LOCUS       sequence-444972-        3171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector, pWVO1 origin of replication (from L. lactis sbsp. 
            cremoris Wg2). Replicates in both Gram-Positive and Gram-Negative 
            bacteria. Chloramphenicol- and Erythromycin resistance marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Kok J, van der Vossen JM, Venema G
  TITLE     Construction of plasmid cloning vectors for lactic streptococci 
            which also replicate in Bacillus subtilis and Escherichia coli.
  JOURNAL   Appl Environ Microbiol. 1984 Oct;48(4):726-31. doi: 
            10.1128/aem.48.4.726-731.1984.
  PUBMED    6095756
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/aem.48.4.726-731"; journalName: "Appl Environ Microbiol"; 
            date: "1984-10"; volume: "48"; issue: "4"; pages: "726-31"
COMMENT     Sequence Label: pGK12
FEATURES             Location/Qualifiers
     source          1..3171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1292..1303
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attgtataaa gtggctctaa cttatcccaa taacctaact ctccgtcgct attgtaacca
       61 gttctaaaag ctgtatttga gtttatcacc cttgtcacta agaaaataaa tgcagggtaa
      121 aatttatatc cttcttgttt tatgtttcgg tataaaacac taatatcaat ttctgtggtt
      181 atactaaaag tcgtttgttg gttcaaataa tgattaaata tctcttttct cttccaattg
      241 tctaaatcaa ttttattaaa gttcatttga tatgcctcct aaatttttat ctaaagtgaa
      301 tttaggaggc ttacttgtct gctttcttca ttagaatcaa tcctttttta aaagtcaata
      361 ttactgtaac ataaatatat attttaaaaa tatcccactt tatccaattt tcgcttaatc
      421 ttgttcctaa cgctttctat tgttacagga tttcgtgcaa tatatataac gtgatagtgt
      481 ggttttttat agtgctttcc atttcgtata acatcactac tattccatgt atctttatct
      541 tttttttcgt ccatatcgtg taaaggactg acagccatag atacgcccaa actctctaat
      601 ttttctttcc aatcattagg aattgagtca ggatataata aaaatccaaa atttctagct
      661 ttagtatttt taatagccat gatataatta ccttatcaaa aacaagtagc gaaaactcgt
      721 atccttctaa aaacgcgagc tttcgcttat tttttttgtt ctgattcctt tcttgcatat
      781 tcttctatag ctaacgccgc aaccgcagat tttgaaaaac ctttttgttt cgccatatct
      841 gttaattttt tatcttgctc ttttgtcaga gaaatcataa ctcttttttt cgattctgaa
      901 atcaccattt aaaaaactcc aatcaaataa ttttataaag ttagtgtatc actttgtaat
      961 cataaaaaca acaataaagc tacttaaata tagatttata aaaaacgttg gcgaaaacgt
     1021 tggcgattcg ttggcgattg aaaaacccct taaacccttg agccagttgg gatagagcgt
     1081 ttttggcaca aaaattggca ctcggcactt aatggggggt cgtagtacgg aagcaaaatt
     1141 cgcttccttt ccccccattt ttttccaaat tccaaatttt tttcaaaaat tttccagcgc
     1201 taccgctcgg caaaattgca agcaattttt aaaatcaaac ccatgaggga atttcattcc
     1261 ctcatactcc cttgagcctc ctccaaccga aatagaaggg cgctgcgctt attatttcat
     1321 tcagtcatcg gctttcataa tctaacagac aacatcttcg ctgcaaagcc acgctacgct
     1381 caagggcttt tacgctacga taacgcctgt tttaacgatt atgccgataa ctaaacgaaa
     1441 taaacgctaa aacgtctcag aaacgatttt gagacgtttt aataaaaaat cggataataa
     1501 atatatataa acgtatatag atttcataaa gtctaacaca ctagacttat ttacttcgta
     1561 attaagtcgt taaaccgtgt gctctacgac caaaactata aaacctttaa gaactttctt
     1621 tttttacaag aaaaaagaaa ttagataaat ctctcatatc ttttattcaa taatcgcatc
     1681 cgattgcagt ataaatttaa cgatcactca tcatgttcat atttatcaga gctcgtgcta
     1741 taattatact aattttataa ggaggaaaaa atatgggcat ttttagtatt tttgtaatca
     1801 gcacagttca ttatcaacca aacaaaaaat aagtggttat aatgaatcgt taataagcaa
     1861 aattcatata accaaattaa agagggttat aatgaacgag aaaaatataa aacacagtca
     1921 aaactttatt acttcaaaac ataatataga taaaataatg acaaatataa gattaaatga
     1981 acatgataat atctttgaaa tcggctcagg aaaaggccat tttacccttg aattagtaaa
     2041 gaggtgtaat ttcgtaactg ccattgaaat agaccataaa ttatgcaaaa ctacagaaaa
     2101 taaacttgtt gatcacgata atttccaagt tttaaacaag gatatattgc agtttaaatt
     2161 tcctaaaaac caatcctata aaatatatgg taatatacct tataacataa gtacggatat
     2221 aatacgcaaa attgtttttg atagtatagc taatgagatt tatttaatcg tggaatacgg
     2281 gtttgctaaa agattattaa atacaaaacg ctcattggca ttacttttaa tggcagaagt
     2341 tgatatttct atattaagta tggttccaag agaatatttt catcctaaac ctaaagtgaa
     2401 tagctcactt atcagattaa gtagaaaaaa atcaagaata tcacacaaag ataaacaaaa
     2461 gtataattat ttcgttatga aatgggttaa caaagaatac aagaaaatat ttacaaaaaa
     2521 tcaatttaac aattccttaa aacatgcagg aattgacgat ttaaacaata ttagctttga
     2581 acaattctta tctcttttca atagctataa attatttaat aagtaagtta agggatgcat
     2641 aaactgcatc ccttaacttg tttttcgtgt gcctattttt tgtgaatcga tctggagctg
     2701 taatataaaa accttcttca actaacgggg caggttagtg acattagaaa accgactgta
     2761 aaaagtacag tcggcattat ctcatattat aaaagccagt cattaggcct atctgacaat
     2821 tcctgaatag agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc
     2881 tgtaaagata gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata
     2941 atgggtagaa ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc
     3001 catggaataa tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc
     3061 cccgaaccat tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca
     3121 ttctttacag gagtccaaat accagagaat gttttagata caccatcaaa a
//
