LOCUS       sequence-444786-       12696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Csy4 with 5' and 3' targets (II) and without Cas for Lotus callus 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Bircheneder M, Schreiber T, Tissier A, Parniske M
  TITLE     A quantitative assay for the efficiency of RNA-guided genome editing
            in plants.
  JOURNAL   Plant J. 2024 Sep;119(5):2564-2577. doi: 10.1111/tpj.16931. Epub 
            2024 Jul 20.
  PUBMED    39032106
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16931";
            journalName: "Plant J"; date: "2024-09"; volume: "119"; issue: "5"; 
            pages: "2564-2577"
COMMENT     Sequence Label: pMB21
FEATURES             Location/Qualifiers
     source          1..12696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..158
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     73..92
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(173..190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(344..932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(424..443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1002..1021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1003..1021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1026..1042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1026..1042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1144..1163)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1155..1964
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1222..1241)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2220..2244
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2371..2550
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2403..2586
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2607..3632
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             5128..5145
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    5156..5175
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             join(5215..5217,5218..5220,5221..5931)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5260..5281)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5521..5540)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5868..5889
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5944..5964
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5993..6245
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      8342..8590
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8654..8678
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9979..10608
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11037..12110
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12176..12370
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12606..12628)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
       61 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
      121 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
      181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      961 ctgacgctca taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     1021 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1081 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     1141 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1201 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1261 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1321 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1381 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1441 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1501 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1561 attgtccttt taacagcgat cgcgtatttc gtcttgctca ggcgcaatca cgaatgaata
     1621 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1681 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1741 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1801 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     1861 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     1921 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     1981 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     2041 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agacccctgc cggtaacatg
     2101 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     2161 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     2221 ggcaggatat attgtggtgt aaacaaattg acgcttagac ggattacggg tctcagcggt
     2281 acggcgggat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     2341 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     2401 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     2461 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     2521 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
     2581 tctgcaccgg atcttctgga agcaccatga aaaagcctga actcaccgcg acgtctgtcg
     2641 agaagtttct gatcgaaaag ttcgacagcg tgtccgacct gatgcagctc tcggagggcg
     2701 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     2761 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     2821 tcccgattcc ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct
     2881 cccgccgttc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     2941 tacaaccggt cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     3001 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     3061 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     3121 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     3181 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     3241 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     3301 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     3361 ggcatccgga gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg
     3421 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     3481 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     3541 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     3601 gccccagcac tcgtccgagg gcaaagaaat agaagggcta agacctaagc tagcctagaa
     3661 tccggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct
     3721 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
     3781 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     3841 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgaccttg agtgacctaa
     3901 gctagcctag tgtcgggcta gataattagg ctaaactatc cttaaggatt gtagataatt
     3961 aggctaaact atccttaagg tgtcgcgggg agagaggatt ttgaggaaat aattaattga
     4021 attacttgta ttattgataa agtaatttag ataagttgtt agtacaactt attgcaacta
     4081 tggatagaca aaaatcactt attttaaggg gagaagtata taacaactta ttataaaatt
     4141 tctgagcagc cggcctcctc caatcaatca tagagagtgg agcccattct ggaaaaccaa
     4201 ggaaccccca acttgcactg gtgcggtggc ccaattcaaa aaaaacgggg cccagcaaag
     4261 taacgccgtt gtaacgattt atcaatccaa actcaaaagg cggcgagatg cgtttaactc
     4321 cgtgaaatta acaaaccgcc aacaacttgc aatttgcaac taccgtttcc aagaagaact
     4381 caaccacaca acgtatccta tcccaaacca cacgcaacta gtgacgcgtc ataaggacac
     4441 gtgtcacaat ttgactggtt aataatttca ccgcttttgc tataaattac ctccaatccc
     4501 cttagcttct tcacaattca gttcccaacc ctaacaattc ttgttcatat cgcttctctc
     4561 tactttcaag gtatgatcca atttctctct tctttctctg taatcctttc gttgagtttt
     4621 gtttccgatc aatcataggt agttttcttg tttcgaagca tgagatctag gaattttttg
     4681 tgattttcca aaattgagat cggtttgaaa ttgaatttta cagcttgaat ctcagatctt
     4741 gttttatcaa tgttttcgat ggcttgcgat gtagatctat gataattgtg gttcagtttt
     4801 gttaggaatc gatttcggtt tagcaattgc agattaatta gggtttccaa ttgaattctt
     4861 cagatccgtt atggaattat gtcaaataat ttattcaaat tggaaattat tgttagatcc
     4921 actcttaatc tgtttgatcc aagcttcaat tagggttttc acttgtttca atttcttgtt
     4981 atggattctg atttatctgt tgatgttaga tccactctta atctgtttga tccaagcgtt
     5041 aattagggtt ttcacttgtt tcaatttcgt gtgttggatt ctgatttatc tgttgttgat
     5101 gtgattacag tctgaacaat gggcagccac caccaccacc accacagcgg aatgagttca
     5161 ctgccgtata ggcagctaag aaaatcaggt ggcaccggcg gcagcggcgg atccatggtg
     5221 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5281 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5341 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5401 accaccttcg gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac
