LOCUS       sequence-444789-       11178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control plasmid without Cas and Csy4 for Nicotiana leaf assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Bircheneder M, Schreiber T, Tissier A, Parniske M
  TITLE     A quantitative assay for the efficiency of RNA-guided genome editing
            in plants.
  JOURNAL   Plant J. 2024 Sep;119(5):2564-2577. doi: 10.1111/tpj.16931. Epub 
            2024 Jul 20.
  PUBMED    39032106
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16931";
            journalName: "Plant J"; date: "2024-09"; volume: "119"; issue: "5"; 
            pages: "2564-2577"
COMMENT     Sequence Label: pMB24
FEATURES             Location/Qualifiers
     source          1..11178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..158
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     73..92
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(173..190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(344..932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(424..443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1002..1021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1003..1021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1026..1042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1026..1042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1144..1163)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1155..1964
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1222..1241)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2220..2244
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2371..2550
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2403..2586
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5038..5055
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    5066..5085
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     terminator      6787..7039
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7136..7160
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8461..9090
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9519..10592
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10658..10852
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11088..11110)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
       61 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
      121 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
      181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      961 ctgacgctca taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     1021 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1081 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     1141 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1201 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1261 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1321 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1381 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1441 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1501 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1561 attgtccttt taacagcgat cgcgtatttc gtcttgctca ggcgcaatca cgaatgaata
     1621 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1681 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1741 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1801 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     1861 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     1921 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     1981 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     2041 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agacccctgc cggtaacatg
     2101 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     2161 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     2221 ggcaggatat attgtggtgt aaacaaattg acgcttagac ggattacggg tctcagcggt
     2281 acggcgggat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     2341 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     2401 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     2461 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     2521 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
     2581 tctgcaccgg atcttctgga agcaccatga cgagtaaagt gtatgatcct gagcaaagga
     2641 agagaatgat cacaggtcct caatggtggg ctaggtgtaa acagatgaat gttcttgact
     2701 ctttcatcaa ctactacgat tctgagaaac atgccgaaaa cgcagtgatt ttcctccatg
     2761 gaaatgctgc ttcaagctat ctctggagac acgttgtccc acatatagaa ccagtagcta
     2821 gatgcattat accggatttg attggtatgg gcaaaagtgg gaaatcaggg aatggatcct
     2881 acaggttatt ggaccactac aagtatctca cagcttggtt tgaactactt aacttgccta
     2941 agaagatcat attcgtggga catgattggg gagcatgtct tgcctttcac tattcctatg
     3001 aacatcagga caaaataaag gccattgtcc atgcagagtc tgttgttgac gtgattgaaa
     3061 gctgggatga atggcctgac attgaggagg atattgcgtt aatcaagagt gaagaaggcg
     3121 agaaaatggt tcttgaaaac aatttctttg tcgaaactat gttgccgtct aaaatcatga
     3181 gaaaactgga accagaagaa ttcgcagcat acttagaacc ctttaaggag aaaggagaag
     3241 tcagacgacc aactctgtct tggcctagag agattccatt ggttaagggt ggcaaaccag
     3301 atgttgtaca aattgtgcga aattataacg cttatctgcg tgcttcagat gatcttccta
     3361 agatgtttat tgagtcagat cccggtttct ttagcaatgc gattgtagag ggtgctaaga
     3421 aatttccaaa taccgaattc gtgaaggtta aaggactaca cttttctcaa gaagatgcac
     3481 ctgatgagat ggggaagtac ataaagtcct ttgtagagcg tgttcttaag aatgagcagt
     3541 aaaagggcta agacctaagc tagcctagaa tccggccatg ctagagtccg caaaaatcac
     3601 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
     3661 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     3721 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     3781 accaaaatcc agtgaccttg agtgacctaa gctagcctag tgtcgggcta gataattagg
     3841 ctaaactatc cttaaggatt gtagataatt aggctaaact atccttaagg tgtcgcgggg
     3901 agagaggatt ttgaggaaat aattaattga attacttgta ttattgataa agtaatttag
     3961 ataagttgtt agtacaactt attgcaacta tggatagaca aaaatcactt attttaaggg
     4021 gagaagtata taacaactta ttataaaatt tctgagcagc cggcctcctc caatcaatca
     4081 tagagagtgg agcccattct ggaaaaccaa ggaaccccca acttgcactg gtgcggtggc
     4141 ccaattcaaa aaaaacgggg cccagcaaag taacgccgtt gtaacgattt atcaatccaa
     4201 actcaaaagg cggcgagatg cgtttaactc cgtgaaatta acaaaccgcc aacaacttgc
     4261 aatttgcaac taccgtttcc aagaagaact caaccacaca acgtatccta tcccaaacca
     4321 cacgcaacta gtgacgcgtc ataaggacac gtgtcacaat ttgactggtt aataatttca
     4381 ccgcttttgc tataaattac ctccaatccc cttagcttct tcacaattca gttcccaacc
     4441 ctaacaattc ttgttcatat cgcttctctc tactttcaag gtatgatcca atttctctct
     4501 tctttctctg taatcctttc gttgagtttt gtttccgatc aatcataggt agttttcttg
     4561 tttcgaagca tgagatctag gaattttttg tgattttcca aaattgagat cggtttgaaa
     4621 ttgaatttta cagcttgaat ctcagatctt gttttatcaa tgttttcgat ggcttgcgat
     4681 gtagatctat gataattgtg gttcagtttt gttaggaatc gatttcggtt tagcaattgc
     4741 agattaatta gggtttccaa ttgaattctt cagatccgtt atggaattat gtcaaataat
     4801 ttattcaaat tggaaattat tgttagatcc actcttaatc tgtttgatcc aagcttcaat
     4861 tagggttttc acttgtttca atttcttgtt atggattctg atttatctgt tgatgttaga
