LOCUS       sequence-444763-        6494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of MAG Transposon with copGFP mammalian expression 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-MAG[EF1-alpha-copGFP]
FEATURES             Location/Qualifiers
     source          1..6494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1320..1337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1320..1336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1346..1364)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1432..1536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1537..1605,1606..2397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1755..1774)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2568..3156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3057..3076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3310..3327
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3339..3355
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3339..3355
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3899..3980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3936..3955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3990..4009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(4023..4688)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             complement(4023..4688)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     promoter        complement(4730..5908)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4739..5677
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4762..4782)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ctcagtcatt tcatttcttc atgtttgagc cgattttttc tcccgtaaat gccttgaatc
       61 agcctattta gaccgtttct tcgccattta aggcgttatc cccagttttt agtgagatct
      121 ctcccactga cgtatcattt ggtccgcccg aaacaggttg gccagcgtga ataacatcgc
      181 cagttggtta tcgtttttca gcaacccctt gtatctggct ttcacgaagc cgaactgtcg
      241 cttgatgatg cgaaatgggt gctccaccct ggcccggatg ctggctttca tgtattcgat
      301 gttgatggcc gttttgttct tgcgtggatg ctgtttcaag gttcttacct tgccggggcg
      361 ctcggcgatc agccagtcca catccacctc ggccagctcc tcgcgctgtg gcgccccttg
      421 gtagccggca tcggctgaga caaattgctc ctctccatgc agcagattac ccagctgatt
      481 gaggtcatgc tcgttggccg cggtggtgac caggctgtgg gtcaggccac tcttggcatc
      541 gacaccaatg tgggccttca tgccaaagtg ccactgattg cctttcttgg tctgatgcat
      601 ctccggatcg cgttgctgct ctttgttctt ggtcgagctg ggtgcctcaa tgatggtggc
      661 atcgaccaag gtgccttgag tcatcatgac gcctgcttcg gccagccagc gattgatggt
      721 cttgaacaat tggcgggcca gttgatgctg ctccagcagg tggcggaaat tcatgatggt
      781 ggtgcggtcc ggcaaggcgc tatccaggga taaccgggca aacagacgca tggaggcgat
      841 ttcgtacaga gcatcttcca tcgcgccatc gctcaggttg taccaatgct gcatgcagtg
      901 aatgcgtagc atggtttcca gcggataagg tcgccggcca ttaccagcct tggggtaaaa
      961 cggctcgatg acttccacca tgttttgcca tggcagaatc tgctccatgc gggacaagaa
     1021 aatctctttt ctggtctgac ggcgcttact gctgaattca ctgtcggcga aggtaagttg
     1081 atgactcatg atgaaccctg ttctatggct ccagatgaca aacatgatct catatcaggg
     1141 acttgttcgc accttcccta atagtgcttt catgaaagtt tggtaaagat tgattcactt
     1201 taagtcatga aatatgataa gtgaataata aattgataag tggtcctatt gatttgtccg
     1261 acactgtata caattgagag acgcaagaca ctgcggatcg agacgcattg cgtggttcga
     1321 ctggccgtcg ttttacaacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1381 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1441 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1501 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1561 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1621 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1681 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1741 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1801 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1861 aagcatctta cggatggcat gacagtaaga gaattatgta gtgctgccat aaccatgagt
     1921 gataacacgg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1981 tttttgcgca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2041 gaagccatac caaacgacga gcgtgatacc acgatgcctg tagcgatggc aacaacgttg
     2101 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2161 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2221 attgctgata aatctggagc cggtgagcgt gggtcccgcg gtattattgc agcactgggg
