LOCUS       sequence-444935-        7304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of Zaphod transposase 
            (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-Zaphod
FEATURES             Location/Qualifiers
     source          1..7304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3492..3510)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3493..3510)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3530..3547)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3536..3760
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3806..4234
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3893..3912)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4103..4124
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4243..4263)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4248..4577
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4428..4563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4490..4509
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    4743..4864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4780..4799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4834..4853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4913..4929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4913..4929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4926..4948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4937..4953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4961..4967,4968..4985,4986..4991))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5006..5027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5144..5161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5315..5903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5395..5414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6074..6865,6866..6934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6697..6716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6935..7039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7114..7133)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg ccgccaccat
      721 gagcaccagc cgtgatcgtg atcgcaaccg taaatacgaa agcggcgcgc agaaacgtaa
      781 gaaagcggag aaaaagaagg aaatcgaaaa cctgcagcgc ggcggcttac tgaaatttct
      841 gaagaccggc aacaacgaaa gcgaacagac cagcgtggaa catgacccgc ataaaatccg
      901 cgagcaggat attaccaaca gcctgttcaa cgaggatctg attaccgacc agaacaacga
      961 gaacgaacag gagaactaca gccaccagat tcataacctg aaccgcagcg atgaaattcc
     1021 ggcgaccagc tattcattac tgaacgagga cctgattacc gatcagaaca acgaaaacga
     1081 gaaggaaaac tatagctacc agatccataa cagcgaagcg accaatgcga ccttagcgtg
     1141 ccctctggaa gacgaagaaa actacatcca cgaaatcggc aaactggatg aaaaagacga
     1201 aaccagcggt gcgctgctgt gcttactgga tgatgatccg ggcaaatggc ctagccatcc
     1261 tagcgatagc gtgcgcatgt ttctggcgaa acacaacctg attcagatca acgattacga
     1321 ctttccgctg gatgagcatg gccgcaaatt tagcattagc ttctacacca agattctgag
     1381 caacggcgag cgcaattgtc gtaactggct gctgtacagc aaatttaagg acaaggtgtt
     1441 ctgctactac tgctacatgt tccataacca gagcagccgt agcgcgttag cgaatgaagg
     1501 catctgcgat tggaaacatc tgggcagccg cctgaaggaa catgaaacca gcctggcgca
     1561 caaaaaggcg catatgcagc tgctggagct gaaaaagcgc ctgaacaagc agcagaccat
     1621 cgacagcatt cagcagcgcc aagtgaacgc cgacattgaa cattggcgcg atgtgctgaa
     1681 acgcattatc ctgtgcgtgc agtatctggc caaacataac gatgcgtttc gcggcaccaa
     1741 cagcaccatt tttaccgaga acaacggcaa atttctgggc ctggtggaaa tgatcagcaa
     1801 atttgatgcg cgcatggcgg aacatgtgcg ccgcattaag aacaacgaaa ccaacgatca
     1861 ttacctgggc tggcgcatcc agaacgaaat tattaacctg atgggcaaca aagtgcgcga
     1921 agagatcatt cacaacaacc tgaagagcaa atactatagc gtgcagctgg attgcacccg
     1981 cgacaaaagc catgtggagc agctgacctt tattatccgc attgtggaac tgccggagga
     2041 ggagaacaaa gaagtggaga tcaacgaata cttcattggc tttaccccgg tggttaagag
     2101 caccggcttt gacttaagcg aggagctgaa aaaacagctg agcattctgg gcattgatct
     2161 gaaagattgc cgcggccagg catatgataa tggcgccaac atggtgggca aagaaaaagg
     2221 cgtgcaggca cgcatcttag ccgagaatcc tgaggcgttc ttcgttcctt gcaccgcgca
     2281 tagcctgaac ttactgctgg gtgacgtggc aagcaccgtt cctatagcga tgaccttctt
     2341 tggcaccatc cagcgcctgt ataccatttt tagcggcagc gcccagcgtt ggaacattct
     2401 gatgaaacac atcagcaacc tgaccctgca gcctttaagc gatacccgtt gggaatgccg
     2461 tctgaatgcc gtgaaggcca ttcgctttaa cctggataaa atccgcgatg cgctggagga
     2521 actgagcgaa accaccgaag atccgcagat taaaagcgag agctggagcc tggcggaaaa
     2581 cgaaattaac ttcgagttcg cgctgagcac cgtgatttgg tacgagctgc tgctggcgat
     2641 caacaacgtg agcaaaagcc tgcagaaaaa caaccgcagc aagtttggct actttacctt
     2701 taagcgcatc aagaacatct tcctgaactt tcgcgagaac ggcttcagca aaagcaaaga
     2761 aaccgcctgc gagatctgca acaaaagcga tatcccgatc aagtacaaag aaatccgcac
     2821 ccgcaaacgc aaacgcctgc atgattatga aggcgaagat agccgcacca acaaccctga
     2881 aaccaacttc tatcgcgatt atttcctggc gattatcgat cagggcattg tgagcattga
     2941 agaacgcttt gaacagattg agcagcatac cgcgatcttt ggcttcctgt acaacattcc
     3001 tgccctgaaa aacctgaagg aagaggagct gaagaaatat tgcatggatc tggatatcgc
     3061 gttacgcgat ggcgagaacc gcgatattag cgatagcgac ctgtatgacg agattaagat
     3121 cttctgcaac atcttctgcg ataacgataa cctgagctat gcgacccctc tgcaatgcct
     3181 gaacatgatc tgcaaaatcc atggcagctt tcctaacctg agcattgccc tgcgcattct
     3241 gttaaccatt ccggtggcaa ccgcatcagc ggaacgtagc ttcagcaaac tgaaactgat
     3301 caagaactat ctgcgcacca ccatgaccca ggaacgctta agcaacctgg cgctgctgag
     3361 catcgaacat aaactgtgcg aaaacctgga ttacaacaac atcattagcg acttcgccga
     3421 aatgaaagcc cgcaagatta acttcatgga gtaatgagcg gccgctcgag catgcatcta
     3481 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
     3541 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3601 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3661 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3721 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     3781 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4021 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4141 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4201 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4261 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4321 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4381 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4441 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4501 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4561 gcctaggctt ttgcaaaaag ctccccgaaa tgaccgacca agcgacgccc aacctgccat
     4621 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     4681 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     4741 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4861 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     4921 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4981 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5041 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5101 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     5161 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5221 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5281 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5341 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5401 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5461 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5521 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5581 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5641 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5701 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5761 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5821 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5881 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5941 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6001 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6061 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6121 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6181 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6241 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6301 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6361 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6421 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6481 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6541 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6601 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6661 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6721 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6781 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6841 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6901 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6961 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7021 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     7081 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7141 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     7201 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     7261 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
