LOCUS       sequence-444743-       13240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3 subunits of photosynthetic reaction center from R. 
            sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13240)
  TITLE     Reaction center amber suppression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIND4-RC-C2-M210TAG+M252V-oCNF
FEATURES             Location/Qualifiers
     source          1..13240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      485..571
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(742..1557)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1465..1484
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1549..1568
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5329..5917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5818..5837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6071..6088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(6426..7508)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7470..7489
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7509..7586)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     7778..7796
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    7842..7858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7875..7891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7910..7921
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             8708..8725
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      10546..10640
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(10750..10769)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     12867..12889
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12881..12898
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12882..12898
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
       61 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
      121 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
      181 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
      241 atatggacaa cttcttcgcc cccgttttca ccatgcatgg gcaaatatta tacgcaaggc
      301 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
      361 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
      421 tttttttaag gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag
      481 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      541 gtcggtgaac gctctcctga gtaggacaaa tccgccgcta ggagcttgcg gccctggcgt
      601 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      661 tgcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta
      721 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
      781 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
      841 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
      901 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
      961 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aacagcttat gcatttcttt
     1021 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     1081 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     1141 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     1201 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
     1261 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     1321 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     1381 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     1441 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     1501 catgttggaa tttaatcgcg gcttcgagca agacgtttcc cgttgaatat ggctcataac
