LOCUS       sequence-445011-        9459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not 
            contain an HA-tag as the name implies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Zekri L, Huntzinger E, Heimstadt S, Izaurralde E
  TITLE     The silencing domain of GW182 interacts with PABPC1 to promote 
            translational repression and degradation of microRNA targets and is 
            required for target release.
  JOURNAL   Mol Cell Biol. 2009 Dec;29(23):6220-31. doi: 10.1128/MCB.01081-09. 
            Epub 2009 Sep 21.
  PUBMED    19797087
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01081-09"; journalName: "Mol Cell Biol"; date: 
            "2009-12"; volume: "29"; issue: "23"; pages: "6220-31"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2531..2596
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             6710..6751
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6761..6778
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6801..6818)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6851..6985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6875..6894)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6929..6948
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7586..8174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7666..7685)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8345..9136,9137..9205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8968..8987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9206..9310)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9378..9396
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtacccaac atggacgcac aaacacgacg acgtgagcgt cgcgctgaga aacaagctca
     2581 atggaaagct gcaaacggtg gatctcctcc aagcttgaat tctgcagata tccagcacag
     2641 tggcggccgc atgactgatg ctccttcggt tgaaatatta ccaacgcccc aaagagggag
     2701 aagcaaaaaa attcctatag tgtctcctaa aaatgtatct gagtccatag ttgctcctat
     2761 cactgatgaa acggatgatg cagggtcaac accgatatcc agagccaccg aagaatctat
     2821 gccacctaat cagacccatc caaatttact catatccgat tcatcaaaac ataagcaagc
     2881 agtatcaaat agtgagattt caaaagacgc tcctactggt ttaaaggaaa tgcatgttgc
     2941 tgagctggag tccagtgtag cctccgaaaa tcatccagat ggaatactag atgtcaatgt
     3001 caacaagagt tcacaatcat cggatttttc tgcagatccc gaaattgata gtattggcgc
     3061 aatatcacct acgagtccaa acgcagtttc tcctatttta cacgaagttt tagataatac
     3121 tgagttgtca aaaaaattag agaattctac gacagaaaga tttaaagata gccaaagcgt
     3181 agagaagcca actcatcaag aactaagttt aagaaatgcc acagacgaga ctgaaatatc
     3241 cgcaatggca ttacaagatg tgaatagttt ggataataac caaattgaaa aaacataccc
     3301 ttcaaagccg aaactcaatg tagatgtttc agaagacatt tcctccagag agtcagcaat
     3361 aaagtcaact tctacaaaaa atacaggagt agatgtaggt ctccaatcag attctaagcc
     3421 agagactacc cttaacgata aacaggactc tactgaccta aaagtgaaag tgtctgcaaa
     3481 aatttcatct atcataaact ataatgaggg tcaatggtca ccaaacaatc caagcggaaa
     3541 aaaacaatac gaccgagaac agttattaca gttacgcgag gttaaggcct cacgtataca
     3601 accagaggta aaaaatgtgt ctattcttcc tcaaccaaac ttaatgccat catttattcg
     3661 aaataacaat aataacaagc gagtacaatc gatggttggt attattggca atcgtagcaa
     3721 tgaatcggcg ggaaattata ttggtaaaca gatgtcgatg tctggcgtac aaagtggcgg
     3781 cggtagaagc agtatgaagg gcatgataca cgtaaattta tcattaaatc aagatgtaaa
     3841 gttaagtgaa aatgaaaatg catggcgccc tcgggtctta aataaatctg atggtgatag
     3901 cgacgcgaaa tctgcactcg aaaaagatga attggtacgt cgtgttcggg gtattttaaa
     3961 taagcttacc ccagaaaggt ttgacacact tgttgaggag attataaaat tgaaaattga
     4021 tacacccgac aaagtggacg aagtaattgt gttggtattt gaaaaggcca ttgatgagcc
     4081 aaacttttct gtgtcttacg ccagactatg tcaacgactt gctgcggaag tgaaggtaat
     4141 agacgagcgc atggaaagcg aaaccaaatc taactcggca cactttcgaa atgcattgtt
     4201 agataaaact gaacaggaat tcacccaaaa cgtttcacaa agcaccgcaa aggaaaaaaa
     4261 attacagcca attgtggata aaattaagaa atgtactgat gccaatgaaa aagcggaact
     4321 tgaggcattt ttggaagaag aagaaagaaa aataagaaga cgatccggag ggacagtacg
     4381 atttattggc gaacttttta agatatctat gttgactggc aaaataatat actcttgcat
