LOCUS       sequence-44501-e        9420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB550
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..32
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             234..263
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(431..1513)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1514..1591)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1661..1677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1694..1710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1728..1739
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4926..5012
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    5046..5067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5082..5087,5088..5105,5106..5112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5120..5136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5155..5166
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5218..5271
                     /codon_start=1
                     /product="GroES chaperone mobile loop that interacts with 
                     GroEL"
                     /label=S loop
                     /translation="EVETKSAGGIVLTGSAAA"
     terminator      7176..7270
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      7286..7372
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(7407..7424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7541..8086)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7621..7640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8200..8294)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8338..9129)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacgtctgtg tggaattgtg agcggataac aaattcacac agggccctcg gacaccgagg
       61 agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac
      121 ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca
      181 aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa
      241 agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg
      301 aacagctacg gaactcttgt gcgtaaggaa aagtaaggaa aacgattcct tctaacagaa
      361 atgtcctgag caatcaccta tgaactgtcg actcgacaat tcgcgctaac ttacattaat
      421 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
      541 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
      601 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
      661 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
      721 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
      781 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
      841 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
      901 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
      961 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     1021 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     1081 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     1141 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     1201 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     1261 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     1321 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     1381 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     1441 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     1501 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     1561 cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat gcatgccgct tcgccttcgc
     1621 gcgcgaattg tcgagaaaat ttatcaaaaa gagtgttgac ttgtgagcgg ataacaatga
     1681 tacttagatt caattgtgag cggataacaa tttcacacag aattcataaa gaggagaaag
     1741 gatccatggg taaaataatt ggtatcgacc tgggtactac caactcttgt gtagcgatta
     1801 tggatggcac cactcctcgc gtgctggaga acgccgaagg cgatcgcacc acgccttcta
     1861 tcattgccta tacccaggat ggtgaaactc tagttggtca gccggctaaa cgtcaggcag
     1921 tgacgaaccc gcaaaacact ctgtttgcga ttaaacgcct gattggtcgc cgcttccagg
     1981 acgaagaagt acagcgtgat gtttccatca tgccgttcaa aattattgct gctgataacg
     2041 gcgacgcatg ggtcgaagtt aaaggccaga aaatggcacc gccgcagatt tctgctgaag
     2101 tgctgaaaaa aatgaagaaa accgctgaag attacctggg tgaaccggta actgaagctg
     2161 ttatcaccgt accggcatac tttaacgatg ctcagcgtca ggcaaccaaa gacgcaggcc
     2221 gtatcgctgg tctggaagta aaacgtatca tcaacgaacc gaccgcagct gcgctggctt
     2281 acggtctgga caaaggcact ggcaaccgta ctatcgcggt ttatgacctg ggtggtggta
