LOCUS       sequence-444835-        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible lentiviral vector for 2xHA GPR153 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, 
            Iglesias-Bartolome R
  TITLE     GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates 
            Epithelial Proliferation, Migration, and Cellular Respiration.
  JOURNAL   J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 
            10.1016/j.jid.2023.12.002. Epub 2023 Dec 14.
  PUBMED    38103827
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.12.002"; journalName: "J Invest Dermatol"; date:
            "2024-06"; volume: "144"; issue: "6"; pages: "1311-1321.e7"
COMMENT     Sequence Label: pInducer20 2xHA GPR153
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             34..60
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgtacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca
       61 ggatccggag atccacctgt cgcaaccatg tcagacgaaa ggagacttcc tggaagtgcg
      121 gtcgggtggc ttgtatgcgg tggtctcagt ttgctggcca atgcttgggg tatactgtct
      181 gtaggagcga agcagaagaa atggaagccc cttgaatttc tgctttgcac attggcagct
      241 acccacatgc tcaacgttgc ggtgccgatt gcgacttact ccgtggtgca acttcggcgc
      301 caaaggccag acttcgaatg gaacgaaggc ctgtgcaagg ttttcgtaag tactttctat
      361 acattgactc ttgctacatg ctttagcgta acatctctga gttatcatcg gatgtggatg
      421 gtctgttggc cggtcaacta ccgcctgtcc aacgctaaaa aacaggcggt acatacggta
      481 atggggatat ggatggtttc cttcatactc tccgcactcc cagccgttgg atggcacgat
      541 acgtcagaac gattttacac tcacgggtgt cgatttatcg tcgccgaaat aggcctcggt
      601 ttcggtgtat gttttttgct tctggttggg gggagcgtcg ccatgggggt aatctgcaca
      661 gcgatcgcac ttttccaaac acttgcagtc caagtcggac gccaagccga tagacgagcc
      721 ttcactgttc ccacaatagt cgtggaagat gcgcaaggga aacgaagatc cagcatcgac
      781 ggcagcgaac ctgcgaagac gtctctccaa acgacgggcc tcgtgacaac aattgttttt
      841 atctacgact gtctcatggg gttcccggtt ttggttgtgt cattcagcag cctcagggcg
      901 gatgcgtcag cgccgtggat ggccctttgt gttctttggt gcagcgttgc gcaagcgttg
      961 ttgctcccag tattcctgtg ggcttgcgat aggtacagag cagatctgaa agctgtgaga
     1021 gagaagtgca tggcgttgat ggcaaatgac gaggagtccg atgacgaaac cagtcttgaa
     1081 ggaggcataa gccctgatct tgtattggag cgatcactcg attacggata tggaggtgac
     1141 ttcgtggcgc ttgatcgcat ggcaaagtac gagatatctg cacttgaggg gggacttcct
     1201 caactgtacc cactccggcc cttgcaggaa gataaaatgc agtacctgca ggttcctcca
     1261 acgaggcggt tcagtcacga cgatgcggat gtatgggctg cagtccccct tcctgcattc
     1321 ttgcccagat gggggtccgg ggaggacttg gccgcactgg ctcacttggt tcttccagcc
     1381 ggacctgaac ggagacgagc ttctcttctc gcctttgctg aggatgcgcc cccgtcacgc
     1441 gcacgacgga gatcagccga aagcctgctt tccctgcgac ctagtgctct cgattctggg
     1501 cccagaggag cccgggactc cccgcctggg tctccgagac gccggcctgg tcctgggccg
     1561 cggagcgcaa gcgcgagcct tcttcctgat gctttcgccc ttactgcatt tgagtgtgag
     1621 ccgcaagcat tgaggcggcc gcccggtcca tttcccgccg cacctgcggc acctgacggg
     1681 gcagatccag gtgaggcccc aacccccccg tcatctgcgc agagaagtcc tggtccgcgg
     1741 ccctcagcgc atagccacgc tgggtcactt cgacctggac tgtcagccag ttggggagag
     1801 ccaggtggcc ttcgagccgc cgggggagga gggagtactt cttctttttt gagtagtccg
     1861 agtgagtctt ccggctacgc cactttgcat agtgactctc ttggttccgc aagttag
//