     1561 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     1621 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
     1681 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
     1741 ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg
     1801 tggctccctc gaggctccgc gccctcgatc cgaccgcccc ccacaaggct ggggcggatc
     1861 gggggcgcac ggcctgaagg gtgcagggga accctgccgc acgcaaactc gcagagtttg
     1921 catagtgtgc gccattcaga taccttcatg gcaggcccca ccgtggattt tgctgtcatg
     1981 gttcgattca tcacgacgcc cgcaacgccg actgcacagc ggcccgcacc acgaacgcct
     2041 gcgcagcagg aagctgcggc tgaactgaag gctgcgcgaa ctgccgaagc agcccgccgc
     2101 ctcgacaccc ggcgcaaggt ggtgctcggg ggcgcgctcc tggcactggc agaacgtgat
     2161 gcagcagcag ccgcgatgct ggcccggctg aagcagatgc tgacccgccc agcagatcgg
     2221 aagctgttcg cggatctacc cgatggctga cggtgcaatc ttccacctcg atgtgcgcac
     2281 ggtcagccgg tcagatggcc gctcggctgt cgccgctgcc gcctatcggt cggccagccg
     2341 tctgcacgat gcacgcaccg gcctgaccca cgacttcgcg cgcaagcggg gcgtcttgac
     2401 aaccttcatc gccgccccgg acggctgcga ctggatcaca gacagggaca cgctctggaa
     2461 cgcggctgag gcggcagaga agcgcaagaa cagcaccgtg gcccgtgagt ggctggtggc
     2521 cctccctgac gctctggatg ctacacagcg ggccgacctg gcgcgggcgc tggcggtcga
     2581 actggcgcag cggttcgggg tggccgtgga tgtggcaatc cacgcagctt cgggcgaggg
     2641 cgaccagcgg aaccatcacg cgcatttgct gaccacgacg cgcaccgcag gcccggacgg
     2701 gctgggcgac aagacccgcg ttctggatgc agccaaaacg gggggcgcag aaatctacgc
     2761 catgcgggct tggtgggcgg gcacggtcaa cgatgcactc gcggccgcac aaagctcggc
     2821 ccgcgtcgat caccgccgca tagccgtgat cgccgccgat gcgcgggccg aggccgaggc
     2881 gctggagaaa caggccaaag ccgtcgaaac cctgaacgcc aagcccgccg acgccggggg
     2941 gctgtggaag ggccttggca gcgccgcccg cgcaatatgg gaaggcggca tgaccgccct
     3001 caaatcgacc gccgagacag ccaacgagct gcgggagaag gccaacgagc tgcgacagaa
     3061 ggcagcccgc tacacccgcg ccaccgaacc tcaccacggc cccgtcctga cggccttctt
     3121 gcggcgcatg gctcccgtct gggcggagca ggccaaggcc gaggaagccc ggagagccgc
     3181 gcaggaggcc gcacagcgcg cactgatggc cgagagggct gaacaggccc ggaaggccaa
     3241 ggaagcctct gaggccgctc gccgggccgc tacggcagcg cgagaggccg aggaagcccg
     3301 cgaacgctgc gcccgcgagg tgttgccgct catccgccgc gcccggcacg acccgatcac
     3361 cgccgaagtg atcaagcggg agggcatcga cctcgaccgc cccgaccaac aggtggcccg
     3421 cgacccgatc tggctcatgg ccggacgcca agatcaggcg cacccggtct ggctgatcgt
     3481 ccacgcctac accgccgcgc gcgacaggca cgcgaacctg ctggcgagaa tcgaagccaa
     3541 aaccgcagcc cagatcgccg ctgccgccaa accaaagccc aagcctgcca gtcccgcccc
     3601 gaccgaaccc gaacccgatc cagacccacc gccccggcgg gattggggcg ggccgtctgg
     3661 accctgatcc accccccatt ctcagcaact ccgagccagc cagaggcgct ggctcggggc
     3721 atctggaccc ccttcctcac cccaaatttt cacgaaaaag gggcgcagcc cttcttgttc
     3781 aataagttct ataagttcag gcgaaaatcg cctttcattt tcaatggctt gcgcgcctat
     3841 cggggagaaa tggggccgca aaagggagaa atggggccgc aaaagggaga aatggggccg
     3901 atagcggctg ttgacagggg gatattttgc cccctacatt tctccccatg acaaagacca
     3961 cgcaaatcgc cgctgatcga gcctatgacg aggccaaaac cgtgctgccc gcagaggtgg
     4021 cgcggggggt ctacatgcgc aacgccccca gcctcgccgc cctcaagctg atgcacctgc
     4081 tgatcggtaa ggcgggcggg cgtatggccg aacccattcg gcacgaaatc cgcctctcag
     4141 acatccgcaa gatcgacggg atgaagaacc atgacagggc cagcctcacc ccccttttcg
     4201 cggaattgtc ggcggctgtc ctgacccatg atgaccccga aaaaatggtc gtgaccattg
     4261 gcggcttgct ggacttcgcc cgcatcgact accgccacga agtcagcaac gatctgctgg
     4321 tgtcgtggta ttttggcggg gccttccgcg agatggccga gaagtcctgc cactgggcga
     4381 tcctcgaccg gcagaccgtt tttgccctgt ccagcaagta ttcgatcctg ctgttccagc