     2341 ctttcgatat ttctattatc gaaatcgacg aagttgacgg cgaaaaaacc ttcgaagttc
     2401 tggcaaccaa cggtgatacc cacctggggg gtgaagactt cgacagccgt ctgatcaact
     2461 atctggttga agaattcaag aaagatcagg gcattgacct gcgcaacgat ccgctggcaa
     2521 tgcagcgcct gaaagaagcg gcagaaaaag cgaaaatcga actgtcttcc gctcagcaga
     2581 ccgacgttaa cctgccatac atcactgcag acgcgaccgg tccgaaacac atgaacatca
     2641 aagtgactcg tgcgaaactg gaaagcctgg ttgaagatct ggtaaaccgt tccattgagc
     2701 cgctgaaagt tgcactgcag gacgctggcc tgtccgtatc tgatatcgac gacgttatcc
     2761 tcgttggtgg tcagactcgt atgccaatgg ttcagaagaa agttgctgag ttctttggta
     2821 aagagccgcg taaagacgtt aacccggacg aagctgtagc aatcggtgct gctgttcagg
     2881 gtggtgttct gactggtgac gtaaaagacg tactgctgct ggacgttacc ccgctgtctc
     2941 tgggtatcga aaccatgggc ggtgtgatga cgacgctgat cgcgaaaaac accactatcc
     3001 cgaccaagca cagccaggtg ttctctaccg ctgaagacaa ccagtctgcg gtaaccatcc
     3061 atgtgctgca gggtgaacgt aaacgtgcgg ctgataacaa atctctgggt cagttcaacc
     3121 tagatggtat caacccggca ccgcgcggca tgccgcagat cgaagttacc ttcgatatcg
     3181 atgctgacgg tatcctgcac gtttccgcga aagataaaaa cagcggtaaa gagcagaaga
     3241 tcaccatcaa ggcttcttct ggtctgaacg aagatgaaat ccagaaaatg gtacgcgacg
     3301 cagaagctaa cgccgaagct gaccgtaagt ttgaagagct ggtacagact cgcaaccagg
     3361 gcgaccatct gctgcacagc acccgtaagc aggttgaaga agcaggcgac aaactgccgg
     3421 ctgacgacaa aactgctatc gagtctgcgc tgactgcact ggaaactgct ctgaaaggtg
     3481 aagacaaagc cgctatcgaa gcgaaaatgc aggaactggc acaggtttcc cagaaactga
     3541 tggaaatcgc ccagcagcaa catgcccagc agcagactgc cggtgctgat gcttctgcaa
     3601 acaacgcgaa agatgacgat gttgtcgacg ctgaatttga agaagtcaaa gacaaaaaat
     3661 aatcgcccta taaacgggta attatactga cacgggcgaa ggggaatttc ctctccgccc
     3721 gtgcattcat ctaggggcaa tttaaaaaag atggctaagc aagattatta cgagatttta
     3781 ggcgtttcca aaacagcgga agagcgtgaa atcagaaagg cctacaaacg cctggccatg
     3841 aaataccacc cggaccgtaa ccagggtgac aaagaggccg aggcgaaatt taaagagatc
     3901 aaggaagctt atgaagttct gaccgactcg caaaaacgtg cggcatacga tcagtatggt
     3961 catgctgcgt ttgagcaagg tggcatgggc ggcggcggtt ttggcggcgg cgcagacttc
     4021 agcgatattt ttggtgacgt tttcggcgat atttttggcg gcggacgtgg tcgtcaacgt
     4081 gcggcgcgcg gtgctgattt acgctataac atggagctca ccctcgaaga agctgtacgt
     4141 ggcgtgacca aagagatccg cattccgact ctggaagagt gtgacgtttg ccacggtagc
     4201 ggtgcaaaac caggtacaca gccgcagact tgtccgacct gtcatggttc tggtcaggtg
     4261 cagatgcgcc agggattctt cgctgtacag cagacctgtc cacactgtca gggccgcggt
     4321 acgctgatca aagatccgtg caacaaatgt catggtcatg gtcgtgttga gcgcagcaaa
     4381 acgctgtccg ttaaaatccc ggcaggggtg gacactggag accgcatccg tcttgcgggc
     4441 gaaggtgaag cgggcgagca tggcgcaccg gcaggcgatc tgtacgttca ggttcaggtt
     4501 aaacagcacc cgattttcga gcgtgaaggc aacaacctgt attgcgaagt cccgatcaac
     4561 ttcgctatgg cggcgctggg tggcgaaatc gaagtaccga cccttgatgg tcgcgtcaaa
     4621 ctgaaagtgc ctggcgaaac ccagaccggt aagctattcc gtatgcgcgg taaaggcgtc
     4681 aagtctgtcc gcggtggcgc acagggtgat ttgctgtgcc gcgttgtcgt cgaaacaccg
     4741 gtaggcctga acgaaaggca gaaacagctg ctgcaagagc tgcaagaaag cttcggtggc
     4801 ccaaccggcg agcacaacag cccgcgctca aagagcttct ttgatggtgt gaagaagttt
     4861 tttgacgacc tgacccgcta agatgtcgac ctgcagccaa gcttaattag ctgatctaga
     4921 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     4981 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag tcgaggcaac
     5041 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     5101 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacagaatt cattaaagag
     5161 gagaaattaa ccatgaatat tcgtccattg catgatcgcg tgatcgtcaa gcgtaaagaa
     5221 gttgaaacta aatctgctgg cggcatcgtt ctgaccggct ctgcagcggc taaatccacc
     5281 cgcggcgaag tgctggctgt cggcaatggc cgtatccttg aaaatggcga agtgaagccg
     5341 ctggatgtga aagttggcga catcgttatt ttcaacgatg gctacggtgt gaaatctgag
     5401 aagatcgaca atgaagaagt gttgatcatg tccgaaagcg acattctggc aattgttgaa
     5461 gcgtaatccg cgcacgacac tgaacatacg aatttaagga ataaagataa tggcagctaa
     5521 agacgtaaaa ttcggtaacg acgctcgtgt gaaaatgctg cgcggcgtaa acgtactggc
     5581 agatgcagtg aaagttaccc tcggtccaaa aggccgtaac gtagttctgg ataaatcttt
     5641 cggtgcaccg accatcacca aagatggtgt ttccgttgct cgtgaaatcg aactggaaga
     5701 caagttcgaa aatatgggtg cgcagatggt gaaagaagtt gcctctaaag caaacgacgc