     5461 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     5521 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     5581 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5641 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5701 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5761 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5821 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5881 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaagggactg
     5941 cagcctaaga agaagagaaa ggttggagga gtcgactcga gtgcgtaaaa tcgatcgttc
     6001 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     6061 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     6121 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     6181 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     6241 agatctgagt gacctaagct agcctagtgt ctgaggcggc caacaaacta ttcgtcacgt
     6301 cgacaaaatt tagaacgaac ttaattatga tctcaaatac attgatacat atctcatcta
     6361 gatctaggtt atcattatgt aagaaagttt tgacgaatat ggcacgacaa aatggctaga
     6421 ctcgatgtaa ttggtatctc aactcaacat tatacttata ccaaacatta gttagacaaa
     6481 atttaaacaa ctatttttta tgtatgcaag agtcagcata tgtataattg attcagaatc
     6541 gttttgacga gttcggatgt agtagtagcc attatttaat gtacatacta atcgtgaata
     6601 gtgaatatga tgaaacattg tatcttattg tataaatatc cataaacaca tcatgaaaga
     6661 cactttcttt cacggtctga attaattatg atacaattct aatagaaaac gaattaaatt
     6721 acgttgaatt gtatgaaatc taattgaaca agccaaccac gacgacgact aacgttgcct
     6781 ggattgactc ggtttaagtt aaccactaaa aaaacggagc tgtcatgtaa cacgcggatc
     6841 gagcaggtca cagtcatgaa gccatcaaag caaaagaact aatccaaggg ctgagatgat
     6901 taattagttt aaaaattagt taacacgagg gaaaaggctg tctgacagcc aggtcacgtt
     6961 atctttacct gtggtcgaaa tgattcgtgt ctgtcgattt taattatttt tttgaaaggc
     7021 cgaaaataaa gttgtaagag ataaacccgc ctatataaat tcatatattt tcctctccgc
     7081 tttgaattgt ctcgttgtcc tcctcacttt catcagccgt tttgaatctc cggcgacttg
     7141 acagagaaga acaaggaaga agtctaagag agaaagtaag agataatcca ggagattcat
     7201 tctccgtttt gaatcttcct caatctcatc ttcttccgct ctttctttcc aaggtaatag
     7261 gaactttctg gatctacttt atttgctgga tctcgatctt gttttctcaa tttccttgag
     7321 atctggaatt cgtttaattt ggatctgtga acctccacta aatcttttgg ttttactaga
     7381 atcgatctaa gttgaccgat cagttagctc gattatagct accagaattt ggcttgacct
     7441 tgatggagag atccatgttc atgttacctg ggaaatgatt tgtatatgtg aattgaaatc
     7501 tgaactgttg aagttagatt gaatctgaac actgtcaatg ttagattgaa tctgaacact
     7561 gtttaaggtt agatgaagtt tgtgtataga ttcttcgaaa ctttaggatt tgtagtgtcg
     7621 tacgttgaac agaaagctat ttctgattca atcagggttt atttgactgt attgaactct
     7681 ttttgtgtgt ttgcagctca taaaaatctg ccttggactc tcaaattaac ggcgaaacac
     7741 catggatcat tatcttgata ttagacttag acctgatcct gaatttcctc ctgctcaact
     7801 tatgtctgtt ctttttggaa agcttcatca agctcttgtt gctcaaggag gagatagaat
     7861 tggagtttct tttcctgatc ttgatgaatc tagatctaga cttggagaaa gacttagaat
     7921 tcatgcttct gctgatgatc ttagagctct tcttgctaga ccttggcttg aaggacttag
     7981 agatcatctt caatttggag aacctgctgt tgttcctcat cctactcctt atagacaagt
     8041 ttctagagtt caagctaagt ctaatcctga aagacttaga agaagattga tgaggagaca
     8101 tgatctttct gaagaagaag ctagaaagag aattcctgat actgttgcta gagctcttga
     8161 tcttcctttt gttactctta gatctcaatc tactggacaa cattttagac tttttattag
     8221 acatggacct cttcaagtta ctgctgaaga aggaggattt acttgttatg gactttctaa
     8281 gggaggattt gttccttggt tttgaaaggt ttcacgtatc ttcggccatg aagaggaatc
     8341 atatgaagat gaagatgaaa tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt
     8401 gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata ttaaatgaat
     8461 gtaagatctc attataatga ataaacaaat gtttctataa tccattgtga atgttttgtt
     8521 ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat ggaatatgat
     8581 taaagataag tgagtgacct aagctagcct agtgtctctg tctgtgagac ctctggttta
     8641 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     8701 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     8761 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     8821 atagtgcagt cggcttctga cgttcagtgc agccttcttc tgaaaacgac atgtcgcaca
     8881 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     8941 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     9001 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     9061 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     9121 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     9181 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     9241 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     9301 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     9361 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     9421 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     9481 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     9541 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     9601 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     9661 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     9721 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacat
     9781 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     9841 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     9901 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     9961 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
    10021 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
    10081 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
    10141 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
    10201 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
    10261 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
    10321 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
    10381 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
    10441 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
    10501 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
    10561 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
    10621 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
    10681 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
    10741 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
    10801 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
    10861 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
    10921 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
    10981 tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
    11041 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
    11101 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
    11161 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
    11221 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
    11281 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
    11341 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
    11401 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
    11461 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
    11521 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
    11581 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
    11641 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
    11701 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
    11761 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
    11821 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
    11881 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
    11941 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
    12001 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
    12061 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
    12121 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg
    12181 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
    12241 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
    12301 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
    12361 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
    12421 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
    12481 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
    12541 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
    12601 gaaaacctct gacacatgca gctcccggag acagtcacag cttgtctgta agcggatgcc
    12661 gggagcagac aagcccgtca gggcgcgtca gcgggt
//