     4441 acatcgccag cctcgtgaac ctcgatcacg tcgccagcaa gaccttcacc gtggccgaac
     4501 tgcgggccgt gctaggggtg ccgcacggaa agatggagcg gtggaacgac gtgaaccgct
     4561 ttgcgctgac gcccgcgatt gccgagatca accagacctc gcgcctgatc ctgaccgcga
     4621 ccccgaacaa gatcgggcgg acggtcgcca gcgtgacaat cacgtgggcg gagaagcctc
     4681 ccgagggcaa gcaggaagcc aagcgcgaac tggaccgccc caaggtgggc cgagaggcgc
     4741 ggcgcaacgg ctcagccgag accgtggcgc gggccttccc cagctcgggc agcatcgagt
     4801 tcgatgacca ctggaaggcc ctcaagcggg cggcgggctg caacatggac aacaccatga
     4861 ttgccgagaa gttccgggcg tggtgcgcca gcaaggggct ttccctcgac ggccgaaaca
     4921 tcgagcaggc gttcagcaac ttctgcgcca cagtgggcaa ggtctgaggc cgcgcccgct
     4981 gccagtgacc cgccgccgcc gaccgtgagc gggccgcagc ccttccagcg ccgcccaaga
     5041 gggcggcaag ccccggtctg ccccctcgtc gcccgcaaag cgggcagggg gcagaccggg
     5101 gcgctggaac ctttgggctg ctttgacggc cagacagccc ccgccgcgca agcgcgccat
     5161 tcccccaccc gtggccgggc gggggaatgg taaagcatgt gggggcagtc cagccgtcaa
     5221 tccgcgctcc gcaccacggc gcctcgagca acaccttctt cacgaggcag acctctcgac
     5281 ggagttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5341 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5401 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5461 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5521 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5581 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5641 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5701 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5761 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5821 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5881 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5941 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6001 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6061 gaccgagcgc agcgagtcag tgagcgagga agcggaagaa gctcgcacat tcagcagcgt
     6121 ttttcagcgc gttttcgatc aacgtttcaa tgttggtatc aacaccaggt ttaactttga
     6181 acttatcggc actgacggtt actgattttg aacttttgct ttgccacgga acggtctgcg
     6241 ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgcca atacgcaaac
     6301 cgcctctccc cgcgcgttgg ccgattcatt aatgcagggc cgcgcgaatt cgagctcggt
     6361 accgacgtag cccagcgcgt cggccagctt gcaattcgcg ctaacttaca ttaattgcgt
     6421 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6481 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     6541 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     6601 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     6661 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
     6721 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     6781 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     6841 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     6901 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     6961 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     7021 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     7081 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     7141 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     7201 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     7261 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     7321 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     7381 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     7441 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     7501 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     7561 aaggttttgc accattcgat ggtgtcaacg taaatgcatg ccgcttcgcc ttcgcgcgcg