     2281 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2341 gatgaacgaa agagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2401 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2461 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2521 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2581 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2641 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2701 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2761 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2821 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2881 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2941 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3001 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3061 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3121 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3181 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3241 tctgtgggta accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3301 accgagcgca gcgagtcagt gagcgaggaa gcggcacaca ggaaacagct atgaccgtct
     3361 cggttggcag tgactccgtc tctgggccct atacagtgtc ggacaaatca ataggaccac
     3421 ttaataatgt tttattcact tatcacattt cattacctaa agtgaatcaa tatttaccaa
     3481 attttcatga aagcactttt cggatgttgg cttaaagtct gctttgaaag tttgttgata
     3541 agttaacaaa caaaaaagtt attataagaa atgttgaaaa aaccccaaaa atcatgcgac
     3601 aaaataatag gaccagtata gaaaatttta ttattttaac catttatcat tattgcctat
     3661 aaataattac agctccggct tttaattcgc aatgtggcca gcccgtacat ttgaaaaact
     3721 aaagaaaata acaaaacagc cttatcctgg gctaacttgg tagcatctcg agggcgcgcc
     3781 ctcgcccttt ggtctccccc tcttaagtac cacatttgta gaggttttac ttgctttaaa
     3841 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatcg agctcgagat
     4021 ctggcgaagg cgatgggggt cttgaaggcg tgctggtact ccacgatgcc cagctcggtg
     4081 ttgctgtgca gctcctccac gcggcggaag gcgaacatgg ggcccccgtt ctgcaggatg
     4141 ctggggtgga tggcgctctt gaagtgcatg tggctgtcca ccacgaagct gtagtagccg
     4201 ccgtcgcgca ggctgaaggt gcgggcgaag ctgcccacca gcacgttatc gcccatgggg
     4261 tgcaggtgct ccacggtggc gttgctgcgg atgatcttgt cggtgaagat cacgctgtcc
     4321 tcggggaagc cggtgcccac caccttgaag tcgccgatca cgcggccggc ctcgtagcgg
     4381 tagctgaagc tcacgtgcag cacgccgccg tcctcgtact tctcgatgcg ggtgttggtg
     4441 tagccgccgt tgttgatggc gtgcaggaag gggttctcgt agccgctggg gtaggtgccg
     4501 aagtggtaga agccgtagcc catcacgtgg ctcagcaggt aggggctgaa ggtcagggcg
     4561 cctttggtgc tcttcatctt gttggtcatg cggccctgct cgggggtgcc ctctccgccg
     4621 cccaccagct cgaactccac gccgttcagg gtgccggtga tgcggcactc gatcttcatg
     4681 gcgggcatgg tggcgaccgg tagcgctagc ggcttcggta ccggatccct cacgacacct
     4741 gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac caagatccaa
     4801 actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc ctaacttcag
     4861 tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc caacctaaag
     4921 acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc cggtactcag
     4981 tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt gtgggtgact
     5041 cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca gcagggccgg
     5101 gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt gccgcccagg
     5161 gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcga gcttgagact
     5221 acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct
     5281 gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat
     5341 cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa
     5401 aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga
     5461 ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg
     5521 ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc tcgggatcaa
     5581 gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa
     5641 agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg gcaaacccgt
     5701 tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg
     5761 gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct
     5821 aggcaccggg tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc
     5881 gctctgccca ctgacgggca ccggagccac tagtcaataa tcaatgcctc tagcaccggt
     5941 cgttggaatt ccttcaatca agcgcttgaa gtagtgcacc caaaaataca cgagctaatg
     6001 gatcgtgtga agtgttctag gcaacggctt agaacacagt gtaagcgatc cagcgctgtg
     6061 acgcgctttt atacccatag acgaaggtac tcaccactat ttcgtctctc agtgcaaagg
     6121 gagctcaaaa cccttaagcg agtgccgtaa tattcttgca ttagtaaatt agagcatgtt
     6181 aattaaagac acagcgaatt gccactgcgc taagcgtttc tacaaaaaaa atgaatacgc
     6241 attagttcac taatttaagt taagttatcc gacaactcaa taacatcaaa aactgtgttt
     6301 taacaataat aaatgataat tattttaaaa ttttctgaac tggtcctatt attttggcgc
     6361 atgatttttg ggttattttt acatttctta tcataacttt tttgtttctt aacttttcac
     6421 caaactttaa aaacattctt aaagtaaaca ttctaaggaa ggtgcgaata agcggggaaa
     6481 ttcttctcgg ctga
//
