LOCUS       sequence-444820-        9553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mGSDME D270A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Zhang Z, Zhang Y, Xia S, Kong Q, Li S, Liu X, Junqueira C, Meza-Sosa
            KF, Mok TMY, Ansara J, Sengupta S, Yao Y, Wu H, Lieberman J
  TITLE     Gasdermin E suppresses tumour growth by activating anti-tumour 
            immunity.
  JOURNAL   Nature. 2020 Mar;579(7799):415-420. doi: 10.1038/s41586-020-2071-9. 
            Epub 2020 Mar 11.
  PUBMED    32188940
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2071-9"; journalName: "Nature"; date: "2020-03";
            volume: "579"; issue: "7799"; pages: "415-420"
COMMENT     Sequence Label: pLVX-puro-mGSDME D270A
FEATURES             Location/Qualifiers
     source          1..9553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4388..4887
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4423..4446)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4820..4839
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4908..5507
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4908..4927)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5404..5424
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5521..6109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5574..5594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5992..6003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6316..6949
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7078..7094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7078..7094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7091..7113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7102..7118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7126..7132,7133..7150,7151..7156))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7171..7192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7309..7326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7480..8068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7560..7579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8239..9030,9031..9099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8862..8881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9100..9204)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9252..9386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9289..9308)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9343..9362
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 atgtttgcca aagcaactcg aaattttctt aaagaagttg atgctggagg agacctgatt
     2881 tcagtctcac acttgaacga ctctgacaag ctgcaacttc taagtctggt gaccaaaaag
     2941 aagagatact ggtgctggca gagacccaag taccagattt tatctgccac cctggaagat
     3001 gtactcacag aagggcactg tctcagtcca gtggttgtgg agtcagactt cgtgaaatac
     3061 gagagcaagt gtgagaacca taagagcggg gctattggga cagtcgtggg gaaggtcaag
     3121 ctgaacgttg gtggcaaagg cgtggtggag agtcactctt cgtttggaac cctgaggaag
     3181 caggaggtgg acgtgcagca gctcatccag gatgccgtca agagaacagt taatatggac
     3241 aacctggtac ttcagcaggt gctagagagc aggaacgagg tcctgtgtgt gctgacgcag
     3301 aagatcatga ccacgcagaa gtgcgtgatt tctgagcatg tgcagtcgga ggagacgtgc
     3361 ggaggcatgg tggggatcca gaccaagact atacaggtgt cagcaacgga ggatgggact
     3421 gtcaccacag acaccaatgt agtgctggag atccctgctg ccaccaccat tgcctatggc
     3481 atcatggagc tgtttgtgaa acaagatggc cagtttgaat tctgcctcct ccaagggaaa
     3541 catggtggct tcgagcatga gaggaaacta gactctgtct acttggaccc cctggcctac
     3601 agagagttcg cctttctgga catgctggct gggggtcaag ggatctcttc gcaggacggg
     3661 cccctacgag ttgtaaaaca agcaaccctg cacctagaga ggagtttcca tccctttgcg
     3721 gtgttacctg cccagcagca gagggcgctg ttctgtgtcc tgcagaaaat cctgtttgat
     3781 gaagaactcc ttcgggccct ggagcaagtg tgtgatgatg tggctggtgg cctctggtcc
     3841 tcacaggctg tattggcaat ggaggagctg accgacagtc agcagcagga cctcacagcc
     3901 ttcttgcagc tggtgggata caggatacaa ggagagcatc ctggcccaca ggatgaggtc
     3961 agcaaccaga agctctttgc aacagcctac ttcctggtca gcgcactagc agaaatgcct
     4021 gataatgcca cagttttcct ggggacttgc tgcaaactcc atgttatttc ttcgctgtgc
     4081 tgcttgctcc atgctctgtc tgatgacagc gtgtgtgatt ttcacaaccc caccttggct
     4141 cctctgagag acacagagag gtttggcatc gtgcagcgat tgtttgcctc tgctgacatt
     4201 gccctggaga ggatgcagtt ttctgcgaaa gccaccatcc tgaaggactc ttgcatcttc
     4261 ccgctaattc ttcacatcac tctaagtggg ctcagcactc taagcaaaga gcatgaggaa
     4321 gagctttgtc agtcaggaca tgctacaggt caagactagg gatcccgcga ctctagataa
     4381 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     4441 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     4501 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     4561 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     4621 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     4681 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     4741 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     4801 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     4861 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
     4921 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     4981 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     5041 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5101 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5161 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5221 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5281 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5341 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5401 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5461 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac
     5521 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5581 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5641 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5701 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5761 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5821 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5881 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     5941 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6001 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6061 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
     6121 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     6181 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     6241 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6301 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6361 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     6421 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     6481 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     6541 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     6601 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     6661 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     6721 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     6781 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6841 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6901 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6961 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7021 ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7201 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7921 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9241 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9301 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9361 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     9421 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     9481 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     9541 caaacttagt agt
//