     7621 aattgcaggt accatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7681 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7741 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7801 tcgtcttcac ctcgagaaaa tttatcaaaa agagtgttga cttgtgagcg gataacaatg
     7861 atacttagat tcaattgtga gcggataaca atttcacaca tctagaatta aagaggagaa
     7921 attaaccatg gttggtgtga ctgcttttgg aaacttcgat ctggcgtcgc tggcgatcta
     7981 tagcttctgg atcttcctcg cgggcctgat ctactacctc cagaccgaga acatgcgcga
     8041 gggctatccg ctggagaacg aggacggcac cccggccgcg aaccagggcc cgttcccgct
     8101 gccgaagccc aagaccttca tcctgcccca cggccgcggc acgctgaccg tacccggccc
     8161 ggaaagcgaa gaccgcccga tcgcgctcgc gcggacggcc gtctcggaag gcttcccgca
     8221 tgcgcccacg ggcgacccga tgaaggacgg cgtcggcccg gcctcgtggg ttgcgcgccg
     8281 tgacctgccc gaactcgacg ggcacggcca caacaagatc aagccgatga aggccgctgc
     8341 cggcttccac gtctcggccg gcaagaaccc gatcggcctg cccgtccgcg gctgcgatct
     8401 cgagatcgcg ggcaaggtcg tggacatctg ggtcgacatc cccgagcaga tggcccgctt
     8461 cctcgaggtc gaactcaagg acggctcgac ccgcctcctg ccgatgcaga tggtcaaggt
     8521 ccagtcgaac cgcgtccatg tgaacgcgct ctcgtccgac ctgttcgcgg gcatcccgac
     8581 gatcaagtcc ccgaccgagg tcacgctcct cgaagaggac aagatctgcg gctacgtcgc
     8641 cggcggcctg atgtatgccg cgccgaagcg caagtcggtc gtggcggcga tgctggccga
     8701 atacgtgcac caccaccacc accactgagg atccgttggg ccgactgcaa gcggagaggg
     8761 aagcatggca ctgctcagct tcgagcgaaa atatcgcgtg ccggggggca cgctggtcgg
     8821 cggaaacctg ttcgacttct gggtcggccc tttctatgtc ggcttcttcg gggttgcgac
     8881 gtttttcttc gcggccctgg gtatcattct gattgcctgg agtgccgtac tccagggtac
     8941 ctggaacccc caactcatct ctgtctaccc gccggccctt gaatatggcc tgggaggtgc
     9001 acccctcgca aaaggcgggc tgtggcagat catcacgatc tgcgccactg gtgccttcgt
     9061 cagctgggcg ctgcgcgaag tcgaaatctg ccgtaagctg ggcatcgggt accacatccc
     9121 gttcgccttc gcgttcgcca tcctggccta cctgacgctg gtgctgttcc gcccggtgat
     9181 gatgggcgcc tggggctatg ccttccccta cgggatctgg acgcacctcg actgggtgtc
     9241 gaacacgggc tacacctacg gcaacttcca ctacaaccct gcccacatga tcgccatctc
     9301 gttcttcttc acgaacgcgc tggctctggc gctgcacggc gcccttgtgc tctccgcggc
     9361 caaccccgag aagggcaagg aaatgcggac gccggatcac gaggatacgt tcttccgcga
     9421 tctggtcggc tactcgatcg ggacgctcgg catccaccgc ctcggcctgc tgctctcgct
     9481 gagcgccgtc ttcttcagcg ccctctgcat gatcattacc ggcaccatct ggttcgatca
     9541 gtgggtcgac tggtggcaat ggtgggtgaa gctgccgtgg tgggcgaaca tcccgggagg
     9601 catcaatggc tgagtatcag aacatcttct cccaggtcca ggtccgcgga ccggccgacc
     9661 tggggatgac cgaagacgtc aacctggcca accgttcggg cgtcggtccc ttctcgaccc
     9721 tgctcggctg gttcggcaac gcccagctcg gcccgatcta tctcggctcg ctcggcgtcc
     9781 tgtccctctt ctcgggcctg atgtggttct tcaccatcgg gatctggttc tggtatcagg
     9841 cgggctggaa cccggccgtc ttcctgcgcg acctgttctt cttctcgctc gagccgccgg
     9901 cacccgaata cggtctgtcc ttcgcggctc cgctgaagga aggcgggctg tggctgatcg
     9961 cgtcgttctt catgttcgtc gcggtctggt cctggtgggg ccgcacctat ctccgcgctc
    10021 aggcgctggg catgggcaag cacaccgcct gggcgttcct ctcggccatc tggctgtgga
    10081 tggtgctggg cttcatccgt ccgatcctca tggggtcctg gtcggaagcg gttccctacg
    10141 gcatcttctc gcacctcgac tggacgaaca acttctcgct cgtccacggc aacctgttct
    10201 acaacccctt ccacggtctc tcgatcgcct tcctctaggg gtcggccctg ctcttcgcga
    10261 tgcacggtgc gaccatcctc gcggtctccc gcttcggcgg cgagcgcgag ctggagcaga
    10321 tcgccgaccg cgggacggca gcggagcggg ccgccctctt cgtgcgctgg accatgggtt
