LOCUS       sequence-444765-        5039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of SB100X Transposon with copGFP mammalian expression 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-SB100X[EF1-alpha-copGFP]
FEATURES             Location/Qualifiers
     source          1..5039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(303..1481)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          312..1250
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(335..355)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(1942..1959)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1942..1958)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1968..1986)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2054..2158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2159..2227,2228..3019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2377..2396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3190..3778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3679..3698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3932..3949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3961..3977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3961..3977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4511..4592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4548..4567)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4602..4621
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(4635..5039,1..261))
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             complement(join(4635..5039,1..261))
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
ORIGIN
        1 gcgggtgttg gtgtagccgc cgttgttgat ggcgtgcagg aaggggttct cgtagccgct
       61 ggggtaggtg ccgaagtggt agaagccgta gcccatcacg tggctcagca ggtaggggct
      121 gaaggtcagg gcgcctttgg tgctcttcat cttgttggtc atgcggccct gctcgggggt
      181 gccctctccg ccgcccacca gctcgaactc cacgccgttc agggtgccgg tgatgcggca
      241 ctcgatcttc atggcgggca tggtggcgac cggtagcgct agcggcttcg gtaccggatc
      301 cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg
      361 aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct
      421 ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc
      481 ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc
      541 gcccggtact cagtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt
      601 tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctccct
      661 gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc
      721 cttgccgccc agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg
      781 cgagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc
      841 gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata
      901 ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca
      961 gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga
     1021 aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca
     1081 cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga
     1141 agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg
     1201 ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg
     1261 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
     1321 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg
     1381 cgccaccttc tctaggcacc gggtcaattg ccgacccctc cccccaactt ctcggggact
     1441 gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc cactagtcaa taatcaatgc
     1501 ctctagcacc ggtattccac agaattccat cacaaagctc tgacctcaat cctatagaaa
     1561 ggaggaatga gccaaaattc acccaactta ttgtgggaag cttctaaagc catgacatca
     1621 ttttctggaa ttttccaagc tgtttaaagg cacagtcaac ttagtgtatg taaacttctg
     1681 acccactgga attgtgatac agtgaattat aagtgaaata atctgtctgt aaacaattgt
     1741 tggaaaaatg acttgtgtca tgcacaaagt agatgtccta actgacttgc caaaactatt
     1801 gtttgttaac aagaaatttg tggagtagtt gaaaaacgag ttttaatgac tccaacttaa
     1861 gtgtatgtaa acttccgact tcaactgtat agactagtag agacgcaaga cactgcggat
     1921 cgagacgcat tgcgtggttc gactggccgt cgttttacaa cgaaagggcc tcgtgatacg
     1981 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2161 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2221 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2281 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2341 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2401 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2461 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2521 tagtgctgcc ataaccatga gtgataacac ggcggccaac ttacttctga caacgatcgg
     2581 aggaccgaag gagctaaccg cttttttgcg caacatgggg gatcatgtaa ctcgccttga
     2641 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgata ccacgatgcc
     2701 tgtagcgatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2761 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2821 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcccg
     2881 cggtattatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2941 gacggggagt caggcaacta tggatgaacg aaagagacag atcgctgaga taggtgcctc
     3001 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3841 ctttcctgcg ttatcccctg attctgtggg taaccgtatt accgcctttg agtgagctga
     3901 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggcaca
     3961 caggaaacag ctatgaccgt ctcggttggc agtgactccg tctctgggcc cctatacagt
     4021 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
     4081 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
     4141 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
     4201 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcaattagta tttggtagca
     4261 ttgcctttaa attgtttaac ttgggtcaaa catttcgagt agccttccac aagcttctga
     4321 tagactaatt gacatcattt gagtcaattg gaggtgtacc tgtggatgta tttcaaggct
     4381 cgagggcgcg ccctcgccct ttggtctccc cctcttaagt accacatttg tagaggtttt
     4441 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4501 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4621 cgagctcgag atctggcgaa ggcgatgggg gtcttgaagg cgtgctggta ctccacgatg
     4681 cccagctcgg tgttgctgtg cagctcctcc acgcggcgga aggcgaacat ggggcccccg
     4741 ttctgcagga tgctggggtg gatggcgctc ttgaagtgca tgtggctgtc caccacgaag
     4801 ctgtagtagc cgccgtcgcg caggctgaag gtgcgggcga agctgcccac cagcacgtta
     4861 tcgcccatgg ggtgcaggtg ctccacggtg gcgttgctgc ggatgatctt gtcggtgaag
     4921 atcacgctgt cctcggggaa gccggtgccc accaccttga agtcgccgat cacgcggccg
     4981 gcctcgtagc ggtagctgaa gctcacgtgc agcacgccgc cgtcctcgta cttctcgat
//
