LOCUS       sequence-445007-        7820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN3-W707AG711E-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..7820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2508
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2528..2593
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2618..2644
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2708..2717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5071..5112
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5122..5139
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5162..5179)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5212..5346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5236..5255)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5290..5309
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5947..6535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6027..6046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6706..7497,7498..7566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7329..7348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7567..7671)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7739..7757
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga aaaagaggga
     1561 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tctgttgcgc
     1621 aaacgcatag actgcactga aaaaatcgat tacctatttt ttatgaatga atatttgcac
     1681 tattactatt caaaactatt aagatagcaa tcacattcaa tagccaaata ctataccacc
     1741 tgagcgatgc aacgaaatga tcaatttgag caaaaatgct gcatatttag gacggcatca
     1801 ttatagaaat gcttcttgct gtgtactttt ctctcgtctg gcagctgttt cgccgttatt
     1861 gttaaaaccg gcttaagtta ggtgtgtttt ctacgactag tgaatgccct actagaagat
     1921 gtgtgttgca caaaatgtcc ctggaataac caatttgaag tgcagatagc agtaaacgta
     1981 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2041 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2101 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2161 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2221 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2281 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2341 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2401 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2461 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2521 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2581 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2641 cgctcctcca agcttgaatt ctatgtgttc cacatacggc ctgctttcat tcgaattctc
     2701 ggacaccgcc gccatggacc cgatcttttt ttccccgacc aacggcattc ccagtgaatc
     2761 gaagctggct acatacatga accgccaaaa cgtggctacg ccctccggat acggtttgaa
     2821 caatggattt agtcttctca acctggactc cccgctcaac aagaaaagcc aagtgacccc
     2881 ccaatccccg gaatttatcc ccacccgtct cggatccacc ccgaattttt acgccccgta
     2941 ccataatgcc ccgatgcagc tatcgaacgg actgaacgga gtgaacggtc taacggctgc
     3001 tgctgctgcg gccgccgccg ctgctgctgc tgctgctgct gccgtcccga gcagcaccag
     3061 ttccgccagc agcgcttccg tcacgatttc caagaccgct aatggcggag ctagcatgct
     3121 ggctttgcag aaaacggcta gcattgcttc agttacgatt gcccagcaac aaccgcaaca
     3181 cccgcaaaag caacagcaac accccccctc cgtcggaggc ggcgccgtca gcgctgctag
     3241 cgctccgatc gctatctcgg gagccccgcc caacccggct gcggccccct ttgtgtcctc
     3301 catgagcgcc caaaccccct tgaaaggacg aggagctatg atgcgtcaag agtcgccgac
     3361 cgctgctatg atctccggag ccggtccggg cgaaaaatcg ccaccgcacg ggatgacgcc
     3421 gcacggagca tcgcccatac catcagcgct gcccaccagc gtgcaccagg agaacgtagg
     3481 cggcaccata tacttctatc cgactgccaa cgcacagaac agccagcccg tcgtcaattc
     3541 catggtggtg gatggaacgc atccggctct ccatggcgtg agtgctgtgg cgccgatgag
     3601 tgcgggcgtt ccggcggcca tgatgtacac ggggcatgtg tatccgggac cgtcctccaa
     3661 cgtggtcacc atgcagccaa agacgctgct ggagtcggcc ttctttatgc ccgacgagat
     3721 gcgcgccgaa gtcctcgccc gcaacgagat ctctaacctg atcatggacg cagcggaggc
     3781 tgcgcagcac gctctaccgc tggaggtgga gaactaccat gccctgtatc cactggaacc
     3841 gccggcgcag ccgttgcacg ccaagctcac gttccccgcc accacttata gggccacgca
     3901 caacacgacg ggctacaagt actgcctgcg aagaatacat ggtttccgcc tgcagtcgac
     3961 caagtgcatg acgctggtgg agatgtggaa gaagctgcag cacacgaatg tggtacaact
     4021 gcgtgaggtg ttcacgacga aagcatttgg tgataactct ttagtattag tgtacgacta
     4081 tcatcccggc tcacaaacat tactggccaa atacttcact ccggcgccag agaccaacgg
     4141 gtacactgat ccattccaag gcgaggcccg ccccttcagt cataagagta acatgcagcg
     4201 aacgagcaat ggaccgctgc taccggaggc caccatctgg tcgatcatca tgcagctgac
     4261 cgccggctta aaagccatcc accatgccgg tcttgcctgc aaggttctgg atcccacaaa
     4321 gattatagtg acgggcaagc gtgtacggtt tagctcatgc tgcatttcgg atataacgca
     4381 attcgatccg aatgcatcca atcccttggc gctggttaat atgcatcagc aggacgatct
     4441 gaccgctttg ggtcgcttag tattagcttt ggcctgccgc tgtcttcaat ccgtgcaacg
     4501 cgacaatgtc cagtccagca tcgatatggt cacgcgaaac tactccaccg atctgcgaaa
     4561 ctttattgtt tacctcttca ccaccaataa tcgtcgctcc gtgaccgacc tgatgccgat
     4621 gattggtgcc cgcttctata cgcaactgga tgcactgcag agcaaaattg atatgcagga
     4681 ggacgagttg gccaaggaga tggagaacgg gcggttatat cgcattttgg tcaaacttaa
     4741 tagcatcaac gagcgacccg actttaatct ggactgcact gcgtctgaga ctgaggatag
     4801 atacatgttg aaattattcc gtgattattt gttccattcc gtcactgagg atggccggcc
     4861 ctggctagat cacgcacaca ttgtacaatg cctcaacaag ctggatgctg gctccataga
     4921 gcgcgtgcaa ctgatgtccc gcgacgagca atcggtactc atcgtctcct atgctgaact
     4981 caagaattgt ctggagaacg ccttctccga actgatgtca agtgcggcca actgagcggc
     5041 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     5101 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     5161 gcctcgactg tgccttctaa ggcctgagct cgctgatcag cctcgatcga ggatccagac
     5221 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     5281 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5341 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     5401 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagtcgacc
     5461 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     5521 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     5581 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     5641 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     5701 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     5761 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     5821 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     5881 ccatcaggga cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5941 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6001 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6061 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6121 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6241 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6301 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6361 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6421 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6481 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6541 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6601 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6661 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6721 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6781 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6841 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6901 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6961 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7021 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7081 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7141 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7201 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7261 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7321 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7381 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7441 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7501 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7561 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7621 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7681 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     7741 taggcgtatc acgaggccct ttcgtcttca agaattaatt ctatattcta aaaacacaaa
     7801 tgatacttct aaaaaaaaat
//