     4441 cgatacactt cttaaccctc attctgaaga catgcttgag tgtttgtgca agctgcttac
     4501 aactgtgggt gctaaatttg aaaagacccc agtaaattcc aaagacccta gtcggtgtta
     4561 ttcgttggaa aaatcgatca ccaggatgca ggccatcgca tcgaaaactg acaaagatgg
     4621 agccagagta agctcaagag tacgatttat gcttcaagac gtcatcgact tgcgtaagaa
     4681 taaatggcaa acttcgcgga atgaagcacc caaaactatg ggacaaattg aaaaagaggc
     4741 aaagaatgag cagctttctg cacaatattt tggtacactt tccagtacta cacccggagg
     4801 atcgcagggc ggctctggta aacgagatga ccgtggaaac tctcgatacg gtgaatctcg
     4861 ttcaagtagc gcgtatggtg gcagccacag tcaacgtggt gataatggca accttcgaca
     4921 ccagcagcag aacaacgtcg ggggaaacgt ctctggaggt gctggacact ccaatggaaa
     4981 caatgatgaa aatacttggc atgtgcaaac cagcaaaggt agccgctctc tagcggttga
     5041 cagcaataaa ctggaaggcc tgtctaaact ttctgaccaa aatctggaaa caaagaagat
     5101 gggtggattg acgcaattca tttggattag tagtgacacg acaagactat cgtcggcgcc
     5161 gacaccaacc ccatccaatc ctttcgctgt gctctcatcc ctaattgata aaaatagtaa
     5221 tgaaagggat cgcgatcgca gtggacccag aaacaaagga tcatacaaca aaggttctat
     5281 ggagcgggat cgttatgata gaggtatgca ttcgagaact ggatcatctc agggatcacg
     5341 agaaaactca tcctcccgcg gtggtcagca gggtcgaact ttattgagtt catcagttca
     5401 aaaatctact agtcattcaa aatacaccca acaggcacca cctacacggc atactgttaa
     5461 agcccagtca tcagtgggca gctctaacgt taacacaggt ccactctacc gtggaagtga
     5521 gcagcaaaca tcagcaacct tctcgcagac cacccgttct gttgctcctg tagcagtgtt
     5581 catcgaagct agcgaaacag atttaaaact catcaaatct gttgtttcag aaattgtaga
     5641 cctatcagct gcatctaaag aagtcacgcc cggtgcagtt tcgtgcataa aacgagtacc
     5701 agagaaacta cggtgcagtt tcatttatta tattttaaca gattatttgc atttagcaaa
     5761 tgtaggtaaa cagtacaggc ggtatttatc tattgccgtc tcccaactta ttcagcagaa
     5821 ttacatttct gccgatcatt tgagactggc ttacaacgaa tttaccgtat atgcaaacga
     5881 tttgattgtt gatattccgg agctttggct atacattctt caatttgccg ggccactaat
     5941 tgtaaagaaa atattgacta tatcagattt gtggaacaac aatttaaaag aaaatagtcc
     6001 atcaaacgtc gctaaaaagt tccttaaaac atacttgata tattgcacac aagaagttgg
     6061 accaaatttt gctcggaata tgtggatcaa gtttaaccta aaatggtcag attttatgcc
     6121 tgaaagtgaa gttgccgact ttattaaatt taacagactg gaatatgtgg aaaacgaatc
     6181 aaagtcgcca gtgattgatc atagagagac tccagaaaag cacgttaaaa atgtgataga
     6241 ccatattgag cacttactaa aggaaggaac aacggcggac tgcattattg attatagtaa
     6301 cggaaatata atggtggttg ataagttgtt tatcagagga ttgacggaaa cattgagtaa
     6361 tttctcgatt cactacaagg acaacagtta caagcttgag tccgaaacct ttcaaaagtt
     6421 ttgcataccg gttttgcaga gatacattga ttctaatgaa gaccaccaac ttgaatgttt
     6481 atatactttg cagcttttag ttcatggatt ggagcatcca agagggttgc tgagtgaact
     6541 aattggcgag ctatatgatg cctttgtaat acagaaggaa tcgctttgta agtggcgaga
     6601 ttcaaaggat cagtctgctg gcaagggcgt tgctgtaaaa agtcttaatc ctttttttaa
     6661 ctcgttgtta aacgatgatg ccaacagtct agagggcccg cggttcgaag gtaagcctat
     6721 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     6781 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     6841 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6901 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6961 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     7021 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     7081 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     7141 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     7201 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     7261 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     7321 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     7381 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     7441 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     7501 ccaggcaggt agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc
     7561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8641 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8941 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     9001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9361 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattaattc
     9421 tatattctaa aaacacaaat gatacttcta aaaaaaaat
//
