcccagagg tagctgagtg ctttgatgaa ataacctatg ttgaacttca
     2881 gaaggaagaa gcccaaaaac tcttggagca atataaggaa gaaagcaaaa aggctcttcc
     2941 accagaaaag aaacagaaca ctggctcaaa gaaaagcaat aaaaataaga gtggcaagaa
     3001 ccagtttaac agaggtggtg gccatagagg acgtggagga ttcaatatgc gtggtggaaa
     3061 tttcagagga ggagcccctg ggaatcgtgg cggatataat aggaggggca acatgccaca
     3121 gagaggtggt ggcggtggag gaagtggtgg aatcggctat ccataccctc gtgcccctgt
     3181 ttttcctggc cgtggtagtt actcaaacag agggaactac aacagaggtg gaatgcccaa
     3241 cagagggaac tacaaccaga acttcagagg acgaggaaac aatcgtggct acaaaaatca
     3301 atctcagggc tacaaccagt ggcagcaggg tcaattctgg ggtcagaagc catggagtca
     3361 gcattatcac caaggatatt atggatccac tagtccagtg tggtggaatt ctgcagatat
     3421 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     3481 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
     3541 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3601 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3661 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3721 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     3781 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     3841 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3901 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3961 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4021 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4081 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4141 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4201 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4261 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4321 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4381 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4441 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4501 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4561 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4621 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4681 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4741 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4801 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     4861 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4921 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4981 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5041 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5101 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5161 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5221 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5281 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5341 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5401 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5461 ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc
     5521 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5581 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5641 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5761 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     5821 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5881 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5941 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6001 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6061 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7261 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7561 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7801 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7861 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7921 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
