LOCUS       sequence-444902-        1251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for 2xHA HCAR3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, 
            Iglesias-Bartolome R
  TITLE     GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates 
            Epithelial Proliferation, Migration, and Cellular Respiration.
  JOURNAL   J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 
            10.1016/j.jid.2023.12.002. Epub 2023 Dec 14.
  PUBMED    38103827
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.12.002"; journalName: "J Invest Dermatol"; date:
            "2024-06"; volume: "144"; issue: "6"; pages: "1311-1321.e7"
COMMENT     Sequence Label: pENTR1A 2xHA HCAR3
FEATURES             Location/Qualifiers
     source          1..1251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             34..60
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgtacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca
       61 ggatccggag atccacctgt cgcaaccatg aaccggcacc acctccagga ccacttcctg
      121 gagattgaca aaaagaactg ctgtgtgttc agggacgatt tcatcgccaa ggtactcccc
      181 cctgtgcttg ggttggagtt catcttcggg ctgcttggca atgggcttgc attgtggatt
      241 ttttgcttcc atcttaagtc atggaagtcc agtcggattt tcttgttcaa tttggccgta
      301 gcggatttcc tcctcattat ctgccttccc tttgtaatgg attactacgt gagacgctct
      361 gattggaaat tcggggatat cccgtgccgg ctggtactct tcatgtttgc gatgaacagg
      421 caagggagca tcattttctt gactgtggtc gccgttgatc gctatttcag agtggttcat
      481 cctcaccacg ctcttaataa gatatccaac tggacagcag ctattatctc atgtttgttg
      541 tgggggataa cggtcgggct gactgtccat cttttgaaga aaaagttgtt gatacagaat
      601 gggacggcta atgtatgtat atccttctct atttgccata cgtttcgctg gcacgaagct
      661 atgtttctct tggaattttt cctgcctctg ggtataatct tgttctgctc cgccagaata
      721 atttggtcat tgagacagag acagatggac aggcacgcga agattaaacg ggcaatcacg
      781 ttcataatgg tggtagcaat cgtatttgtc atctgcttcc tgccgtcagt ggttgtcagg
      841 attcacatct tctggctcct gcacaccagc gggactcaga actgcgaagt atatcgatca
      901 gtggacctgg cattcttcat cacgctcagt tttacgtaca tgaacagcat gcttgaccct
      961 gtcgtgtatt atttttcaag cccctcattc ccgaactttt ttagcacact gattaatcgc
     1021 tgcctccaaa ggaagataac aggcgaaccc gataataacc ggtccacctc tgtggaactc
     1081 acgggagacc ccaacaagac caggggtgcc cccgaggcgc tcattgcgaa ctctggggaa
     1141 ccctggtccc ctagctacct tggaccaacg tcaaataatc atagtaaaaa ggggcactgt
     1201 catcaggagc cagccagtct tgaaaagcag cttggctgtt gtatcgaata a
//
