LOCUS       sequence-444939-        9636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P
  TITLE     Improving the yeast two-hybrid system with permutated fusions 
            proteins: the Varicella Zoster Virus interactome.
  JOURNAL   Proteome Sci. 2010 Feb 15;8:8. doi: 10.1186/1477-5956-8-8.
  PUBMED    20205919
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteome 
            Sci."; date: "2010-02-15"; volume: "8:8. doi"; pages: " 
            10.1186/1477-5956-8-8"
COMMENT     Sequence Label: pGADCg
FEATURES             Location/Qualifiers
     source          1..9636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1560..1684
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1709..1714,1715..1732,1733..1739)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1793..2452
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1859..1878)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2794..3099
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2978..2997
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3140..3264)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3278..3619
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     terminator      4064..4251
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4119..4143)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4368..5462)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5463..5868)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5910..5926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5910..5926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5923..5945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5934..5950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5958..5964,5965..5982,5983..5988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6003..6024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6079..6112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6190..6223
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6292..6309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6463..7051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6543..6562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7222..8013,8014..8082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7845..7864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8083..8187)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8255..8273
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8311..8333)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8433..8452
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8469..9633
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgcatcaa
     1561 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     1621 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     1681 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     1741 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     1801 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1861 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1921 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1981 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     2041 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     2101 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     2161 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     2221 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     2281 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     2341 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     2401 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg
     2461 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     2521 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     2581 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     2641 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     2701 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     2761 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     2821 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     2881 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     2941 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     3001 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     3061 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     3121 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     3181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     3241 ttcagctttc ttgtacaaag tggttgatgg taccgccgcc aattttaatc aaagtgggaa
     3301 tattgctgat agctcattgt ccttcacttt cactaacagt agcaacggtc cgaacctcat
     3361 aacaactcaa acaaattctc aagcgctttc acaaccaatt gcctcctcta acgttcatga
     3421 taacttcatg aataatgaaa tcacggctag taaaattgat gatggtaata attcaaaacc
     3481 actgtcacct ggttggacgg accaaactgc gtataacgcg tttggaatca ctacagggat
     3541 gtttaatacc actacaatgg atgatgtata taactatcta ttcgatgatg aagatacccc
     3601 accaaaccca aaaaaagaga tcctcgagta cgacgtacca gattacgctt agtgatcacg
     3661 atacgggatc catcgagctc gagctgcaga tgaatcgtag atactgaaaa accccgcaag
     3721 ttcacttcaa ctgtgcatcg tgcaccatct caatttcttt catttataca tcgttttgcc
     3781 ttcttttatg taactatact cctctaagtt tcaatcttgg ccatgtaacc tctgatctat
     3841 agaatttttt aaatgactag aattaatgcc catctttttt ttggacctaa attcttcatg
     3901 aaaatatatt acgagggctt attcagaagc tttggacttc ttcgccagag gtttggtcaa
     3961 gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa
     4021 gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt
     4081 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     4141 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
     4201 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gccggtcgaa
     4261 attcccctac cctatgaaca tattccattt tgtaatttcg tgtcgtttct attatgaatt
     4321 tcatttataa agtttatgta caaatatcat aaaaaaagag aatcttttta agcaaggatt
     4381 ttcttaactt cttcggcgac agcatcaccg acttcggtgg tactgttgga accacctaaa
     4441 tcaccagttc tgatacctgc atccaaaacc tttttaactg catcttcaat ggccttacct
     4501 tcttcaggca agttcaatga caatttcaac atcattgcag cagacaagat agtggcgata
     4561 gggttgacct tattctttgg caaatctgga gcagaaccgt ggcatggttc gtacaaacca
     4621 aatgcggtgt tcttgtctgg caaagaggcc aaggacgcag atggcaacaa acccaaggaa
     4681 cctgggataa cggaggcttc atcggagatg atatcaccaa acatgttgct ggtgattata
     4741 ataccattta ggtgggttgg gttcttaact aggatcatgg cggcagaatc aatcaattga
     4801 tgttgaacct tcaatgtagg aaattcgttc ttgatggttt cctccacagt ttttctccat
     4861 aatcttgaag aggccaaaac attagcttta tccaaggacc aaataggcaa tggtggctca
     4921 tgttgtaggg ccatgaaagc ggccattctt gtgattcttt gcacttctgg aacggtgtat
     4981 tgttcactat cccaagcgac accatcacca tcgtcttcct ttctcttacc aaagtaaata
     5041 cctcccacta attctctgac aacaacgaag tcagtacctt tagcaaattg tggcttgatt
     5101 ggagataagt ctaaaagaga gtcggatgca aagttacatg gtcttaagtt ggcgtacaat
     5161 tgaagttctt tacggatttt tagtaaacct tgttcaggtc taacactacc tgtaccccat
     5221 ttaggaccac ccacagcacc taacaaaacg gcatcagcct tcttggaggc ttccagcgcc
     5281 tcatctggaa gtgggacacc tgtagcttcg atagcagcac caccaattaa atgattttcg
     5341 aaatcgaact tgacattgga acgaacatca gaaatagctt taagaacctt aatggcttcg
     5401 gctgtgattt cttgaccaac gtggtcacct ggcaaaacga cgatcttctt aggggcagac
     5461 attagaatgg tatatccttg aaatatatat atatattgct gaaatgtaaa aggtaagaaa
     5521 agttagaaag taagacgatt gctaaccacc tattggaaaa aacaataggt ccttaaataa
     5581 tattgtcaac ttcaagtatt gtgatgcaag catttagtca tgaacgcttc tctattctat
     5641 atgaaaagcc ggttccggcg ctctcacctt tcctttttct cccaattttt cagttgaaaa
     5701 aggtatatgc gtcaggcgac ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat
     5761 tctgtgcgat agcgcccctg tgtgttctcg ttatgttgag gaaaaaaata atggttgcta
     5821 agagattcga actcttgcat cttacgatac ctgagtattc ccacagttgg gggatctcga
     5881 ctctagctag aggatcaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     5941 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6001 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6061 aacctgtcgt gccagctgat aacttcgtat aatgtatgct atacgaagtt attaggtctg
     6121 aagaggagtt tacgtccagc caagctagct tggctgcagg tcgagcggcc gcgatccgga
     6181 acccttaata taacttcgta taatgtatgc tatacgaagt tatcagctgc attaatgaat
     6241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6901 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7081 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7501 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7561 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7621 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7681 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7741 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7801 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7861 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7921 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7981 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8041 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8101 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8161 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8221 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8281 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     8341 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     8401 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     8461 gcaccataac gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata
     8521 tacaggcaac acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc
     8581 gttgcatttt cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc
     8641 tattctctag ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg
     8701 aagacgcact ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc
     8761 gaataccgct tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat
     8821 atccctatat aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct
     8881 ctacattttt tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac
     8941 tattcataga gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat
     9001 agagacaaaa tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta
     9061 tcactttctg ttcacaaagt atgcgcaatc cacatcggta tagaatataa tcggggatgc
     9121 ctttatcttg aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc gggaagtgga
     9181 gtcaggcttt ttttatggaa gagaaaatag acaccaaagt agccttcttc taaccttaac
     9241 ggacctacag tgcaaaaagt tatcaagaga ctgcattata gagcgcacaa aggagaaaaa
     9301 aagtaatcta agatgctttg ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca
     9361 aaaaagaagt atagattctt tgttggtaaa atagcgctct cgcgttgcat ttctgttctg
     9421 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt
     9481 ttacaaaaat gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg
     9541 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt
     9601 ttgttctaca aaatgaagca cagatgcttc gttgct
//