    10381 tcaacgccac gatggaaggc atccaccgct gggccatctg gatggcggtc ctcgtgaccc
    10441 tcaccggcgg catcggcatc ctgctctcgg gcacggtcgt ggacaactgg tacgtctggg
    10501 gccagaacca cggcatggcg ccgctgaact gaacatatga gcttggactc ctgttgatag
    10561 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
    10621 cgttttttat tggtgagaat ccaagctagc ttggcgagat tttcaggagc taaggaagct
    10681 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
    10741 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
    10801 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
    10861 attcacattc ttgcccgcct gatgaatgct catgctagcg agctcgccgc tcatggcgtt
    10921 ctgttgcccg tctcactggt gaaaagaaaa acaaccctgg cgccgcttct ttgagcgaac
    10981 gatcaaaaat aagtggcgcc ccatcaaatt gttacggagc ccaaaaaatc cgcttgcgcc
    11041 cggggccgtc tgctcctaga aaccgcttca ccgagacgaa gaccggcagc gccggacgga
    11101 gacgagggag cggatgacag aaacgtcggc cgcgacaatt gaagatgagg cggacgggat
    11161 cgctggttgt ctgcatcaac ggaggtcccc catggataaa aaaccactaa acactctgat
    11221 atctgcaacc gggctctgga tgtccaggac cggaacaatt cataaaataa aacaccacga
    11281 agtctctcga agcaaaatct atattgaaat ggcatgcgga gaccaccttg ttgtaaacaa
    11341 ctccaggagc agcaggactg caagagcgct caggcaccac aaatacagga agacctgcaa
    11401 acgctgcagg gtttcggatg aggatctcaa taagttcctc acaaaggcaa acgaagacca
    11461 gacaagcgta aaagtcaagg tcgtttctgc ccctaccaga acgaaaaagg caatgccaaa
    11521 atccgttgcg agagccccga aacctcttga gaatacagaa gcggcacagg ctcaaccttc
    11581 tggatctaaa ttttcacctg cgataccggt ttccacccaa gagtcagttt ctgtcccggc
    11641 atctgtttca acatcaatat caagcatttc tacaggagca actgcatccg cactggtaaa
    11701 agggaatacg aaccccatta catccatgtc tgcccctgtt caggcaagtg cccccgcact
    11761 tacgaagagc cagactgaca ggcttgaagt cctgttaaac ccaaaagatg agatttccct
    11821 gaattccggc aagcctttca gggagcttga gtccgaattg ctctctcgca gaaaaaaaga
    11881 cctgcagcag atctacgcgg aagaaaggga gaattatctg gggaaactcg agcgtgaaat
    11941 taccaggttc tttgtggaca ggggttttct ggaaataaaa tccccgatcc tgatccctct
    12001 tgagtatatc gaaaggatgg gcattgataa tgataccgaa ctttcaaaac agatcttcag
    12061 ggttgacaag aacttctgcc tgagacccat gcttgctcca aacctttaca actacctgcg
    12121 caagcttgac agggccctgc ctgatccaat aaaaattttt gaaataggcc catgctacag
    12181 aaaagagtcc gacggcaaag aacacctcga agagtttacc atgctggcct tcgcgcagat
    12241 gggatcggga tgcacacggg aaaatcttga aagcataatt acggacttcc tgaaccacct
    12301 gggaattgat ttcaagatcg taggcgattc ctgcatggtc tatggggata cccttgatgt
    12361 aatgcacgga gacctggaac tttcctctgc agtagtcgga cccataccgc ttgaccggga
    12421 atggggtatt gataaaccct ggataggggc aggtttcggg ctcgaacgcc ttctaaaggt
    12481 taaacacgac tttaaaaata tcaagagagc tgcaaggtcc gagtcttact ataacgggat
    12541 ttctaccaac ctgtaagatc cggtggatga ccttttgaat gacctttaat agattatatt
    12601 actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt ttcacaaaac
    12661 ggtttacaag cataaagctt gctcaatcaa tcacccatca gccctgaggt cgggctcctt
    12721 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
    12781 cctaatgcag gagtcgcata agggagagcg tctggcgaaa gggggatgtg ctgcaaggcg
    12841 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    12901 caagcttaaa aaaaatcctt agctttcgct aaggtggcgg aaaccccggg aatctaaccc
    12961 ggctgaacgg atttagagtc cattcgatct acatgatcag gtttcccgtt acacagacaa
    13021 ccagcgatcc cgtccgcctc atcttcaatt gtcgcggccg acgtttctgt catccgctcc
    13081 ctcgtctccg tccggcgctg ccggtcttcg tctcggtgaa gcggtttcta ggagcagacg
    13141 gccccgggcg caagcggatt ttttgggctc cgtaacaatt ttcactctag agtccggaag
    13201 tactatttcg aatttcgtat ggcaatgaaa gacggtgagc
//