     5761 tgcaggcgac ggtaccacca ctgcaaccgt actggctcag gctatcatca ctgaaggtct
     5821 gaaagctgtt gctgcgggca tgaacccgat ggacctgaaa cgtggtatcg acaaagcggt
     5881 taccgctgca gttgaagaac tgaaagcgct gtccgtacca tgctctgact ctaaagcgat
     5941 tgctcaggtt ggtaccatct ccgctaactc cgacgaaacc gtaggtaaac tgatcgctga
     6001 agcgatggac aaagtcggta aagaaggcgt tatcaccgtt gaagacggta ccggtctgca
     6061 ggacgaactg gacgtggttg aaggtatgca gttcgaccgt ggctacctgt ctccttactt
     6121 catcaacaag ccggaaactg gcgcagtaga actggaaagc ccgttcatcc tgctggctga
     6181 caagaaaatc tccaacatcc gcgaaatgct gccggttctg gaagctgttg ccaaagcagg
     6241 caaaccgctg ctgatcatcg ctgaagatgt agaaggcgaa gcgctggcaa ctctggttgt
     6301 taacaccatg cgtggcatcg tgaaagtcgc tgcggttaaa gcaccgggct tcggcgatcg
     6361 tcgtaaagct atgctgcagg atatcgcaac cctgactggc ggtaccgtga tctctgaaga
     6421 gatcggtatg gagctggaaa aagcaaccct ggaagacctg ggtcaggcta aacgtgttgt
     6481 gatcaacaaa gacaccacca ctatcatcga tggcgtgggt gaagaagctg caatccaggg
     6541 ccgtgttgct cagatccgtc agcagattga agaagcaact tctgactacg accgtgaaaa
     6601 actgcaggaa cgcgtagcga aactggcagg cggcgttgca gttatcaaag tgggtgctgc
     6661 taccgaagtt gaaatgaaag agaaaaaagc acgcgttgaa gatgccctgc acgcgacccg
     6721 tgctgcggta gaagaaggcg tggttgctgg tggtggtgtt gcgctgatcc gcgtagcgtc
     6781 taaactggct gacctgcgtg gtcagaacga agaccagaac gtgggtatca aagttgcact
     6841 gcgtgcaatg gaagctccgc tgcgtcagat cgtattgaac tgcggcgaag aaccgtctgt
     6901 tgttgctaac accgttaaag gcggcgacgg caactacggt tacaacgcag caaccgaaga
     6961 atacggcaac atgatcgaca tgggtatcct ggatccaacc aaagtaactc gttctgctct
     7021 gcagtacgca gcttctgtgg ctggcctgat gatcaccacc gaatgcatgg ttaccgacct
     7081 gccgaaaaac gatgcagctg acttaggcgc tgctggcggt atgggcggca tgggtggcat
     7141 gggcggcatg atgtaagctt aattagctga gcttggactc ctgttgatag atccagtaat
     7201 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
     7261 tggtgagaat ccaagctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     7321 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     7381 ctagacctag ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     7441 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     7501 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     7561 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     7621 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     7681 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     7741 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     7801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     7861 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     7921 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     7981 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     8041 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     8101 taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga
     8161 agtcagcccc atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc
     8221 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     8281 tatcaacagg agtccaagcg agctcgatat ccgtcggctt gaacgaattg ttagacatta
     8341 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
     8401 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
     8461 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
     8521 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
     8581 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
     8641 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     8701 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     8761 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     8821 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
     8881 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
     8941 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
     9001 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
     9061 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
     9121 ttccctcatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
     9181 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
     9241 catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     9301 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     9361 ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccggatatc
//