     4921 tccactctta atctgtttga tccaagcgtt aattagggtt ttcacttgtt tcaatttcgt
     4981 gtgttggatt ctgatttatc tgttgttgat gtgattacag tctgaacaat gggcagccac
     5041 caccaccacc accacagcgg aatgagttca ctgccgtata ggcagctaag aaaatcaggt
     5101 ggcaccgaag atgcaaagaa cattaagaaa gggcctgcac cattctatcc acttgaggac
     5161 ggtacagccg gtgagcaatt gcataaggcc atgaagagat atgcacttgt accagggacg
     5221 atagccttta ctgatgctca cattgaggtc aatatcactt atgcggaata ttttgaaatg
     5281 tcagtcagat tggcagaagc tatgaaaagg tatggcttaa ataccaatca cagaattgtg
     5341 gtgtgttctg agaatagttt acagttcttc atgccagtgt tgggtgcact atttattggt
     5401 gtagctgttg ctccagctaa cgacatttac aatgagagag aactactcaa ctctatgaat
     5461 atttcacagc ccacagtcgt atttgtgagc aagaaaggat tgcagaagat acttaacgtt
     5521 cagaagaaac ttccgattat acagaagatc attattatgg acagcaaaac tgactaccaa
     5581 ggctttcagt ctatgtatac atttgtgaca tctcatttac ctccaggctt taatgagtat
     5641 gacttcgttc cagaatcatt tgatcgtgat aagactatcg ctcttattat gaatagctct
     5701 ggatccactg gtctgccgaa gggagtagca ttgcctcata ggacagcttg tgttaggttt
     5761 tcacacgcaa gagatccgat atttggaaat cagatcattc ctgatacggc tatattgagt
     5821 gttgtaccat ttcaccatgg ctttggaatg tttaccacac ttgggtactt gatttgcggc
     5881 tttagagtgg tactgatgta caggttcgag gaggaacttt tcctacgaag tctccaagat
     5941 tacaaaatcc aatccgcttt gttagttcct acactgtttt ccttcttcgc gaaatctacc
     6001 ctgattgaca aatatgatct ctctaatctc cacgaaatag caagtggtgg tgcacctctt
     6061 tcaaaagagg ttggagaagc agttgccaaa cgttttcatc taccaggtat cagacaagga
     6121 tatggtctta ctgagacaac atcagccata ttaattaccc ctgaaggcga tgataagcct
     6181 ggtgctgttg gaaaagttgt tccattcttc gaagcaaaag tggtagactt ggatactggc
     6241 aagacgcttg gagtcaacca aagaggtgaa ctgtgcgtta gagggcctat gatcatgtct
     6301 gggtacgtta acaatcctga ggcaaccaat gcgttaattg acaaagatgg ctggttacat
     6361 agtggagata ttgcgtactg ggacgaagat gagcacttct tcatagtgga taggttgaag
     6421 tctctcatca agtacaaagg ttatcaagta gctcctgctg aattagagtc aatccttctt
     6481 caacatccca acattttcga tgctggagtg gccggattgc ccgatgatga tgccggtgaa
     6541 ttgcccgcag ctgttgttgt gctggaacat ggtaagacta tgacagagaa ggaaatagtg
     6601 gactatgtcg ctagccaagt tactactgct aagaaactcc gtggaggggt tgtctttgtt
     6661 gatgaagtcc caaaaggatt gactgggaaa cttgatgctc gaaagataag agagattctc
     6721 attaaagcta agaagggtgg aaaatccaaa ctataaaagg gctaagacct aagctagcct
     6781 agaatcgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     6841 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     6901 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     6961 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     7021 tcatctatgt tactagatct gagtgaccta agctagccta gtgtctgagt agataattag
     7081 gctaaactat ccttaaggtc tgtctgtgag acctctggtt taaactatca gtgtttgaca
     7141 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     7201 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     7261 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     7321 gacgttcagt gcagccttct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     7381 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     7441 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     7501 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     7561 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     7621 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     7681 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     7741 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     7801 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     7861 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     7921 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     7981 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     8041 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     8101 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     8161 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     8221 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac atctcaaccg tgcggctgca
     8281 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     8341 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     8401 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     8461 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     8521 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     8581 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     8641 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     8701 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     8761 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     8821 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     8881 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     8941 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     9001 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     9061 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     9121 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     9181 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     9241 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     9301 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     9361 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     9421 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     9481 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     9541 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     9601 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     9661 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     9721 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     9781 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     9841 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     9901 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     9961 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
    10021 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
    10081 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
    10141 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
    10201 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
    10261 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
    10321 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
    10381 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
    10441 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
    10501 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
    10561 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
    10621 ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg tacattggga
    10681 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
    10741 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
    10801 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
    10861 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
    10921 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
    10981 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
    11041 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    11101 cagctcccgg agacagtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    11161 cagggcgcgt cagcgggt
//
